Identification of 4CL gene family in peach and its expression analysis in fruit coloration during development stage and chilling injury during post-harvest low temperature storage

doi:10.3969/j.issn.1004-1524.20221604

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (11): 2600-2610.DOI: 10.3969/j.issn.1004-1524.20221604

• Horticultural Science • Previous Articles Next Articles

Identification of 4CL gene family in peach and its expression analysis in fruit coloration during development stage and chilling injury during post-harvest low temperature storage

KONG Fanwang¹^,²(), ZHANG Zhigang², LI Wei², CHEN Yufeng², WANG Changjiang², ZHENG Yaqin¹, XU Meng¹^,^*()

1. College of Agriculture and Forestry Sciences, Linyi University, Linyi 276000, Shandong, China
2. Zoucheng Natural Resources and Planning Bureau, Jining 273500, Shandong, China

Received:2022-11-11 Online:2023-11-25 Published:2023-12-04

Abstract

Abstract:

4CL (4-coumarate: CoA ligase) plays an important role in the metabolism of phenylalanine compounds such as flavonoids and lignin. In this study, the peach 4CL gene family members were systematically identified based on the peach genome database, and the structure and possible functions of the peach 4CLs were analyzed using the genetic family evolutionary analysis, combing transcriptome data and qRT-PCR techniques to analyze their functions in flavonoids synthesis at the fruit development stage and chilling-induced lignification regulation at postharvest. The results showed that 21 members of the 4CL gene family, namely Pp4CL1-Pp4CL21, were identified on 8 chromosomes based on the peach genome database. Phylogenetic analysis showed that Pp4CL1 and Pp4CL2 belong to the first subfamily, mainly involved in the synthesis of lignin, and Pp4CL3-Pp4CL21 belongs to the second subfamily, mainly involved in the synthesis of flavonoids. The cis-element analysis of the 4CL promoters found that it contains a large number of abiotic stress and MeJA-responsive and other hormone response elements. The gene expression of different peach varieties at the development stage showed that Pp4CL8 and Pp4CL13 increased generally, and higher expression was observed in blood-flesh varieties than in yellow-and milk-white-flesh varieties, suggesting that these genes may participate in the fruit flavonoids synthesis. The gene expression of peach fruit during post-harvest cold storage showed that Pp4CL2 may play a role in the chilling-induced lignification of fruit flesh after harvest. After low temperature conditioning (LTC) treatment, Pp4CL8, Pp4CL10 and Pp4CL14 may reduce the chilling injury of peach fruit during post-harvest cold storage by improving flavonoids content. This study provides a theoretical basis for the coloration of peach fruit and its postharvest preservation.

Key words: peach, lignin, flavonoids, gene expression

CLC Number:

S662.1

KONG Fanwang, ZHANG Zhigang, LI Wei, CHEN Yufeng, WANG Changjiang, ZHENG Yaqin, XU Meng. Identification of 4CL gene family in peach and its expression analysis in fruit coloration during development stage and chilling injury during post-harvest low temperature storage[J]. Acta Agriculturae Zhejiangensis, 2023, 35(11): 2600-2610.

Figures/Tables 11

References 38

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基因名称 Gene name	登录号 GenBank	氨基酸数目 Amino acid	等电点 Isoelectric point	分子量 Molecular weight/u	不稳定系数 Instability index
Pp4CL1	XM_007222180.2	544	5.83	59 937.30	30.32
Pp4CL2	XM_007215457.2	551	5.84	59 981.39	37.07
Pp4CL3	XM_020570163.1	567	8.9	62 153.90	41.82
Pp4CL4	XM_020567696.1	572	6.32	62 409.91	38.71
Pp4CL5	XM_007219446.2	457	5.56	49 467.21	31.17
Pp4CL6	XM_007225695.2	548	6.06	59 512.92	36.62
Pp4CL7	XM_007203554.2	543	8.44	59 630.94	41.21
Pp4CL8	XM_007208395.2	542	8.63	59 168.68	37.81
Pp4CL9	XM_007222403.2	569	8.95	61 299.59	33.63
Pp4CL10	XM_007209025.2	557	7.26	61 211.61	53.40
Pp4CL11	XM_020569127.1	591	8.47	63 537.10	43.54
Pp4CL12	XM_007219420.2	569	5.73	61 733.01	42.35
Pp4CL13	XM_007209024.2	558	6.26	61 052.43	49.46
Pp4CL14	XM_020566011.1	635	6.56	69 746.76	36.41
Pp4CL15	XM_007221216.2	748	8.29	83 920.25	44.22
Pp4CL16	XM_020554905.1	748	8.29	83 920.25	44.22
Pp4CL17	XM_020554134.1	566	8.11	63 179.53	37.96
Pp4CL18	XM_020561814.1	555	7.23	60 667.33	38.26
Pp4CL19	XM_020564772.1	165	6.51	18 632.33	44.38
Pp4CL20	XM_007200387.2	218	6.6	25 006.86	38.89
Pp4CL21	XM_020570041.1	218	6.6	25 006.86	38.89

基因名称 Gene name	登录号 GenBank	氨基酸数目 Amino acid	等电点 Isoelectric point	分子量 Molecular weight/u	不稳定系数 Instability index
Pp4CL1	XM_007222180.2	544	5.83	59 937.30	30.32
Pp4CL2	XM_007215457.2	551	5.84	59 981.39	37.07
Pp4CL3	XM_020570163.1	567	8.9	62 153.90	41.82
Pp4CL4	XM_020567696.1	572	6.32	62 409.91	38.71
Pp4CL5	XM_007219446.2	457	5.56	49 467.21	31.17
Pp4CL6	XM_007225695.2	548	6.06	59 512.92	36.62
Pp4CL7	XM_007203554.2	543	8.44	59 630.94	41.21
Pp4CL8	XM_007208395.2	542	8.63	59 168.68	37.81
Pp4CL9	XM_007222403.2	569	8.95	61 299.59	33.63
Pp4CL10	XM_007209025.2	557	7.26	61 211.61	53.40
Pp4CL11	XM_020569127.1	591	8.47	63 537.10	43.54
Pp4CL12	XM_007219420.2	569	5.73	61 733.01	42.35
Pp4CL13	XM_007209024.2	558	6.26	61 052.43	49.46
Pp4CL14	XM_020566011.1	635	6.56	69 746.76	36.41
Pp4CL15	XM_007221216.2	748	8.29	83 920.25	44.22
Pp4CL16	XM_020554905.1	748	8.29	83 920.25	44.22
Pp4CL17	XM_020554134.1	566	8.11	63 179.53	37.96
Pp4CL18	XM_020561814.1	555	7.23	60 667.33	38.26
Pp4CL19	XM_020564772.1	165	6.51	18 632.33	44.38
Pp4CL20	XM_007200387.2	218	6.6	25 006.86	38.89
Pp4CL21	XM_020570041.1	218	6.6	25 006.86	38.89

Identification of 4CL gene family in peach and its expression analysis in fruit coloration during development stage and chilling injury during post-harvest low temperature storage

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