The directional transfer of the plain silkworm variety Jingsong into a sex-limited multilunar silkworm variety

doi:10.3969/j.issn.1004-1524.20230794

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (5): 1032-1041.DOI: 10.3969/j.issn.1004-1524.20230794

• Animal Science • Previous Articles Next Articles

The directional transfer of the plain silkworm variety Jingsong into a sex-limited multilunar silkworm variety

LIAO Pengfei(), LI Qiongyan, LUO Shungao, LIU Min, ZHU Hongtao, LI Jiya, BAI Hongying, CHEN Haiquan, FAN Yonghui, DONG Zhanpeng^*()

Sericultural & Apicultural Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi 661101, Yunnan, China

Received:2023-06-26 Online:2024-05-25 Published:2024-05-29

Abstract

Abstract:

To identify the gender of the larvae in the early developmental stages of the current plain silkworm variety Jingsong, for the purpose of achieving sex-separation in rearing and differential utilization, the female of the sex-limited normal marking silkworm race Yuncan 7A was crossed with the male of multilunar silkworm race Cb. Then, these hybrids were respectively crossed with the commercial plain silkworm race Jingsong A and Jingsong B. The progeny were backcrossed to the commercial plain silkworm race for several times and then selfed, combined with categorized selection for individual and batches. This process introduced sex-limited normal marking gene of Yuncan 7A and multilunar gene of Cb into the commercial plain silkworm race Jingsong, directed breeding resulted in Jing A Chaxian and Jing B Chaxian. The results showed that during the breeding process, the larval spot differentiation turned into easy-to-distinguish multilunar and non-multilunar types by color and shape. The cultivated Jing A Chaxian and Jing B Chaxian larvae displayed clearly different color differentiation of multilunar at the beginning of the 3rd instar, allowing for accurate gender identification. The economic traits of Jing A Chaxian and Jing B Chaxian, such as the larva-pupa rate of 96.91% and 96.40%, the whole cocoon weight of 1.62 g and 1.56 g, the cocoon shell ratio of 23.78% and 23.26%, were slightly different from their recurrent parents, exhibiting characteristics of good health, easy rearing, and high-quality cocoons. This study laid a foundation for creating breeding materials with special traits, the differential utilization of male and female silkworms, and reducing the breeding costs of male silkworms.

Key words: silkworm race, sex-limited normal marking, plain, sex-limited multilunar, directional transfer

CLC Number:

S882

LIAO Pengfei, LI Qiongyan, LUO Shungao, LIU Min, ZHU Hongtao, LI Jiya, BAI Hongying, CHEN Haiquan, FAN Yonghui, DONG Zhanpeng. The directional transfer of the plain silkworm variety Jingsong into a sex-limited multilunar silkworm variety[J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 1032-1041.

Figures/Tables 6

References 21

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年份 Year	蚕期 Silkworm rearing season	世代 Generation	饲育形式 Rearing form	饲育数量/区 Rearing amount/ batch	5龄期经过 Duration of 5^th instar/h	全龄龄期经过 Larval duration/h	死笼率 Dead cocoons ratio/%	虫蛹率 Larva- pupa rate/%	全茧量 Cocoon weight/g	茧层量 Cocoon shell weight/g	茧层率 Cocoon shell ratio/%
2015	春Spring	P₁(云蚕7A Yuncan 7A)	○	1	204	648	4.16	94.47	1.93	0.44	22.99
		P₂(Cb)	○	1	132	540	4.00	85.38	1.16	0.15	12.72
	秋Autumn	P₃(菁松A Jingsong A)	○	1	174	611	2.50	95.91	1.66	0.39	23.63
		F₁(云蚕7A×Cb	○	1	150	560	3.82	91.76	1.69	0.31	18.07
		Yuncan 7A×Cb)
	晚秋Late autumn	F2(云蚕7A·Cb×菁松A	○	1	174	612	6.11	90.49	1.65	0.28	16.94
		Yuncan 7A·Cb×Jingsong A)
2016	春Spring	BC₁	□	12	175	606	1.07	97.05	1.75	0.39	22.37
	秋Autumn	BC₂	○	1	169	606	7.50	91.80	1.81	0.37	20.62
	晚秋Late autumn	BC₃	○	1	188	678	4.76	73.04	1.04	0.19	18.70
2017	春Spring	BC₄	○	1	174	654	1.66	92.45	1.45	0.28	19.54
	秋Autumn	BC₅	○	1	161	580	1.23	96.05	1.79	0.35	19.55
	晚秋Late autumn	BC₆	○	1	198	660	10.25	78.69	1.32	0.25	19.01
2018	春Spring	S₁	○	1	212	678	5.41	73.55	1.56	0.33	21.25
	秋Autumn	S₂	□	16	150	582	2.35	94.66	1.54	0.33	21.43
	晚秋Late autumn	S₃	○	13	180	666	3.02	90.51	1.38	0.28	20.29
2019	春Spring	S₄	○	4	201	662	9.00	81.91	1.69	0.39	22.95
	秋Autumn	S₅	□	16	164	582	2.25	93.56	1.55	0.36	23.12
2020	春Spring	S₆	□	16	216	705	15.41	72.33	1.69	0.38	22.19
	秋Autumn	S₇	□	16	183	610	0.79	98.06	1.69	0.41	24.10
2021	春Spring	S₈	□	16	175	618	1.42	95.76	1.55	0.36	23.46
	秋Autumn	S₉	□	12	191	644	2.63	93.86	1.48	0.33	22.31

年份 Year	蚕期 Silkworm rearing season	世代 Generation	饲育形式 Rearing form	饲育数量/区 Rearing amount/ batch	5龄期经过 Duration of 5^th instar/h	全龄龄期经过 Larval duration/h	死笼率 Dead cocoons ratio/%	虫蛹率 Larva- pupa rate/%	全茧量 Cocoon weight/g	茧层量 Cocoon shell weight/g	茧层率 Cocoon shell ratio/%
2015	春Spring	P₁(云蚕7A Yuncan 7A)	○	1	204	648	4.16	94.47	1.93	0.44	22.99
		P₂(Cb)	○	1	132	540	4.00	85.38	1.16	0.15	12.72
	秋Autumn	P₃(菁松A Jingsong A)	○	1	174	611	2.50	95.91	1.66	0.39	23.63
		F₁(云蚕7A×Cb	○	1	150	560	3.82	91.76	1.69	0.31	18.07
		Yuncan 7A×Cb)
	晚秋Late autumn	F2(云蚕7A·Cb×菁松A	○	1	174	612	6.11	90.49	1.65	0.28	16.94
		Yuncan 7A·Cb×Jingsong A)
2016	春Spring	BC₁	□	12	175	606	1.07	97.05	1.75	0.39	22.37
	秋Autumn	BC₂	○	1	169	606	7.50	91.80	1.81	0.37	20.62
	晚秋Late autumn	BC₃	○	1	188	678	4.76	73.04	1.04	0.19	18.70
2017	春Spring	BC₄	○	1	174	654	1.66	92.45	1.45	0.28	19.54
	秋Autumn	BC₅	○	1	161	580	1.23	96.05	1.79	0.35	19.55
	晚秋Late autumn	BC₆	○	1	198	660	10.25	78.69	1.32	0.25	19.01
2018	春Spring	S₁	○	1	212	678	5.41	73.55	1.56	0.33	21.25
	秋Autumn	S₂	□	16	150	582	2.35	94.66	1.54	0.33	21.43
	晚秋Late autumn	S₃	○	13	180	666	3.02	90.51	1.38	0.28	20.29
2019	春Spring	S₄	○	4	201	662	9.00	81.91	1.69	0.39	22.95
	秋Autumn	S₅	□	16	164	582	2.25	93.56	1.55	0.36	23.12
2020	春Spring	S₆	□	16	216	705	15.41	72.33	1.69	0.38	22.19
	秋Autumn	S₇	□	16	183	610	0.79	98.06	1.69	0.41	24.10
2021	春Spring	S₈	□	16	175	618	1.42	95.76	1.55	0.36	23.46
	秋Autumn	S₉	□	12	191	644	2.63	93.86	1.48	0.33	22.31

年份 Year	蚕期 Silkworm rearing season	世代 Generation	饲育形式 Rearing form	饲育数量/区 Rearing amount/ batch	5龄期经过 Duration of 5^th instar/h	全龄龄期经过 Larval duration/h	死笼率 Dead cocoons ratio/%	虫蛹率 Larva- pupa rate/%	全茧量 Cocoon weight/g	茧层量 Cocoon shell weight/g	茧层率 Cocoon shell ratio/%
2015	春Spring	P₁(云蚕7A Yuncan 7A)	○	1	204	648	4.16	94.47	1.93	0.44	22.99
		P₂(Cb)	○	1	132	540	4.00	85.38	1.16	0.15	12.72
	秋Autumn	P₃(菁松B Jingsong B)	○	1	192	635	1.73	96.28	1.64	0.37	22.71
		F₁(云蚕7A×Cb	○	1	150	560	3.82	91.76	1.69	0.31	18.07
		Yuncan 7A×Cb)
	晚秋Late autumn	F₂(云蚕7A·Cb×菁松B	○	1	180	618	5.03	91.25	1.58	0.27	17.09
		Yuncan 7A·Cb×Jingsong B)
2016	春Spring	BC₁	□	12	175	606	1.98	95.33	1.79	0.41	22.72
	秋Autumn	BC₂	○	1	175	612	0.68	97.64	1.48	0.31	20.88
	晚秋Late autumn	BC₃	○	1	188	678	5.00	75.74	1.12	0.22	19.29
2017	春Spring	BC₄	○	1	173	653	1.02	97.68	1.48	0.32	21.62
	秋Autumn	BC₅	○	1	161	580	2.5	94.65	1.78	0.34	19.24
	晚秋Late autumn	BC₆	○	1	198	660	11.27	74.56	1.29	0.25	19.38
2018	春Spring	S₁	○	1	212	678	7.68	83.33	1.56	0.35	22.53
	秋Autumn	S₂	□	16	154	586	3.12	95.02	1.50	0.31	20.31
	晚秋Late autumn	S₃	○	11	184	668	2.79	90.87	1.41	0.29	20.12
2019	春Spring	S₄	○	5	198	659	7.72	82.28	1.61	0.36	22.16
	秋Autumn	S₅	□	16	164	582	1.98	94.65	1.50	0.34	22.69
2020	春Spring	S₆	□	16	228	707	10.53	73.15	1.68	0.35	21.02
	秋Autumn	S₇	□	16	188	617	1.08	96.47	1.60	0.37	23.35
2021	春Spring	S₈	□	16	182	601	1.39	96.32	1.52	0.35	23.16
	秋Autumn	S₉	□	12	186	610	2.56	95.19	1.53	0.34	22.47

年份 Year	蚕期 Silkworm rearing season	世代 Generation	饲育形式 Rearing form	饲育数量/区 Rearing amount/ batch	5龄期经过 Duration of 5^th instar/h	全龄龄期经过 Larval duration/h	死笼率 Dead cocoons ratio/%	虫蛹率 Larva- pupa rate/%	全茧量 Cocoon weight/g	茧层量 Cocoon shell weight/g	茧层率 Cocoon shell ratio/%
2015	春Spring	P₁(云蚕7A Yuncan 7A)	○	1	204	648	4.16	94.47	1.93	0.44	22.99
		P₂(Cb)	○	1	132	540	4.00	85.38	1.16	0.15	12.72
	秋Autumn	P₃(菁松B Jingsong B)	○	1	192	635	1.73	96.28	1.64	0.37	22.71
		F₁(云蚕7A×Cb	○	1	150	560	3.82	91.76	1.69	0.31	18.07
		Yuncan 7A×Cb)
	晚秋Late autumn	F₂(云蚕7A·Cb×菁松B	○	1	180	618	5.03	91.25	1.58	0.27	17.09
		Yuncan 7A·Cb×Jingsong B)
2016	春Spring	BC₁	□	12	175	606	1.98	95.33	1.79	0.41	22.72
	秋Autumn	BC₂	○	1	175	612	0.68	97.64	1.48	0.31	20.88
	晚秋Late autumn	BC₃	○	1	188	678	5.00	75.74	1.12	0.22	19.29
2017	春Spring	BC₄	○	1	173	653	1.02	97.68	1.48	0.32	21.62
	秋Autumn	BC₅	○	1	161	580	2.5	94.65	1.78	0.34	19.24
	晚秋Late autumn	BC₆	○	1	198	660	11.27	74.56	1.29	0.25	19.38
2018	春Spring	S₁	○	1	212	678	7.68	83.33	1.56	0.35	22.53
	秋Autumn	S₂	□	16	154	586	3.12	95.02	1.50	0.31	20.31
	晚秋Late autumn	S₃	○	11	184	668	2.79	90.87	1.41	0.29	20.12
2019	春Spring	S₄	○	5	198	659	7.72	82.28	1.61	0.36	22.16
	秋Autumn	S₅	□	16	164	582	1.98	94.65	1.50	0.34	22.69
2020	春Spring	S₆	□	16	228	707	10.53	73.15	1.68	0.35	21.02
	秋Autumn	S₇	□	16	188	617	1.08	96.47	1.60	0.37	23.35
2021	春Spring	S₈	□	16	182	601	1.39	96.32	1.52	0.35	23.16
	秋Autumn	S₉	□	12	186	610	2.56	95.19	1.53	0.34	22.47

品种名 Variety	斑纹 Dapple	类型 Type	5龄期经过 Duration of 5^th instar/h	全龄龄期经过 Larval duration/h	死笼率 Dead cocoons ratio/%	虫蛹率 Larva- pupa rate/%	全茧量 Cocoon weight/g	茧层量 Cocoon shell weight/g	茧层率 Cocoon shell ratio/%
Cb	茶斑	供体亲本	154	579	4.01	87.55	1.22	0.17	14.31
	Multilunar marking	Donor parent
云蚕7A	普斑限性	供体亲本	207	644	2.18	94.35	1.79	0.40	22.33
Yuncan 7A	Sex-limited normal marking	Donor parent
菁松A Jingsong A	素斑 Plain	受体亲本(轮回亲本) Receptor parent (Reincarnation parent)	174	606	0.91	97.02	1.68	0.40	23.99
菁A茶限 Jing A Chaxian	茶斑限性 Sex-limited multilunar marking	新创建品系 Newly created strain	184	614	1.11	96.91	1.62	0.39	23.78
菁松B Jingsong B	素斑 Plain	受体亲本(轮回亲本) Receptor parent (Reincarnation parent)	188	612	1.98	95.76	1.61	0.37	23.01
菁B茶限 Jing B Chaxian	茶斑限性 Sex-limited multilunar marking	新创建品系 Newly created strain	185	609	1.24	96.40	1.56	0.36	23.26

The directional transfer of the plain silkworm variety Jingsong into a sex-limited multilunar silkworm variety

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