烟草钾离子通道基因NtTPK的克隆及表达分析

doi:10.3969/j.issn.1004-1524.2017.03.03

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (3): 366-372.DOI: 10.3969/j.issn.1004-1524.2017.03.03

烟草钾离子通道基因NtTPK的克隆及表达分析

许力, 黄路平, 鲁黎明, 李立芹^*

四川农业大学农学院,四川成都 625014

收稿日期:2016-10-28 出版日期:2017-03-20 发布日期:2017-03-31
通讯作者: 李立芹,E-mail:liliqin88@163.com
作者简介:许力(1992—),女,四川中江人,硕士研究生,主要从事植物分子生物学研究。E-mail:892334388@qq.com
基金资助:
植物生理学与生物化学国家重点实验室开放课题(SKLPPBKF 1505, SKLPPBKF 1506)

Cloning and expression analysis of potassium channel NtTPK gene in tobacco

XU Li, HUANG Luping, LU Liming, LI Liqin^*

College of Agronomy, Sichuan Agricultural University, Chengdu 625014, China

Received:2016-10-28 Online:2017-03-20 Published:2017-03-31

摘要/Abstract

摘要： 采用同源克隆的方法对烟草品种K326中的一个钾离子通道基因NtTPK进行了研究,该基因序列全长1 317 bp,编码438个氨基酸。实时荧光定量PCR结果表明,该基因在茎中的表达量最高;在低钾处理24 h后该基因表达量最低;200 mmol·L^-1 NaCl处理24 h后该基因表达量最高;在烟草打顶7 d后,该基因在叶和根中的表达量达到最高。这些结果为进一步研究该基因的功能提供了一定理论基础。

关键词: 烟草, 钾离子通道, NtTPK, 生物信息学, 基因表达

Abstract: A new potassium channel gene NtTPK was cloned from cultivar K326 using homologous cloning strategy. Sequences analysis showed this gene contained 1 317 bp and coded 438 amino acid residues. Quantitative real-time PCR results showed that the highest expression level of this gene was in the stem; and the lowest expression level emerged after 24 h under low potassium treatment; the gene expression reached its peak level after 24 h under 200 mmol·L^-1 NaCl treatment; and 7 d after topping, the gene expression showed the highest level in leaves and roots of tobacco. These results provide theoretical bases for further study of the function of potassium channel NtTPK gene.

Key words: tobacco, potassium channel, NtTPK, bioinformatics, gene expression

中图分类号:

S572

许力, 黄路平, 鲁黎明, 李立芹. 烟草钾离子通道基因NtTPK的克隆及表达分析[J]. 浙江农业学报, 2017, 29(3): 366-372.

XU Li, HUANG Luping, LU Liming, LI Liqin. Cloning and expression analysis of potassium channel NtTPK gene in tobacco[J]. , 2017, 29(3): 366-372.

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烟草钾离子通道基因NtTPK的克隆及表达分析

Cloning and expression analysis of potassium channel NtTPK gene in tobacco

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