基质栽培与土壤栽培对越心草莓蔗糖和柠檬酸积累的影响

doi:10.3969/j.issn.1004-1524.2022.07.09

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (7): 1423-1430.DOI: 10.3969/j.issn.1004-1524.2022.07.09

基质栽培与土壤栽培对越心草莓蔗糖和柠檬酸积累的影响

杨肖芳¹(), 李云端², 孙云帆², 李绍佳², 苗立祥¹, 张豫超¹, 蒋桂华¹^,^*()

1.浙江省农业科学院园艺研究所,浙江杭州 310021
2.浙江大学农业与生物技术学院,浙江杭州 310058

收稿日期:2021-09-16 出版日期:2022-07-25 发布日期:2022-07-26
通讯作者: 蒋桂华
作者简介:* 蒋桂华,E-mail: jgh2004267@sina.com
杨肖芳(1981—),女,浙江诸暨人,助理研究员,学士,主要从事草莓品种选育与栽培技术研究。E-mail: 228021786@qq.com
基金资助:
浙江省自然科学基金(LGN20C150008);浙江省科技厅重点研发计划(2020C02001);浙江省团队特派员项目(2019R05A58C01);国家重点研发计划(2020YFD1000105);宁波市科技创新2025重大专项(2019B10021);浙江省育种项目(2021C02066-7)

Influence of substrate cultivation and soil cultivation on sucrose and citric acid accumulation of Yuexin strawberry

YANG Xiaofang¹(), LI Yunduan², SUN Yunfan², LI Shaojia², MIAO Lixiang¹, ZHANG Yuchao¹, JIANG Guihua¹^,^*()

1. Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310058, China

Received:2021-09-16 Online:2022-07-25 Published:2022-07-26
Contact: JIANG Guihua

摘要/Abstract

摘要：

为了解基质栽培和土壤栽培条件下草莓果实品质形成的差异,探究其影响规律,以越心草莓为材料,测定果实不同发育时期的蔗糖和柠檬酸含量,以及相关基因的表达量。结果显示,土壤栽培条件下蔗糖和柠檬酸含量高于基质栽培,其中,蔗糖积累的差异主要体现在草莓果实的尖部,柠檬酸积累的差异在果实尖部和基部。FaSPS2基因是土壤栽培和基质栽培蔗糖积累差异的关键基因,与蔗糖积累呈正相关。FaACO1、FaACO3基因的表达量与柠檬酸的积累呈负相关,基质栽培中FaACO1、FaACO3的表达量显著高于土壤栽培,导致基质栽培的成熟草莓果实中的柠檬酸含量低于土壤栽培。因此,FaSPS2、FaACO1和FaACO3基因在越心草莓蔗糖和柠檬酸含量的积累中发挥了重要作用,土壤栽培较基质栽培可能更有利于越心草莓蔗糖和柠檬酸含量积累。

关键词: 基质栽培, 土壤栽培, 草莓, 蔗糖, 柠檬酸

Abstract:

In order to study the difference of strawberry fruit quality formation under substrate cultivation and soil cultivation, and explore its influence law, the contents of sucrose and citric acid and the expression of related genes in different development stages of strawberry were measured. The results showed that contents of sucrose and citric acid in soil culture were higher than those in substrate culture. The difference of sucrose accumulation was mainly reflected in the tip of strawberry fruit, and the difference of citric acid accumulation was in the tip and base of strawberry fruit. FaSPS2 gene was the key gene for the difference of sucrose accumulation between soil culture and substrate culture, which was positively correlated with sucrose accumulation. The expression of FaACO1 and FaACO3 genes were negatively correlated with the accumulation of citric acid. The expression of FaACO1 and FaACO3 in matrix culture were significantly higher than those in soil culture, resulting in the citric acid content of mature strawberry fruit in matrix culture was lower than that in soil culture. Therefore, FaSPS2, FaACO1 and FaACO3 genes played important role in the accumulation of sucrose and citric acid content of Yuexin strawberry. Soil cultivation might be more conducive to the accumulation of sucrose and citric acid content of Yuexin strawberry than substrate cultivation.

Key words: substrate cultivation, soil cultivation, strawberry, sucrose, citric acid

中图分类号:

S668.4

杨肖芳, 李云端, 孙云帆, 李绍佳, 苗立祥, 张豫超, 蒋桂华. 基质栽培与土壤栽培对越心草莓蔗糖和柠檬酸积累的影响[J]. 浙江农业学报, 2022, 34(7): 1423-1430.

YANG Xiaofang, LI Yunduan, SUN Yunfan, LI Shaojia, MIAO Lixiang, ZHANG Yuchao, JIANG Guihua. Influence of substrate cultivation and soil cultivation on sucrose and citric acid accumulation of Yuexin strawberry[J]. Acta Agriculturae Zhejiangensis, 2022, 34(7): 1423-1430.

图/表 7

表1 实时荧光定量PCR引物序列

Table 1 Primers for real-time quantitative PCR

基因 Gene	编码蛋白 Encoding protein	正向引物 Forward primer (5'→3')	反向引物 Reverse primer (5'→3')
FaSUS1	SUS	CCCTGATTCTGACCTTTACTGG	GATGATGAAGTCGGTGTGGTT
FaSUS2	SUS	AAGAAGAACTTGTAGATGGGAGGTC	ATAATTGAAGAAAGGTGGCGGT
FaSPS1	SPS	GAGCCTTGAATGTCCCAATGT	ATCTCCTGTCTGGTGCTGGTT
FaSPS2	SPS	ACTGTGCAAAGTTGGCGTCTT	GATCATCGCAATCCACGGCTA
FaSPS3	SPS	CGTAGATTGGAGTTATGGAGAGC	CGAATGATGTAAGAACCACTGC
FaCS1	CS	AATCTCTGCGGCGGTTTGAC	TGGCTTTGATGGTTCCTTGTTC
FaCS2	CS	CATCCCACTGCCGATGTCTT	GACTCTGATGGTTGGCTGCT
FaCS4 FaACO1 FaACO3	CS ACO ACO	AATGAGAGGAATGACGGGGT CCCCAAAACAATCCATCATCC TTGGTTGGTATGGGTATTATTCC	TAATCTGGAACTTTGGCACGA TTCCTCACCAGTCAATCCAAGA GCTTTATCTCACTGATGTTGCTAGG
FaRIB413	RIB	ACCGTTGATTCGCACAATTGGTCATCG	TACTGCGGGTCGGCAATCGGACG

图1 基质栽培和土壤栽培不同发育时期的草莓果实蔗糖含量 A, 果实尖部;B, 果实基部。W,白果期;T,转色期;IR,半红期;R,全红期。下同。

Fig.1 Sucrose content of strawberry cultivated under substrate and soil during different fruit developmental periods A, Tip; B, Base. W, White stage; T, Turning stage; IR, Intermediate red stage; R, Full red stage. The same as below.

图2 基质栽培和土壤栽培不同发育时期的草莓果实柠檬酸含量 A,尖部;B,基部。

Fig.2 Citric acid content of strawberry cultivated under substrate and soil during different fruit developmental periods A, Tip; B, Base.

图3 基质栽培和土壤栽培条件下蔗糖合成代谢相关基因的表达模式

Fig.3 Expression pattern of genes related to sucrose anabolism under substrate and soil cultivation

图4 蔗糖含量与FaSPS2基因相对表达量的相关性分析

Fig.4 Correlation analysis between sucrose content and relative expression of FaSPS2

图5 基质栽培和土壤栽培条件下柠檬酸降解相关基因的表达模式

Fig.5 Expression patterns of genes related to citric acid degradation under substrate culture and soil culture

图6 柠檬酸含量与FaACO1基因和FaACO3基因相对表达量的相关性分析

Fig.6 Correlation analysis between citric acid content and relative expression of FaACO1 and FaACO3

参考文献 20

[1]	LI M J, FENG F J, CHENG L L. Expression patterns of genes involved in sugar metabolism and accumulation during apple fruit development[J]. PLoS One, 2012, 7(3): e33055.
[2]	TANASE K, YAMAKI S. Purification and characterization of two sucrose synthase isoforms from Japanese pear fruit[J]. Plant and Cell Physiology, 2000, 41(4): 408-414. DOI URL
[3]	ZHANG N, JIANG J, YANG Y L, et al. Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit[J]. Journal of Zhejiang University-Science B (Biomedicine & Biotechnology), 2015, 16(10): 845-856. DOI URL
[4]	DENG W, LUO K M, LI Z G, et al. Overexpression of Citrus junos mitochondrial citrate synthase gene in Nicotiana benthamiana confers aluminum tolerance[J]. Planta, 2009, 230(2): 355-365. DOI URL
[5]	MORGAN M J, OSORIO S, GEHL B, et al. Metabolic engineering of tomato fruit organic acid content guided by biochemical analysis of an introgression line[J]. Plant Physiology, 2012, 161(1): 397-407. DOI URL
[6]	张豫超, 杨肖芳, 苗立祥, 等. 优质抗病草莓新品种: ‘越心’的选育[J]. 果树学报, 2015, 32(6): 1294-1296, 996.
	ZHANG Y C, YANG X F, MIAO L X, et al. ‘Yuexin’, a new strawberry cultivar with high quality and disease resistance[J]. Journal of Fruit Science, 2015, 32(6): 1294-1296, 996. (in Chinese with English abstract)
[7]	ZHANG Y Y, YIN X R, XIAO Y W, et al. An ethylene response factor-MYB transcription complex regulates furaneol biosynthesis by activating quinone oxidoreductase expression in strawberry[J]. Plant Physiology, 2018, 178(1): 189-201. DOI URL
[8]	LI S J, YIN X R, WANG W L, et al. Citrus CitNAC62 cooperates with CitWRKY1 to participate in citric acid degradation via up-regulation of CitAco3[J]. Journal of Experimental Botany, 2017, 68(13): 3419-3426. DOI URL
[9]	CHANG S J, PURYEAR J, CAIRNEY J. A simple and efficient method for isolating RNA from pine trees[J]. Plant Molecular Biology Reporter, 1993, 11(2): 113-116. DOI URL
[10]	JUNG S, LEE T, CHENG C H, et al. 15 years of GDR: new data and functionality in the Genome Database for Rosaceae[J]. Nucleic Acids Research, 2019, 47(D1): D1137-D1145.
[11]	AMIL-RUIZ F, GARRIDO-GALA J, BLANCO-PORTALES R, et al. Identification and validation of reference genes for transcript normalization in strawberry (Fragaria×ananassa) defense responses[J]. PLoS One, 2013, 8(8): e70603.
[12]	施羊林, 陈小文, 许庆广, 等. 不同栽培介质及EC值对温室草莓生长的影响[J]. 山西农业大学学报(自然科学版), 2016, 36(8): 562-566.
	SHI Y L, CHEN X W, XU Q G, et al. Effects of different growing media and EC for greenhouse grown strawberries[J]. Journal of Shanxi Agricultural University (Natural Science Edition), 2016, 36(8): 562-566. (in Chinese with English abstract)
[13]	向发云, 曾祥国, 张鹏, 等. “晶瑶”草莓基质栽培与有土栽培生产性状比较[J]. 湖北农业科学, 2011, 50(23): 4858-4860, 4899.
	XIANG F Y, ZENG X G, ZHANG P, et al. Comparison on substrate culture and soil culture of ‘Jingyao’ strawberry in plastic house[J]. Hubei Agricultural Sciences, 2011, 50(23): 4858-4860, 4899. (in Chinese with English abstract)
[14]	谢鸣, 张上隆, 陈俊伟, 等. 基质培对草莓糖酸和某些生理特性的影响[J]. 果树学报, 2004, 21(6): 560-564.
	XIE M, ZHANG S L, CHEN J W, et al. Effect of substrate culture on sugar and acid content and some physiological characteristics of strawberry fruit[J]. Journal of Fruit Science, 2004, 21(6): 560-564. (in Chinese with English abstract)
[15]	邓胜艺, 张佳芮, 姚磊. 基质栽培与土壤栽培罗马花椰菜品质产量的比较研究[J]. 园艺与种苗, 2020, 40(6): 13-14.
	DENG S Y, ZHANG J R, YAO L. Comparative study on quality and production of Roman broccoli cultivated with substrate and soil[J]. Horticulture & Seed, 2020, 40(6): 13-14. (in Chinese with English abstract)
[16]	魏韩英. 海岛草莓基质栽培试验[J]. 农业与技术, 2017, 37(8): 48-49.
	WEI H Y. Culture experiment of island strawberry with substrate[J]. Agriculture and Technology, 2017, 37(8): 48-49. (in Chinese)
[17]	刘中良, 高昕, 张艳艳, 等. 基质栽培与土壤栽培番茄品质产量的比较研究[J]. 江苏农业科学, 2020, 48(1): 124-127.
	LIU Z L, GAO X, ZHANG Y Y, et al. Comparative study on quality and yield of tomato under substrate cultivation and soil cultivation[J]. Jiangsu Agricultural Sciences, 2020, 48(1): 124-127. (in Chinese)
[18]	MIAO L X, ZHANG Y C, YANG X F, et al. Fruit quality, antioxidant capacity, related genes, and enzyme activities in strawberry (Fragaria×ananassa) grown under colored plastic films[J]. HortScience, 2017, 52(9): 1241-1250. DOI URL
[19]	RUAN Y L, JIN Y, YANG Y J, et al. Sugar input, metabolism, and signaling mediated by invertase: roles in development, yield potential, and response to drought and heat[J]. Molecular Plant, 2010, 3(6): 942-955. DOI URL
[20]	IANNETTA P P M, ESCOBAR N M, ROSS H A, et al. Identification, cloning and expression analysis of strawberry (Fragaria×ananassa) mitochondrial citrate synthase and mitochondrial malate dehydrogenase[J]. Physiologia Plantarum, 2004, 121(1): 15-26. DOI URL

基质栽培与土壤栽培对越心草莓蔗糖和柠檬酸积累的影响

Influence of substrate cultivation and soil cultivation on sucrose and citric acid accumulation of Yuexin strawberry

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