Isolation and RT-PCR identification of a new-type Duck hepatitis virus

›› 2010, Vol. 22 ›› Issue (5): 596-598.

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Isolation and RT-PCR identification of a new-type Duck hepatitis virus

MA Xiu-li;ZHAO Li-na;XIA Xue-mei;WU Jing;LIN Shu-qian;JIANG Yi-fei

¹Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Jinan 250023, China；²Shandong Agricultural University, Tai'an 271018, China; ³Department of Environment and Life Sciences, Kaili College, Kaili 556011, China

Received:1900-01-01 Revised:1900-01-01 Online:2010-09-25 Published:2010-09-25

Abstract

Abstract: One stain of Duck hepatitis virus was isolated from Shandong Province and named as JFX08. The results of serum neutralization test showed that there was no cross-protection occurred in strain JFX08 and Duck hepatitis virus typeⅠ(DHV-Ⅰ). Clinical symptom and pathological lesion could be reproduced in 3-day ducklings challenged by the isolated strain. A pair of primers was designed and synthesized according to the sequence of DHV and a reverse transcriptase polymerase chain reaction method was developed for strain JFX08. The identity analysis of nucleotide sequence between strain JFX08 and N-DHV from Korean (genotype C) showed the similarity was 93.2%-94.0%. However, the nucleotide sequence identity between strain JFX08 and DHV-Ⅰ was much lower. Phylogenetic and evolutionary analysis showed that the genetic distance between the isolated strain JFX08 and N-DHV from Korean was the nearest, indicating JFX08 belonged to genotype C, which was a different branch from genotype A and B．

Key words: DHV new\|type, isolation, RT-PCR, identification

MA Xiu-li;ZHAO Li-na;XIA Xue-mei;WU Jing;LIN Shu-qian;JIANG Yi-fei. Isolation and RT-PCR identification of a new-type Duck hepatitis virus[J]. , 2010, 22(5): 596-598.

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Isolation and RT-PCR identification of a new-type Duck hepatitis virus

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