Investigation on virus disease in ‘Niuwei’ yam and removal of virus with stem culture

doi:10.3969/j.issn.1004-1524.20230860

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (6): 1329-1338.DOI: 10.3969/j.issn.1004-1524.20230860

• Plant Protection • Previous Articles Next Articles

Investigation on virus disease in ‘Niuwei’ yam and removal of virus with stem culture

CHENG Yuan^a(), XU Shengsheng^a, DUAN Yanbi^b, XU Xinlei^a, GUO Fenggen^c, WANG Shiyu^a, LONG Wenhong^a^,^*()

a. College of Landscape and Horticulturey, Yunnan Agricultural University, Kunming 650201, China
b. Agricultural Science Teaching and Experiment Centery, Yunnan Agricultural University, Kunming 650201, China
c. College of Agriculture and Biotechnology, Yunnan Agricultural University, Kunming 650201, China

Received:2023-07-13 Online:2024-06-25 Published:2024-07-02

Abstract

Abstract:

To investigate the virus carried by Yunnan’s special variety of ‘Niuwei’ yam and its harmful effects, clarify the effectiveness of viral inhibitors, heat treatment, and low temperature treatment on removing virus from yam, with a view to obtaining better methods for devirusing. The study investigated the incidence of yam virus disease and plant symptoms in ‘Niuwei’ yam, and detected the type of virus using molecular biological detection method, and examined the detoxification effect of different treatments on yam mosaic virus. The results showed that ‘Niuwei’ yam carried yam mosaic virus, and obvious yellowing spots could be seen on its leaves. The incidence rate was 41.67%-43.33%. RT-PCR results showed that treatments with 60 mol·L^-1 moroxydine hydrochloride or 60 mol·L^-1 ribavirin could reduce the survival rate and growth of the yam but effectively remove the yam mosaic virus. High-temperature treatments (12 h at 45 ℃, 24 h at 40 ℃, 24 h at 45 ℃) combined with apical bud culture effectively removed yam mosaic virus. The treatment for 24 h at 40 ℃ significantly reduced the growth of yam, while treatments for 12 h at 45 ℃ and 24 h at 45 ℃ significantly reduced both the growth and survival rate of yam. Low-temperature treatment (24 h at 0 ℃ and 24 h at 5 ℃) combined with apical bud culture significantly reduced the survival rate and growth of yam but were effective in removing the yam mosaic virus. Considering the survival rate, growth, and detoxification effect, 60 mol·L^-1 moroxydine hydrochloride treatment or 40 ℃ high-temperature treatment for 24 h was recommended.

Key words: yam mosaic virus disease, high-temperature, detoxification, stem culture

CLC Number:

S632.1

CHENG Yuan, XU Shengsheng, DUAN Yanbi, XU Xinlei, GUO Fenggen, WANG Shiyu, LONG Wenhong. Investigation on virus disease in ‘Niuwei’ yam and removal of virus with stem culture[J]. Acta Agriculturae Zhejiangensis, 2024, 36(6): 1329-1338.

Figures/Tables 11

References 28

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检测病毒 Detect virus	引物名称 Primer name	序列(5'→3') Sequence (5'→3')	退火温度 Annealing temperature/℃
YMMV	YMMVDetF1 8716	TTCCTCGCATCAAAGCACCAATGAG	59.58
	YMMVDetR1 9254	TGCTGCYTTCATYTGCATGTG	53.66
	YMMVDetR1a9254	TGCCGCTTTCATTTGCATGTG	55.61
JYMV	JYMVF8430	TACGCATGGCTTCTTGGGCAAGC	61.33
	JYMVR9256	TTCCTCGAAGTCCATAGCGTGGC	61.33
	JYMVR9256a	TCCTCTGAAGTCCATAGCGTGGC	61.33
YCNV	YCNMVF604	TTAACGTCATAGTAGCTCTCAGC	55.99
	YCNMVR1269	TAGTCGAGCTGAACCCTAACCAC	59.55
YMOV	YMOVF4146	ATTGTGGTTCATGCAGTTGCAGG	57.77
	YMOVR5056	ATATACAACAAGTGCAACTAACACG	54.66

检测病毒 Detect virus	引物名称 Primer name	序列(5'→3') Sequence (5'→3')	退火温度 Annealing temperature/℃
YMMV	YMMVDetF1 8716	TTCCTCGCATCAAAGCACCAATGAG	59.58
	YMMVDetR1 9254	TGCTGCYTTCATYTGCATGTG	53.66
	YMMVDetR1a9254	TGCCGCTTTCATTTGCATGTG	55.61
JYMV	JYMVF8430	TACGCATGGCTTCTTGGGCAAGC	61.33
	JYMVR9256	TTCCTCGAAGTCCATAGCGTGGC	61.33
	JYMVR9256a	TCCTCTGAAGTCCATAGCGTGGC	61.33
YCNV	YCNMVF604	TTAACGTCATAGTAGCTCTCAGC	55.99
	YCNMVR1269	TAGTCGAGCTGAACCCTAACCAC	59.55
YMOV	YMOVF4146	ATTGTGGTTCATGCAGTTGCAGG	57.77
	YMOVR5056	ATATACAACAAGTGCAACTAACACG	54.66

质量浓度 Mass concentration/ (mg·L^-1)	成活率 Survival rate/%	侧芽萌发数 Number of sprouting lateral buds	侧芽长出数 Number of elongating lateral buds	株高 Plant height/cm	叶色 Leaf color
20	95.53±0.91 a	2.68±0.67 a	1.30±0.98 ab	2.13±0.35 a	绿Green
40	96.63±2.91 a	2.34±0.28 c	1.20±0.05 b	1.87±0.40 a	绿Green
60	70.30±0.95 b	2.14±0.53 d	0.64±0.05 c	1.00±0.26 b	绿Green
0(CK)	94.63±1.24 a	2.59±0.65 ab	1.42±0.53 a	2.23±0.15 a	绿Green

质量浓度 Mass concentration/ (mg·L^-1)	成活率 Survival rate/%	侧芽萌发数 Number of sprouting lateral buds	侧芽长出数 Number of elongating lateral buds	株高 Plant height/cm	叶色 Leaf color
20	95.53±0.91 a	2.68±0.67 a	1.30±0.98 ab	2.13±0.35 a	绿Green
40	96.63±2.91 a	2.34±0.28 c	1.20±0.05 b	1.87±0.40 a	绿Green
60	70.30±0.95 b	2.14±0.53 d	0.64±0.05 c	1.00±0.26 b	绿Green
0(CK)	94.63±1.24 a	2.59±0.65 ab	1.42±0.53 a	2.23±0.15 a	绿Green

质量浓度 Mass concentration/ (mg·L^-1)	成活率 Survival rate/%	侧芽萌发数 Number of sprouting lateral buds	侧芽长出数 Number of elongating lateral buds	株高 Plant height/cm	叶色 Leaf color
20	82.20±3.05 b	1.23±0.53 b	0.42±1.15 b	0.96±0.15 b	绿Green
40	63.57±3.23 c	1.18±0.20 b	0.41±0.58 b	0.50±0.10 c	绿Green
60	55.43±0.75 d	0.87±0.53 c	0.15±0.95 c	0.19±0.79 d	绿Green
0(CK)	94.63±1.24 a	2.59±0.65 a	1.42±0.53 a	2.23±0.15 a	绿Green

Investigation on virus disease in ‘Niuwei’ yam and removal of virus with stem culture

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处理 treatment	成活率 Survival rate/%	侧芽萌发数 Number of sprouting lateral buds	侧芽长出数 Number of elongating lateral buds	株高 Plant height/cm	叶色 Leaf color
40 ℃ 12 h	86.20±2.89 a	2.09±0.97 a	1.16±0.98 a	2.10±0.30 a	绿Green
45 ℃ 12 h	63.00±4.11 b	1.62±0.62 b	0.80±0.53 b	1.46±0.21 b	绿Green
40 ℃ 24 h	81.37±3.25 a	1.96±0.87 b	1.00±0.77 b	1.96±0.06 b	绿Green
45 ℃ 24 h	53.90±3.53 c	1.15±0.25 c	0.43±0.53 c	0.90±0.10 c	嫩绿Light green
对照组Control	87.13±4.59 a	2.16±0.79 a	1.22±0.15 a	2.27±0.15 a	绿Green

处理 Treatment	成活率 Survival rate/%	侧芽萌发数 Number of sprouting lateral buds	侧芽长出数 Number of elongating lateral buds	株高 Plant height/cm	叶色 Leaf color
0 ℃ 12 h	80.83±1.40 b	1.17±1.00 b	8.33±1.77 b	0.68±1.23 b	嫩绿Light green
-5 ℃ 12 h	60.63±2.15 c	1.09±0.08 c	5.00±1.00 c	0.55±0.09 bc	嫩绿Light green
0 ℃ 24 h	55.87±1.79 d	1.05±0.53 c	5.33±0.58 bc	0.45±0.05 c	嫩绿Light green
-5 ℃ 24 h	45.73±0.50 e	1.01±0.33 d	4.03±0.98 d	0.45±0.05 c	嫩绿Light green
对照组Control	87.13±1.15 a	2.16±0.79 a	12.00±0.15 a	2.27±0.15 a	绿Green

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