正交设计优化中药类植物提取物组方

doi:10.3969/j.issn.1004-1524.2021.12.09

浙江农业学报 ›› 2021, Vol. 33 ›› Issue (12): 2295-2303.DOI: 10.3969/j.issn.1004-1524.2021.12.09

正交设计优化中药类植物提取物组方

陶新(), 吴宛澐, 孙雨晴, 门小明, 徐子伟^*()

浙江省农业科学院畜牧兽医研究所,浙江杭州 310021

收稿日期:2021-06-28 出版日期:2021-12-25 发布日期:2022-01-10
通讯作者: 徐子伟
作者简介:* 徐子伟, E-mail: xzwfyz@sina.com
陶新(1976—),女,山东聊城人,博士,研究员,主要从事动物营养与健康养殖研究。E-mail: xindragon@126.com
基金资助:
浙江省重点研发计划(2018C02035);浙江省重点研发计划(2021C02007);浙江省农业(畜禽)新品种选育重大科技专项(2016C02054);现代农业产业技术体系建设专项(CARS-35)

Optimization of Chinese medicine plant extract compounds with orthogonal design

TAO Xin(), WU Wanyun, SUN Yuqing, MEN Xiaoming, XU Ziwei^*()

Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-06-28 Online:2021-12-25 Published:2022-01-10
Contact: XU Ziwei

摘要/Abstract

摘要：

为制备可替代饲用抗生素的中药类复合植物提取物,首先以抑菌率、抗炎率和抗氧化IC₅₀值为评价指标,从19种中药类植物中遴选出石榴皮、桂枝、厚朴、青蒿和鸡血藤5种原料作为复合提取物组分;然后采用L₁₆(4⁵)正交试验设计优化组分不同配比,对试验结果进行极差分析,获得分别具有最佳抑菌(F_ab)、抗炎(F_ai)和抗氧化(F_ao)功能的3个组方,其组分中厚朴、鸡血藤、青蒿、桂枝和石榴皮的比例依次为7∶13∶6∶9∶1、7∶13∶4∶1∶1和3∶13∶4∶1∶7。验证试验结果显示:F_ab最小抑菌浓度为125 mg·mL^-1,F_ai抗炎IC₅₀值为0.144 mg·mL^-1,F_ao抗氧化IC₅₀值为0.222 mg·mL^-1。通过以上研究,获得兼具抑菌、抗炎和抗氧化功能的复合植物提取物最优组方3个。

关键词: 中药, 正交设计, 植物提取物组方, 抑菌, 抗炎, 抗氧化

Abstract:

To prepare Chinese medicine plant extracts replacing feed antibiotics. Firstly, Grana Pericarpium, Cinnamomi Ramulus, Magnoliae officinalis cortex, Artemisiae annuae herba, Spatholobi caulis were sieved from 19 Chinese medicine plants as materials of extract compounds by examining antibacterial rate, inhibitory rateanti-inflammatory rate, and antioxidant IC₅₀ value as evaluating index. Secondly, proportions of five materials were optimized by a L₁₆ (4 ⁵) orthogonal experiment,by range analysis of results, three extract compounds with the strongest antibacterial (F_ab), anti-inflammatory (F_ai) and antioxidant (F_ao) activities were obtained. These material ratios of Magnoliae officinalis cortex, Spatholobi caulis, Artemisiae annuae herba, Cinnamomi Ramulus and Grana Pericarpium were 7∶13∶6∶9∶1 in F_ab, 7∶13∶4∶1∶1 in F_ai, and 3∶13∶4∶1∶7 in F_ao, respectively. Finally, the verifying results showed that the minimum inhibitory concentration of F_ab was 125 mg·mL^-1, the anti-inflammatory IC₅₀ value of F_ai was 0.144 mg·mL^-1, and the antioxidant IC₅₀ value of F_ao was 0.222 mg·mL^-1. In summary, the present study obtained the best three Chinese medicine extract compounds with stronger antibacterial, anti-inflammatory and antioxidant effects, respectively.

Key words: Chinese medicine, orthogonal design, plant extract compounds, antibacterial, anti-inflammatory, antioxidant

中图分类号:

S853.7

陶新, 吴宛澐, 孙雨晴, 门小明, 徐子伟. 正交设计优化中药类植物提取物组方[J]. 浙江农业学报, 2021, 33(12): 2295-2303.

TAO Xin, WU Wanyun, SUN Yuqing, MEN Xiaoming, XU Ziwei. Optimization of Chinese medicine plant extract compounds with orthogonal design[J]. Acta Agriculturae Zhejiangensis, 2021, 33(12): 2295-2303.

图/表 9

参考文献 19

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水平 Level	因素 Factors
水平 Level	A厚朴 Magnoliae officinalis cortex	B鸡血藤 Spatholobi caulis	C青蒿 Artemisiae annuae herba	D桂枝 Cinnamomi Ramulus	E石榴皮 Grana Pericarpium
1	1	7	4	1	1
2	3	9	6	3	3
3	5	11	8	6	5
4	7	13	10	9	7

水平 Level	因素 Factors
水平 Level	A厚朴 Magnoliae officinalis cortex	B鸡血藤 Spatholobi caulis	C青蒿 Artemisiae annuae herba	D桂枝 Cinnamomi Ramulus	E石榴皮 Grana Pericarpium
1	1	7	4	1	1
2	3	9	6	3	3
3	5	11	8	6	5
4	7	13	10	9	7

试验号 Number	因素Factors					评价指标Evaluation index
试验号 Number	A	B	C	D	E	抑菌率 Antibacterial rate/%	抗炎率 Anti-inflammatoryrate/%	抗氧化IC₅₀值 Antioxidant IC₅₀value/(mg·mL^-1)
1	1	1	1	1	1	34.53±5.988	86.90±2.755	0.817±0.039
2	1	2	2	2	2	33.73±2.762	78.49±2.425	0.822±0.043
3	1	3	3	3	3	9.370±1.931	69.54±1.091	0.682±0.027
4	1	4	4	4	4	36.53±4.651	75.06±1.655	0.395±0.026
5	2	1	2	3	4	26.95±4.262	57.16±1.137	0.293±0.018
6	2	2	1	4	3	31.74±4.383	73.06±3.190	0.272±0.018
7	2	3	4	1	2	17.82±3.835	79.89±0.825	0.481±0.039
8	2	4	3	2	1	38.52±9.606	94.32±0.689	0.695±0.021
9	3	1	3	4	2	59.08±3.609	72.19±0.852	0.726±0.035
10	3	2	4	3	1	79.44±8.643	91.77±0.369	2.011±0.056
11	3	3	1	2	4	8.908±3.602	81.67±2.451	0.335±0.028
12	3	4	2	1	3	79.64±7.162	92.81±0.995	0.266±0.031
13	4	1	4	2	3	60.48±5.215	73.47±0.730	0.288±0.012
14	4	2	3	1	4	28.54±1.521	79.68±0.705	0.245±0.032
15	4	3	2	4	1	81.04±5.463	93.47±0.521	1.090±0.037
16	4	4	1	3	2	71.06±5.624	97.53±1.587	0.722±0.029

试验号 Number	因素Factors					评价指标Evaluation index
试验号 Number	A	B	C	D	E	抑菌率 Antibacterial rate/%	抗炎率 Anti-inflammatoryrate/%	抗氧化IC₅₀值 Antioxidant IC₅₀value/(mg·mL^-1)
1	1	1	1	1	1	34.53±5.988	86.90±2.755	0.817±0.039
2	1	2	2	2	2	33.73±2.762	78.49±2.425	0.822±0.043
3	1	3	3	3	3	9.370±1.931	69.54±1.091	0.682±0.027
4	1	4	4	4	4	36.53±4.651	75.06±1.655	0.395±0.026
5	2	1	2	3	4	26.95±4.262	57.16±1.137	0.293±0.018
6	2	2	1	4	3	31.74±4.383	73.06±3.190	0.272±0.018
7	2	3	4	1	2	17.82±3.835	79.89±0.825	0.481±0.039
8	2	4	3	2	1	38.52±9.606	94.32±0.689	0.695±0.021
9	3	1	3	4	2	59.08±3.609	72.19±0.852	0.726±0.035
10	3	2	4	3	1	79.44±8.643	91.77±0.369	2.011±0.056
11	3	3	1	2	4	8.908±3.602	81.67±2.451	0.335±0.028
12	3	4	2	1	3	79.64±7.162	92.81±0.995	0.266±0.031
13	4	1	4	2	3	60.48±5.215	73.47±0.730	0.288±0.012
14	4	2	3	1	4	28.54±1.521	79.68±0.705	0.245±0.032
15	4	3	2	4	1	81.04±5.463	93.47±0.521	1.090±0.037
16	4	4	1	3	2	71.06±5.624	97.53±1.587	0.722±0.029

因素 Factor	抑菌率 Antibacterial rate						抗炎率 Anti-inflammatory rate						抗氧化IC50值 Antioxidant IC50 value
因素 Factor	k₁/ %	k₂/ %	k₃/ %	k₄/ %	R/ %	最优水平 The best level	k₁/ %	k₂/ %	k₃/ %	k₄/ %	R/ %	最优水平 The best level	k₁/ (mg· mL^-1)	k₂/ (mg· mL^-1)	k₃/ (mg· mL^-1)	k₄/ (mg· mL^-1)	R/ (mg· mL^-1)	最优水平 The best level
A	28.54	28.76	56.77	60.28	31.74	4	77.50	76.11	84.61	86.79	9.929	4	0.679	0.435	0.835	0.587	0.4	2
B	45.26	43.36	29.29	56.44	27.15	4	72.43	80.75	81.14	89.93	17.50	4	0.531	0.838	0.647	0.519	0.319	4
C	36.56	55.34	33.88	48.57	21.46	2	84.79	80.48	78.93	80.05	5.857	1	0.536	0.618	0.587	0.794	0.258	1
D	40.13	35.41	46.71	52.1	16.69	4	84.82	81.99	79.00	78.45	6.375	1	0.452	0.535	0.927	0.621	0.475	1
E	58.38	45.42	45.31	25.23	33.15	1	91.62	82.03	77.22	73.39	18.22	1	1.153	0.688	0.377	0.317	0.836	4

正交设计优化中药类植物提取物组方

Optimization of Chinese medicine plant extract compounds with orthogonal design

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参考文献 19

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变异来源	自由度	均方	F值	P值
Sources of variation	Degree of freedom	Mean square	F value	P value
抑菌率 Antibacterial rate
A	3	3 594.26	126.06	<0.001
B	3	894.22	31.36	<0.001
C	3	1 226.28	43.01	<0.001
D	3	1 438.99	50.47	<0.001
E	3	2 834.09	99.40	<0.001
误差Error	32	28.51	—	—
抗炎率 Anti-inflammatory rate
A	3	298.33	114.46	<0.001
B	3	574.09	220.26	<0.001
C	3	79.14	30.36	<0.001
D	3	65.26	25.04	<0.001
E	3	577.70	221.64	<0.001
误差Error	32	2.606	—	—
抗氧化IC₅₀值 Antioxidant IC₅₀ value
A	3	0.336	318.64	<0.001
B	3	0.114	108.29	<0.001
C	3	0.150	142.26	<0.001
D	3	0.586	555.44	<0.001
E	3	1.458	1 383.4	<0.001
误差Error	32	0.001	—	—

因素 Factor	水平 Level	评价指标Evaluation index
因素 Factor	水平 Level	抑菌率 Antibacterial rate%	抗炎率 Anti-inflammatory rate%	抗氧化IC50值 Antioxidant IC50 value/(mg·mL^-1)
A	1	28.54±1.541 b	77.50±0.446 b	0.679±0.009 b
	2	28.76±1.541 b	76.11±0.446 c	0.435±0.009 b
	3	56.77±1.541 a	87.11±0.446 a	0.834±0.009 d
	4	60.28±1.541 a	86.04±0.446 a	0.586±0.009 c
B	1	40.26±1.748 b	72.08±0.528 c	0.577±0.011 c
	2	40.13±1.748 b	80.49±0.528 b	0.753±0.011 a
	3	37.51±1.748 b	81.75±0.528 b	0.685±0.011 b
	4	56.44±1.541 a	89.93±0.466 a	0.519±0.009 d
C	1	36.56±1.541 c	84.79±0.466 a	0.536±0.009 c
	2	63.57±2.101 a	81.09±0.635 b	0.656±0.013 b
	3	30.65±2.101 d	78.67±0.635 c	0.503±0.013 c
	4	43.57±2.101 b	79.70±0.635 bc	0.840±0.013 a
D	1	36.90±1.748 c	84.56±0.528 a	0.368±0.011 d
	2	30.41±1.748 d	81.64±0.528 b	0.581±0.011 c
	3	46.71±1.541 b	79.00±0.466 c	0.927±0.009 a
	4	60.33±1.748 a	79.05±0.528 c	0.659±0.011 b
E	1	66.61±1.748 a	92.22±0.528 a	1.191±0.011 a
	2	45.42±1.541 b	82.03±0.466 b	0.688±0.009 b
	3	40.31±1.748 c	76.87±0.528 c	0.423±0.011 c
	4	22.00±1.748 d	73.14±0.528 d	0.233±0.011 d

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