Determination of arbutin in plant-derived products by solid phase extraction-high performance liquid chromatography

doi:10.3969/j.issn.1004-1524.20240448

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (8): 1776-1784.DOI: 10.3969/j.issn.1004-1524.20240448

• Food Science • Previous Articles Next Articles

Determination of arbutin in plant-derived products by solid phase extraction-high performance liquid chromatography

CHEN Min¹^,²(), ZHANG Qiaoyan², WANG Xiajun², WANG Shunli¹^,^*(), ZHENG Weiran²^,^*()

1. School of Science, Zhejiang Sci-Tech University, Hangzhou 310018, China
2. Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2024-05-22 Online:2025-08-25 Published:2025-09-03
Contact: WANG Shunli,ZHENG Weiran

Abstract

Abstract:

A solid phase extraction(SPE)-high performance liquid chromatography (HPLC) method was developed for the determination of arbutin in plant-derived products ( fruits and Chinese herbal medicines). The samples with high water content and low water content were extracted by water and methanol respectively, purified by HLB solid phase extraction column, and analyzed by high performance liquid chromatography. The results showed that the mass concentration of arbutin had a good linear relationship with the chromatographic peak area in the range of 0.000 5-0.05 mg·mL^-1, the correlation coefficient was 0.999 8. The recovery rates of arbutin at three different spiked levels were 81.33%-97.01%, and the relative standard deviations were 1.39%-3.65% (n=6). The established method was applied to plant-derived products. The content of arbutin in pear was higher, followed by blueberry, raspberry and Bletilla striata. The method has good practicability, accurate results and wide application range. It can be used as a detection method for arbutin in plant-derived products and provide scientific reference for the quality evaluation of plant-derived products.

Key words: arbutin, plant-derived product, solid-phase extraction(SPE), high performance liquid chromatography (HPLC)

CLC Number:

CHEN Min, ZHANG Qiaoyan, WANG Xiajun, WANG Shunli, ZHENG Weiran. Determination of arbutin in plant-derived products by solid phase extraction-high performance liquid chromatography[J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1776-1784.

Figures/Tables 8

References 22

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样品 Sample	加标含量 Added content/ (mg·kg^-1)	回收率 Recovery rate/%						平均回收率 Average recoveries/%	RSD/%
样品 Sample	加标含量 Added content/ (mg·kg^-1)	1	2	3	4	5	6	平均回收率 Average recoveries/%	RSD/%
早酥梨	1	83.27	87.15	89.84	84.19	86.76	82.01	85.54	3.39
Zaosu pear	10	88.51	88.05	83.97	84.12	87.78	85.74	86.36	2.35
	100	93.43	96.13	96.07	95.33	94.27	97.01	95.37	1.39
白及	1	81.33	85.48	82.89	89.12	87.65	82.55	84.84	3.65
Bletilla striata	10	86.17	86.14	84.78	82.22	89.75	82.59	85.28	3.29
	100	92.15	93.86	89.44	91.36	94.84	93.15	92.47	2.08

样品 Sample	加标含量 Added content/ (mg·kg^-1)	回收率 Recovery rate/%						平均回收率 Average recoveries/%	RSD/%
样品 Sample	加标含量 Added content/ (mg·kg^-1)	1	2	3	4	5	6	平均回收率 Average recoveries/%	RSD/%
早酥梨	1	83.27	87.15	89.84	84.19	86.76	82.01	85.54	3.39
Zaosu pear	10	88.51	88.05	83.97	84.12	87.78	85.74	86.36	2.35
	100	93.43	96.13	96.07	95.33	94.27	97.01	95.37	1.39
白及	1	81.33	85.48	82.89	89.12	87.65	82.55	84.84	3.65
Bletilla striata	10	86.17	86.14	84.78	82.22	89.75	82.59	85.28	3.29
	100	92.15	93.86	89.44	91.36	94.84	93.15	92.47	2.08

样品 Sample	称样量 Sample weight/g	提取溶剂 Extraction solvent	提取溶剂体积 Extraction solvent volume/mL	超声时间 Ultrasonic time/min	固相萃取柱 Solid phase extraction	含量 Content/ (mg·kg^-1)	文献 Reference
覆盆子Raspberry	1.00	甲醇Methanol	4	30	HLB	12.92	本文This study
覆盆子Raspberry	1.00	水Water	10	30	无None	ND	[18]
覆盆子Raspberry	1.00	水Water	100	30	无None	ND	[13]
覆盆子Raspberry	1.00	70%甲醇70% Methanol	150	30	HLB	ND	[20]
覆盆子Raspberry	1.00	70%甲醇70% Methanol	50	30	HLB	11.54	[21]
早酥梨Zaosu pear	1.00	水Water	4	20	HLB	87.69	本文This study
早酥梨Zaosu pear	1.00	乙醇Ethanol	4	0	C18	19.69	[15]
早酥梨Zaosu pear	1.00	80%甲醇80% Methanol	10	0	无None	76.85	[22]

样品 Sample	称样量 Sample weight/g	提取溶剂 Extraction solvent	提取溶剂体积 Extraction solvent volume/mL	超声时间 Ultrasonic time/min	固相萃取柱 Solid phase extraction	含量 Content/ (mg·kg^-1)	文献 Reference
覆盆子Raspberry	1.00	甲醇Methanol	4	30	HLB	12.92	本文This study
覆盆子Raspberry	1.00	水Water	10	30	无None	ND	[18]
覆盆子Raspberry	1.00	水Water	100	30	无None	ND	[13]
覆盆子Raspberry	1.00	70%甲醇70% Methanol	150	30	HLB	ND	[20]
覆盆子Raspberry	1.00	70%甲醇70% Methanol	50	30	HLB	11.54	[21]
早酥梨Zaosu pear	1.00	水Water	4	20	HLB	87.69	本文This study
早酥梨Zaosu pear	1.00	乙醇Ethanol	4	0	C18	19.69	[15]
早酥梨Zaosu pear	1.00	80%甲醇80% Methanol	10	0	无None	76.85	[22]

水果Fruit	含量Content/(mg·kg^-1)	中草药Chinese herbal medicine	含量Content/(mg·kg^-1)
苹果Apple	ND	元胡Corydalis yanhusuo	ND
脐橙Navel orange	ND	玄参Scrophularia yunnanensis	ND
葡萄Grape	ND	浙贝母Fritillaria thunbergii	ND
水蜜桃Peach	ND	白芍Paeonia lactiflora	ND
草莓Strawberry	ND	白术Atractylodes macrocephala	ND
石榴Pomegranate	ND	覆盆子Raspberry	12.92
红果参Campanumoea lancifolia	ND	白及Bletilla striata	8.13
早酥梨Zaosu pear	87.69	黄芩Scutellaria baicalensis	ND
蓝莓Blueberry	13.39	铁皮石斛Dendrobium officinale	3.25

Determination of arbutin in plant-derived products by solid phase extraction-high performance liquid chromatography

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t/min	A/%	B/%	流速Flow rate/(mL·min^-1)
0	95	5	0.8
12	95	5	0.8
15	70	30	0.8
17	20	80	0.8
20	95	5	0.8
23	95	5	0.8

t/min	A/%	B/%	流速Flow rate/(mL·min^-1)
0	95	5	0.8
12	95	5	0.8
15	70	30	0.8
17	20	80	0.8
20	95	5	0.8
23	95	5	0.8