Abstract: One stain of Duck hepatitis virus was isolated from Shandong Province and named as JFX08. The results of serum neutralization test showed that there was no cross-protection occurred in strain JFX08 and Duck hepatitis virus typeⅠ(DHV-Ⅰ). Clinical symptom and pathological lesion could be reproduced in 3-day ducklings challenged by the isolated strain. A pair of primers was designed and synthesized according to the sequence of DHV and a reverse transcriptase polymerase chain reaction method was developed for strain JFX08. The identity analysis of nucleotide sequence between strain JFX08 and N-DHV from Korean (genotype C) showed the similarity was 93.2%-94.0%. However, the nucleotide sequence identity between strain JFX08 and DHV-Ⅰ was much lower. Phylogenetic and evolutionary analysis showed that the genetic distance between the isolated strain JFX08 and N-DHV from Korean was the nearest, indicating JFX08 belonged to genotype C, which was a different branch from genotype A and B．

Key words: DHV new\|type, isolation, RT-PCR, identification