浙江农业学报 ›› 2023, Vol. 35 ›› Issue (1): 103-111.DOI: 10.3969/j.issn.1004-1524.2023.01.11

• 园艺科学 • 上一篇    下一篇

不同品种荷花耐盐碱性评价及鉴定指标筛选

刘艺平1,2(), 张一琪1, 苏少文1, 刘红利1,2, 贺丹1,2, 孔德政1,2,*()   

  1. 1.河南农业大学 风景园林与艺术学院,河南 郑州 450000
    2.河南省优质花卉蔬菜种苗工程研究中心,河南 郑州 450000
  • 收稿日期:2021-08-05 出版日期:2023-01-25 发布日期:2023-02-21
  • 通讯作者: *孔德政,E-mail: Kdz217@163.com
  • 作者简介:刘艺平(1997—),女,河南温县人,博士,副教授,研究方向为风景园林植物资源应用。E-mail:Lyp_163@163.com
  • 基金资助:
    河南省高等学校重点研究项目(21A220003);国家自然科学基金(31600568);河南农业大学科技创新基金(KJCX2017C01)

Evaluation of salt-alkali tolerance of different lotus varieties and screening of identification indexes

LIU Yiping1,2(), ZHANG Yiqi1, SU Shaowen1, LIU Hongli1,2, HE Dan1,2, KONG Dezheng1,2,*()   

  1. 1. College of Landscape Architecture and Art, Henan Agricultural University, Zhengzhou 450000, China
    2. Henan Provincial Engineering Research Center of High-Quality Flowers and Vegetables Seedling, Zhengzhou 450000, China
  • Received:2021-08-05 Online:2023-01-25 Published:2023-02-21

摘要:

为研究不同荷花(Nelumbo nucifera)品种在混合盐碱胁迫下的耐受程度,筛选抗性品种,采用盆栽法,以20个荷花品种为试验材料,对其进行100 mmol·L-1(NaCl∶NaHCO3=2∶1)混合盐碱胁迫处理,7 d后测量株高、叶面积、光合色素、叶绿素荧光参数、丙二醛含量、抗氧化物酶活性及渗透调节物质含量,通过耐盐碱系数、相关性分析、主成分分析、隶属函数法、聚类分析等方法对20种荷花耐盐碱性进行综合评价。结果表明:不同荷花品种单项指标的耐盐碱系数变异系数差异较大,除超氧化物歧化酶活性和过氧化物酶活性外,其余单项指标的耐盐碱系数均存在显著或极显著相关性,利用主成分分析将12个单项指标转换为5个综合指标,各综合指标贡献率分别为36.175%、18.709%、13.470%、11.379%、6.610%,累计贡献率达到86.344%。以耐盐碱性综合评价值(D值)为依据进行聚类分析,将20个荷花品种分为4类,第一类为强耐盐碱型,共计4个荷花品种,第二类为耐盐碱型,共计5个荷花品种,第三类为不耐盐碱型,共计8个荷花品种,第四类为敏感型,共计3个荷花品种。通过逐步回归分析建立荷花的耐盐碱性最优评价模型,D=0.453+0.148X1+0.122X2-0.122X7-0.033X8+0.093X11+0.037X12(R2=0.992),估计精度95.45%以上,并筛选出株高、叶面积、丙二醛含量、脯氨酸含量、可溶性糖含量和可溶性蛋白含量为荷花耐盐碱性评价指标,可以在相同的条件下,通过测定这6项指标,确定荷花的耐盐碱性强弱。

关键词: 荷花, 盐碱胁迫, 耐盐碱性, 综合评价, 鉴定指标

Abstract:

In order to study the tolerance of different lotus varieties to saline-alkali stress and to screen resistant varieties, using pot culture method, 20 lotus varieties were used as experimental materials, and 100 mmol·L-1 (NaCl∶NaHCO3=2∶1) hybrid saline-alkali stress treatment, 7 d after measuring the plant height, leaf area, photosynthetic pigments, chlorophyll fluorescence parameters, content of malondialdehyde, activity of antioxidant enzyme and content of osmotic regulatory substances, the saline-alkali tolerance of 20 lotus species was evaluated comprehensively by means of saline-alkali tolerance coefficient, correlation analysis, principal component analysis, membership function method and cluster analysis. The results showed that there were significant differences in the variation coefficients of saline-alkali tolerance among different lotus varieties. Except superoxide dismutase activity and peroxidase activity, the saline-alkali coefficients of other single indexes were significantly or extremely significantly correlated. The 12 single indexes were converted into 5 comprehensive indexes by using principal component analysis. The contribution rates of each comprehensive index were 36.175%, 18.709%, 13.470%, 11.379%, 6.610%, and the cumulative contribution rate reached 86.344%. With saline resistance value (D value) based on clustering analysis, 20 lotus varieties could be divided into four categories, first, there was strong salt-tolerant type, a total of four lotus varieties, and the second category of salt-tolerant type, a total of five lotus varieties, and the third category was not salt-tolerant type, a total of eight lotus varieties, the fourth category for sensitive type, a total of three varieties of lotus. The optimal evaluation model of salinity and alkali tolerance of lotus was established by stepwise regression analysis, D=0.453+0.148X1+0.122X2-0.122X7-0.033X8+0.093X11+0.037X12(R2=0.992), and the estimation accuracy was above 95.45%. Plant height, leaf area, malondialdehyde, proline, soluble sugar and soluble protein were selected as evaluation indexes of the saline-alkali tolerance of lotus. The saline-alkali tolerance of lotus could be determined by measuring these 5 indexes under the same conditions.

Key words: Nelumbo nucifera, saline-alkali stress, saline-alkali resistance, comprehensive evaluation, appraisal indicators

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