Isolation and sequence analysis of anthocyanidin synthase gene BaANS from Brassica albograbra

›› 2010, Vol. 22 ›› Issue (2): 0-166.

• 论文 •

Isolation and sequence analysis of anthocyanidin synthase gene BaANS from Brassica albograbra

ZHAO Rong-rong;JIANG Ming*;HE Cai-ming;ZHU Ya-qin;ZHOU Min

College of Life Science, Taizhou University, Linhai 317000, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-03-25 Published:2010-03-25

Abstract

Abstract: Degenerate primer pairs were designed according to known anthocyanidin synthase gene sequences in NCBI database. The gene, designated BaANS, was isolated from leaf genomic DNA and cDNA of Chinese kale (Brassica albograbra), respectively. Genomic DNA of BaANS gene is 1 369 bp in length with one intro of 292 bp. Complete coding sequence of BaANS is 1 077 bp in length encoding 358 amino acids. The sequence has been submitted to NCBI with accession number of GU170203. Sequence comparison with Matthiola incana, B. oleracea var. capitata, B. juncea, B. rapa ssp. campestris and Arabidopsis thaliana showed that they shared high homology．

Key words: Chinese kale (Brassica albograbra), anthocyanidin synthase, sequence analysis

ZHAO Rong-rong;JIANG Ming*;HE Cai-ming;ZHU Ya-qin;ZHOU Min. Isolation and sequence analysis of anthocyanidin synthase gene BaANS from Brassica albograbra[J]. , 2010, 22(2): 0-166.

[1]	LIU Kai, FENG Xiaoyu, MA Hengjia, XIE Nan. Complete sequence and gene organization of mitochondrial genome of Megalobrama terminalis from Qiantang River [J]. , 2020, 32(9): 1591-1608.
[2]	NI Zheng, CHEN Liu, HUA Jionggang, YE Weicheng, YUN Tao, ZHU Yinchu, ZHANG Cun. Detection of leukaemia virus antigen and analysis of GP85 gene sequence in local chicken breeds in Zhejiang Province [J]. , 2020, 32(8): 1357-1362.
[3]	WANG Weike, SONG Jiling, LU Na, YUAN Weidong, YAN Jing, CHEN Guanping. Cloning and expression of the PpFBD1 involved in primordium formation of Pleurotus pulmonarius [J]. , 2020, 32(1): 93-97.
[4]	DEXI Cuomu, WANG Yin, YANG Zexiao, YAO Xueping, LUO Yan, LIAO Changyu, ZHANG Pengfei, JIANG Dike, XIANG Mingyuan, JIANG Ruijiao, SONG Yong. Epidemiological investigation of porcine epidemic diarrhea virus and sequence analysis of ORF3 and S1 genes in Sichuan from 2016 to 2018 [J]. , 2019, 31(9): 1423-1428.
[5]	YI Keke, YIN Wenqi, ZHOU Yuancheng, JIANG Jinzhen, ZHANG Baiyu, LI Zhongyin, YAN Qigui. Isolation and identification of 4 strains of porcine pseudorabies virus and analysis of main virulence genes [J]. , 2019, 31(9): 1429-1436.
[6]	LIU Jialin, LIU Shili, JIANG Wenping, CHENG Shun, CHI Meili, ZHENG Jianbo, JIA Yongyi, ZHAO Jinliang, YIN Shaowu, GU Zhimin. Cloning and bioinformatics analysis of GH gene and its flanking region in Odontobutis potamophila [J]. , 2019, 31(9): 1461-1470.
[7]	WANG Yuanhong, LU Zhimin, ZHANG Xueqi, LIU Zimin, HU Zihui, YANG Kankan, LIU Hongmei, PAN Ling, PENG Kaisong, LI Yongdong, WANG Yong. Isolation, identification and phylogenetic analysis on gp85 gene of subgroup J avian leukosis virus [J]. , 2019, 31(5): 709-715.
[8]	BA Shaobo, SHI Lin, LI Qunjing, LIU Zhengkui, CHEN Lin, WANG Xiaodu, SONG Houhui. Isolation, identification and molecullar epidemiological analysis of 17-ZJ-HZ strain of porcine reproductive and respiratory syndrome virus [J]. , 2018, 30(8): 1303-1311.
[9]	LIU Shili, JIA Yongyi, JIANG Wenping, CHI Meili, CHENG Shun, ZHAO Jinliang, GU Zhimin, FU Jianjun. Cloning and sequence analysis of growth hormone gene and its flanking region in topmouth culter (Culter alburnus Basilewsky) [J]. , 2017, 29(8): 1281-1289.
[10]	WANG Yan, LIU Yao, LIANG Chenggang, CHEN Qingqing, SHI Taoxiong, CHEN Qijiao, MENG Ziye, CHEN Qingfu. Sequence analysis of ABA-insensitive gene of common buckwheat, Fagopyrum esculentum [J]. , 2017, 29(2): 186-192.
[11]	GAO Jianfeng, LU Zengkui, MA Youji, LI Taotao1, ZHAO Xingxu. Cloning, sequence analysis and tissue expression of MUSTN1 gene in sheep (Ovis aires) [J]. , 2017, 29(10): 1661-1668.
[12]	ZHANG Yang, WANG Meiqing, MU Chunyu, YAO Wencheng, YANG Yaozong, BIAN Youqing, CHEN Guohong. Analysis of mitochondrial CO Ⅰ gene bar code and its upstream tRNA sequence determination [J]. , 2017, 29(1): 51-57.
[13]	ZOU Li， YANG Yuan\\|yi， SUN Ting\\|ting， WANG Shi\\|xin， CUI Rong， LI Jing\\|ying. Isolation and identification of wild Lepista sordida by ITS gene sequence [J]. , 2016, 28(2): 264-.
[14]	LI Wei1，#，Zhang Fei\\|yan1，#，SONG Ting\\|ting1，GU Xiao\\|xiao1，LIU Hong\\|lu1，PAN Da\\|min1，HAN Shu\\|bing1，PAN Kang\\|cheng1，2，*. Cloning and sequence analysis of IGF\\|I gene in Tibetan pig and Yanan pig#br# [J]. , 2015, 27(8): 1324-.
[15]	QIU Haiping;MAO Xueqin;JIANG Hua;WANG Yanli;SUN Guochang;*. Diversity research on Rhizoctonia solani isolated from Zhejiang Province using sequence analysis of 58S rDNAITS interval [J]. , 2014, 26(3): 0-702707.

Isolation and sequence analysis of anthocyanidin synthase gene BaANS from Brassica albograbra

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments