Preparation of chitosan-chitosan oligosaccharide composite film and its application in strawberry preservation

doi:10.3969/j.issn.1004-1524.20240787

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (8): 1785-1793.DOI: 10.3969/j.issn.1004-1524.20240787

• Food Science • Previous Articles Next Articles

Preparation of chitosan-chitosan oligosaccharide composite film and its application in strawberry preservation

CHEN Wei¹(), WANG Rongrong¹, JIANG Wenjing², GENG Weisong¹, CHEN Cen¹^,^*()

1. School of Tea & Food Science, Jiangsu Vocational College of Agriculture and Forestry, Jurong 212400, Jiangsu, China
2. College of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, China

Received:2024-09-04 Online:2025-08-25 Published:2025-09-03
Contact: CHEN Cen

Abstract

Abstract:

Chitosan-chitosan oligosaccharide composite films were prepared via the solution casting method by using chitosan as film-forming matrix with addition of chitosan oligosaccharide. The effects of chitosan oligosaccharide addition amounts (3%, 6%, 12% and 24%, based on the weight of chitosan) on the performance of composite films were determined by measuring physicochemical, mechanical and antimicrobial properties. The results indicated that with the increase of chitosan oligosaccharide content in the composite film, the opacity and tensile strength increased, the elongation at break decreased, and the antibacterial effect enhanced. Structural characterization obsevesd by scanning electron microscope (SEM) suggested that chitosan oligosaccharide and chitosan matrix had good compatibility. Compared with the blank control, composite films wrapped as a packaging material for strawberry could reduce the natural decay rate and weight loss rate of strawberry, and decrease the loss of soluble solids and vitamin C. When chitosan oligosaccharide content was 12% of the weight of chitosan, the composite film showed the best preservation effect on strawberry. This study could provide reference for the preparation of chitosan composite film and its application in fruit and vegetable preservation.

Key words: chitosan, chitosan oligosaccharide, strawberry, composite film, preservation

CLC Number:

TS255.3

CHEN Wei, WANG Rongrong, JIANG Wenjing, GENG Weisong, CHEN Cen. Preparation of chitosan-chitosan oligosaccharide composite film and its application in strawberry preservation[J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1785-1793.

Figures/Tables 13

Fig.1 Photographs of appearance of composite films

Table 1 Thickness, chromaticity and opacity of composite films

样品 Sample	膜厚度/mm	L^*	a^*	b^*	ΔE	不透明度 Opacity
ch-cos0	0.067±0.006 a	89.91±0.26 a	-0.68±0.09 c	3.02±0.41 b	5.41±0.18 c	1.78±0.09 c
ch-cos3	0.077±0.006 a	89.45±0.19 a	-0.57±0.07 c	4.06±0.13 b	6.01±0.02 c	1.93±0.14 c
ch-cos6	0.073±0.006 a	88.57±0.30 a	-0.55±0.02 c	5.32±0.76 b	6.80±0.69 c	2.00±0.07 bc
ch-cos12	0.077±0.006 a	85.04±0.83 b	0.76±0.21 b	14.80±1.34 a	16.10±1.41 b	2.26±0.13 ab
ch-cos24	0.070±0.001 a	84.86±0.79 b	1.25±0.13 a	17.06±0.94 a	18.41±0.95 a	2.38±0.15 a

Fig.2 Microstructure of composite films

Fig.3 Tensile strength (TS) and elongation at break (EAB) of composite films Bars marked without the same letters indicate significant (p<0.05) difference within treatments for the same index. The same as in Fig. 5.

Fig.4 Water vapor permeance (WVP) of composite films Bars marked without the same letters indicate significant difference at p<0.05.

Fig.5 Antibacterial effect of composite films

Fig.6 Effect of treatments on decay rate of strawberry Dots marked without the same letters indicate significant (p<0.05) difference at the same time. The same as in Fig. 8.

Fig.7 Appearance of strawberries after storage of 6 days under treatments

Fig.8 Effect of treatments on weight loss rate of strawberry

Fig.9 Effect of treatments on hardness of strawberry Bars marked without the same letters indicate significant (p<0.05) difference within treatments at the same storage time.The same as below.

Fig.10 Effect of treatments on titratable acid content of strawberry

Fig.11 Effect of treatments on soluble solids content of strawberry

Fig.12 Effect of treatments on vitamin C content of strawberry

References 25

[1]	唐甜甜, 解新方, 任雪, 等. 草莓贮藏保鲜技术的研究进展[J]. 食品工业科技, 2020, 41(5): 332-339.
	TANG T T, XIE X F, REN X, et al. Research progress on storage and preservation technology of strawberry[J]. Science and Technology of Food Industry, 2020, 41(5): 332-339. (in Chinese with English abstract)
[2]	LIU B D, ZHANG J H, GUO H G. Research progress of polyvinyl alcohol water-resistant film materials[J]. Membranes, 2022, 12(3): 347.
[3]	LI X, LI F H, ZHANG X, et al. Interaction mechanisms of edible film ingredients and their effects on food quality[J]. Current Research in Food Science, 2024, 8: 100696.
[4]	SARFRAZ M H, HAYAT S, SIDDIQUE M H, et al. Chitosan based coatings and films: a perspective on antimicrobial, antioxidant, and intelligent food packaging[J]. Progress in Organic Coatings, 2024, 188: 108235.
[5]	石洁, 王雪嵘, 徐朝阳. 食品包装用壳聚糖薄膜的研究进展[J]. 包装工程, 2022, 43(3): 42-50.
	SHI J, WANG X R, XU Z Y. Research progress of chitosan films for food packaging[J]. Packaging Engineering, 2022, 43(3): 42-50. (in Chinese with English abstract)
[6]	HERAS M, HUANG C C, CHANG C W, et al. Trends in chitosan-based films and coatings: a systematic review of the incorporated biopreservatives, biological properties, and nanotechnology applications in meat preservation[J]. Food Packaging and Shelf Life, 2024, 42: 101259.
[7]	LI B, CUI J C, XU T T, et al. Advances in the preparation, characterization, and biological functions of chitosan oligosaccharide derivatives: a review[J]. Carbohydrate Polymers, 2024, 332: 121914.
[8]	YUAN X B, ZHENG J P, JIAO S M, et al. A review on the preparation of chitosan oligosaccharides and application to human health, animal husbandry and agricultural production[J]. Carbohydrate Polymers, 2019, 220: 60-70.
[9]	亓伟杰, 连子腾, 代世成, 等. 不同浓度大豆分离蛋白对壳聚糖-纳米纤维可食膜结构及性能的影响[J]. 中国食品学报, 2023, 23(6): 267-275.
	QI W J, LIAN Z T, DAI S C, et al. Effects of different concentrations of soybean protein isolate on the structure and properties of chitosan-nanofiber edible membrane[J]. Journal of Chinese Institute of Food Science and Technology, 2023, 23(6): 267-275. (in Chinese with English abstract)
[10]	颜田田, 戚勃, 杨贤庆, 等. 增塑剂对卡拉胶可食用膜性能的影响[J]. 食品与发酵工业, 2019, 45(23): 97-102.
	YAN T T, QI B, YANG X Q, et al. Effects of plasticizers on properties of carrageenan edible films[J]. Food and Fermentation Industries, 2019, 45(23): 97-102. (in Chinese with English abstract)
[11]	罗爱国, 赵琪, 马建华, 等. 藻蓝蛋白-壳聚糖复合膜的制备及性能表征[J]. 食品工业科技, 2020, 41(23): 25-29.
	LUO A G, ZHAO Q, MA J H, et al. Preparation and characterization of phycocyanin-chitosan composite films[J]. Science and Technology of Food Industry, 2020, 41(23): 25-29. (in Chinese with English abstract)
[12]	YAN J, HE S C, CHEN L L, et al. Characterization, antioxidant and antibacterial activities of gelatin-chitosan edible coated films added with Cyclocarya paliurus flavonoids[J]. International Journal of Biological Macromolecules, 2023, 253: 127664.
[13]	BI F Y, YONG H M, LIU J, et al. Development and characterization of chitosan and D-α-tocopheryl polyethylene glycol 1000 succinate composite films containing different flavones[J]. Food Packaging and Shelf Life, 2020, 25: 100531.
[14]	张爱武, 宋亭, 张丽媛, 等. 玉米秸秆纳米纤维素-淀粉膜的制备工艺优化及性能分析[J]. 食品工业科技, 2022, 43(11): 252-259.
	ZHANG A W, SONG T, ZHANG L Y, et al. Preparation process optimization and property analysis of nanocellulose-starch film based on corn stalk[J]. Science and Technology of Food Industry, 2022, 43(11): 252-259. (in Chinese with English abstract)
[15]	张馨月, 杨禹诚, 段皓月, 等. 氮掺杂碳量子点-壳聚糖复合膜的制备及对草莓的保鲜效果[J]. 浙江农业学报, 2023, 35(12): 2935-2943.
	ZHANG X Y, YANG Y C, DUAN H Y, et al. Preparation of nitrogen-doped carbon quantum dots-chitosan composite film and its preservation effect on strawberry[J]. Acta Agriculturae Zhejiangensis, 2023, 35(12): 2935-2943. (in Chinese with English abstract)
[16]	舒畅, 刘帮迪, 张万立, 等. 近冰温冷藏对‘金冠’苹果贮藏品质的影响[J]. 食品科学, 2020, 41(1): 244-251.
	SHU C, LIU B D, ZHANG W L, et al. Improving postharvest quality of apple (Malus×domestica Borkh. cv. Golden Delicious) fruit by storage at near freezing temperature[J]. Food Science, 2020, 41(1): 244-251. (in Chinese with English abstract)
[17]	曹建康, 姜微波, 赵玉梅. 果蔬采后生理生化实验指导[M]. 北京: 中国轻工业出版社, 2007: 39-41.
[18]	唐森, 李孟玲, 陈显玲, 等. 紫苏精油壳聚糖复合膜的制备及其对鲜切雪莲果的保鲜效果研究[J]. 粮食与油脂, 2023, 36(9): 61-65.
	TANG S, LI M L, CHEN X L, et al. Preparation of perilla essential oil chitosan composite film and its preservation effect on fresh-cut yacon[J]. Cereals & Oils, 2023, 36(9): 61-65. (in Chinese with English abstract)
[19]	DONG J L, YU D W, YU Z J, et al. Thermally-induced crosslinking altering the properties of chitosan films: structure, physicochemical characteristics and antioxidant activity[J]. Food Packaging and Shelf Life, 2022, 34: 100948.
[20]	陶海燕, 赵丽凤, 陈刚, 等. 富含苹果皮根皮素壳聚糖-聚乙烯醇抗氧化保鲜膜的研制[J]. 华中师范大学学报(自然科学版), 2022, 56(4): 648-655.
	TAO H Y, ZHAO L F, CHEN G, et al. Preparation of chitosan-PVA composite membranes containing phloretin and used as antioxidant film[J]. Journal of Central China Normal University(Natural Sciences), 2022, 56(4): 648-655. (in Chinese)
[21]	李凯. 基于壳聚糖/γ-PGA基可降解复合保鲜膜的制备及保鲜效果的研究[D]. 成都: 成都大学, 2023.
	LI K. Study on preparation and preservation effect of CS/γ-PGA based degradable composite plastic wrap[D]. Chengdu: Chengdu University, 2023. (in Chinese with English abstract)
[22]	FERNÁNDEZ-DE CASTRO L, MENGÍBAR M, SÁNCHEZ Á, et al. Films of chitosan and chitosan-oligosaccharide neutralized and thermally treated: effects on its antibacterial and other activities[J]. LWT, 2016, 73: 368-374.
[23]	刘永旭, 张大伟. 壳聚糖/聚乙烯醇/壳寡糖抑菌纳米纤维膜的制备和性能研究[J]. 生物质化学工程, 2020, 54(4): 30-36.
	LIU Y X, ZHANG D W. Preparation and properties of bacteriostatic chitosan/PVA/chitosan oligosaccharide nanofibrous membranes[J]. Biomass Chemical Engineering, 2020, 54(4): 30-36. (in Chinese with English abstract)
[24]	王鑫, 周卓, 岳振歌, 等. PVA-甜玉米芯多糖纳米银复合薄膜的制备及草莓保鲜应用[J]. 精细化工, 2024, 41(9): 2018-2030.
	WANG X, ZHOU Z, YUE Z G, et al. Preparation of PVA-sweet corncob polysaccharide nano-silver composite film and its application in strawberry preservation[J]. Fine Chemicals, 2024, 41(9): 2018-2030. (in Chinese with English abstract)
[25]	ZHU J Y, FANG Y J, WAKISAKA M, et al. Fabrication of flexible chitosan film reinforced with pulping by-product lignosulfonates for cherry-tomato preservation[J]. Food Chemistry: X, 2024, 21: 101181.

Preparation of chitosan-chitosan oligosaccharide composite film and its application in strawberry preservation

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 13

References 25

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Fan, ZHANG Xiaofeng, LI Jianying, CHEN Xiaoming, LIU Wei, ZHOU Fan, MENG Qinghui. Evaluation of sperm cryopreservation effectiveness for largemouth bass(Micropterus salmoides) based on computer-assisted sperm analysis [J]. Acta Agriculturae Zhejiangensis, 2025, 37(7): 1417-1423.
[2]	MA Xian, YOU Yuwei, KANG Juan, WANG Guoqin, ZHENG Rui, SU Jianyu, YUE Sijun. Pathogen identification of post-harvest rotten Lycium barbarum and screening of natural fungicides [J]. Acta Agriculturae Zhejiangensis, 2025, 37(6): 1327-1335.
[3]	SHEN Lan, YANG Xiaofang, ZHANG Guofang. Identification and fungicide sensitivity of the pathogen causing root rot on strawberry [J]. Acta Agriculturae Zhejiangensis, 2025, 37(2): 417-425.
[4]	ZONG Zihao, HE Dingsheng, NIU Ben, HUANG Jun, FANG Xiangjun, WU Weijie, CHEN Hangjun, GAO Haiyan. Effect of different hollow packaging on storage and transportation quality of bayberry harvested in rainy days [J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 196-204.
[5]	WANG Shiyao, XIN Zhenzhong, WANG Lian, WANG Yu. Preparation of beta@chitosan fresh-keeping coating and its effect on the quality of Taigu pear jujube [J]. Acta Agriculturae Zhejiangensis, 2023, 35(9): 2212-2221.
[6]	YANG Qing, LIU Shenghong, HUANG Erbin, DU Rongyu, WANG Fang, DENG Jia. Carboxymethyl chitosan-induced transcriptome WRKY genes analysis and resistance-related gene mining in grapefruit fruit [J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 598-614.
[7]	ZHANG Xinyue, YANG Yucheng, DUAN Haoyue, ZHOU Yangjie, HUANG Jiayang, CHEN Ziyue, JIANG Jie, CHEN Shujuan. Preparation of nitrogen-doped carbon quantum dots-chitosan composite film and its preservation effect on strawberry [J]. Acta Agriculturae Zhejiangensis, 2023, 35(12): 2935-2943.
[8]	YANG Xiaofang, LI Yunduan, SUN Yunfan, LI Shaojia, MIAO Lixiang, ZHANG Yuchao, JIANG Guihua. Influence of substrate cultivation and soil cultivation on sucrose and citric acid accumulation of Yuexin strawberry [J]. Acta Agriculturae Zhejiangensis, 2022, 34(7): 1423-1430.
[9]	GUO Xuesong, TIAN Libo, SHANG Sang. Effect of malonic acid treatment on postharvest storage quality of cowpea [J]. Acta Agriculturae Zhejiangensis, 2022, 34(7): 1529-1536.
[10]	CAI Jiye, FANG Xiangjun, HAN Yanchao, DING Yuting, CHEN Hangjun, WU Weijie, GAO Haiyan. Effect controlled atmosphere storage on postharvest preservation of Dongkui bayberry [J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 352-359.
[11]	WANG Lifang, YE Liang, XIE Zhongwen, DANG Xiangli. Antibacterial activity of tea antimicrobial peptide extraction and its effect on preservation of chilled meat [J]. Acta Agriculturae Zhejiangensis, 2022, 34(10): 2268-2276.
[12]	TIAN Yutan, MA Lu, LIU Juna, LI Dongdong, TAO Yingmei, ZHAO Xiaolu, MA Yanan, SUN Shaoyi, LIU Dunhua. Application of edible composite film of wine residue polyphenol-chitosan-CMC during storage of boiled mutton [J]. Acta Agriculturae Zhejiangensis, 2021, 33(6): 1095-1103.
[13]	LI Qingbin, QIN Benben, LI Yingying, FAN Kaifeng, YANG Dong, CHEN Lei, LIU Kun. Effects of continuous rain and sunless weather on microclimate, strawberry growth and quality in greenhouse [J]. Acta Agriculturae Zhejiangensis, 2021, 33(5): 831-839.
[14]	WU Jian, WANG Jiangong, CHU Weixiong. Effect of natamycin treatment on grape quality in e-commerce logistics process [J]. Acta Agriculturae Zhejiangensis, 2021, 33(5): 916-922.
[15]	CHANG Huiqing, XU Fujin, PAN Yajie. Passivation effects of calcium carbonate with/without chitosan on chromium pollution in calcareous soil [J]. , 2020, 32(9): 1665-1671.