Abstract: Using PCR amplification, sequencing and assembling, the complete mitochondrial genome of Shaoxing duck (Anas platyrhychos) was obtained. The full sequence was acquired from 15 primers designed according to close species mitochondrial genome. The entire mitochondrial genome of Shaoxing duck was 16 604 bp in length, which contained 37 genes (13 protein coding genes, 2 rRNA, 22 tRNA) and a non-coding control region (D-loop). The composition of the nucleotides A, T, G, C was 29.20%, 22.19%, 15.78%, 32.83%, respectively. The gene composition and sequence arrangement was similar to other birds, indicating that mitochondrial DNA of bird was more conservative in evolution．

Key words: Shaoxing duck, mitochondrial genome, sequence analysis