仔猪红黄痢二联灭活疫苗与同类制品对比研究

doi:10.3969/j.issn.1004-1524.20240914

浙江农业学报 ›› 2025, Vol. 37 ›› Issue (11): 2258-2264.DOI: 10.3969/j.issn.1004-1524.20240914

仔猪红黄痢二联灭活疫苗与同类制品对比研究

刘泽文(), 袁芳艳, 刘威, 高婷, 杨克礼, 郭锐, 李畅, 吴琼, 朱佳佳, 田永祥, 周丹娜()

湖北省农业科学院畜牧兽医研究所,农业农村部畜禽细菌病防治制剂创制重点实验室,畜禽病原微生物学湖北省重点实验室,湖北武汉 430064

收稿日期:2024-10-25 出版日期:2025-11-25 发布日期:2025-12-08
作者简介:刘泽文(1969—),男,副研究员,从事家畜疾病诊断与预防研究工作。E-mail: liuzwen2004@sina.com
通讯作者: ^*周丹娜,E-mail:zdn_66@126.com
基金资助:
湖北省农业科技创新中心项目(2019-620-000-001-017);湖北省自然科学基金重点类项目(群体)(2021CFA019);湖北省技术创新专项重大项目(2021ABA005)

Comparative study on inactivated vaccines and similar products against Escherichia coli and Clostridium perfringens

LIU Zewen(), YUAN Fangyan, LIU Wei, GAO Ting, YANG Keli, GUO Rui, LI Chang, WU Qiong, ZHU Jiajia, TIAN Yongxiang, ZHOU Danna()

Key Laboratory of Prevention and Control Agents for Animal Bacteriosis, Ministry of Agriculture and Rural Affairs, Hubei Provincial Key Laboratory of Livestock and Poultry of Pathogen Microbiology, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064,China

Received:2024-10-25 Online:2025-11-25 Published:2025-12-08

摘要/Abstract

摘要：

为了比较作者团队研制的仔猪大肠埃希菌病、C型产气荚膜梭菌病二联灭活疫苗(仔猪红黄痢二联灭活疫苗,以下简称二联苗)与同类二联疫苗及各单苗的安全性和免疫效果,用作者团队研制的仔猪大肠埃希菌病、C型产气荚膜梭菌病二联灭活疫苗分别与国内兽用生物制品企业生产的仔猪大肠埃希菌病三价灭活疫苗、仔猪红痢灭活疫苗和国外某公司生产的同类二联灭活疫苗,在同等条件下进行了性状比较、无菌检验及安全性和免疫保护效果等的对比试验,结果显示,二联苗、国内各单苗及进口二联疫苗的性状和无菌检验均合格,各疫苗对小鼠和断奶仔猪均安全,二联苗对大肠埃希菌各菌株及C型产气荚膜梭菌的攻毒保护率达到80%~100%,与各单苗和同类进口疫苗的免疫效力没有明显差异。结果表明,作者团队研制的仔猪大肠埃希菌病、C型产气荚膜梭菌病二联灭活疫苗质量不低于各单苗和同类二联疫苗。

关键词: 大肠埃希菌, 产气荚膜梭菌, 灭活疫苗, 同类制品, 安全性试验, 效力试验

Abstract:

In order to compare the safety and immune efficacy of the dual inactivated vaccine for piglet Escherichia coli disease and Clostridium perfringens(bivalent vaccine) developed by the author team with similar dual vaccine and individual vaccines, the inactivated vaccine in our laboratory was compared with the trivalent inactivated vaccine against Escherichia coli and the inactivated vaccine against C-type Clostridium perfringens produced by domestic biopharmaceutical companies, as well as the combined inactivated vaccine produced by a foreign company. Comparative experiments were conducted on traits, sterility testing, safety, and immune protection effects under the same conditions. The results showed that the inactivated vaccines were qualified in terms of characteristics and sterility compared with domestic and imported vaccines, and all vaccines were safe for mice and weaned piglets. The protection rate of the inactivated vaccine against various strains of Escherichia coli and C-type Clostridium perfringens achieved 80%-100%, with no significant difference in efficacy compared with various single vaccines and imported vaccines of the same type. The comparison results showed that the quality of the inactivated vaccine developed by author team is not lower than that of each monovalent vaccine, trivalent vaccine, and similar imported vaccine.

Key words: Escherichia coli, C-type Clostridium perfringens, inactivated vaccine, similar vaccine, safety test, immune protection test

中图分类号:

刘泽文, 袁芳艳, 刘威, 高婷, 杨克礼, 郭锐, 李畅, 吴琼, 朱佳佳, 田永祥, 周丹娜. 仔猪红黄痢二联灭活疫苗与同类制品对比研究[J]. 浙江农业学报, 2025, 37(11): 2258-2264.

LIU Zewen, YUAN Fangyan, LIU Wei, GAO Ting, YANG Keli, GUO Rui, LI Chang, WU Qiong, ZHU Jiajia, TIAN Yongxiang, ZHOU Danna. Comparative study on inactivated vaccines and similar products against Escherichia coli and Clostridium perfringens[J]. Acta Agriculturae Zhejiangensis, 2025, 37(11): 2258-2264.

图/表 5

参考文献 27

[1]	JEFFERY J Z, LOCKE A K, ALEJANDRO R, 等. 猪病学[M].赵德明,张中秋,周向梅,等译. 10版. 北京: 中国农业大学出版社, 2014.
[2]	刘泽文, 袁芳艳, 刘威, 等. 仔猪大肠杆菌病、C型产气荚膜梭菌病二联灭活疫苗的研制与应用[J]. 动物医学进展, 2018, 39(6): 24-28.
	LIU Z W, YUAN F Y, LIU W, et al. Development and application of bivalent inactivated vaccine against E.coli and C-type Clostridium perfringens in piglets[J]. Progress in Veterinary Medicine, 2018, 39(6): 24-28. (in Chinese with English abstract)
[3]	刘泽文, 田永祥, 袁芳艳, 等. 仔猪大肠杆菌纤毛抗原反向间接血凝检测方法的建立与应用[J]. 华中农业大学学报, 2017, 36(3): 69-73.
	LIU Z W, TIAN Y X, YUAN F Y, et al. Development and application of a reverse indirect hemoagglutination method for detecting piglet E.coli pili antigen[J]. Journal of Huazhong Agricultural University, 2017, 36(3): 69-73. (in Chinese with English abstract)
[4]	中国兽药典委员会. 中华人民共和国兽药典[M]. 北京: 中国农业出版社, 2015.
[5]	ESFANDIARI P, AMANI J, ALI IMANI FOULADI A, et al. Rapid and specific polymerase chain reaction-enzyme linked immunosorbent assay for detection of Escherichia coli LT toxin from clinical isolates[J]. Archives of Clinical Infectious Diseases, 2016, 12(1): archcid36261p.
[6]	薛舒文. 广西某猪场猪大肠杆菌分离鉴定、耐药性分析及联合用药方案筛选[D]. 武汉: 华中农业大学, 2023.
	XUE S W. Isolation identification, drug resistance analysis and selection of drug combination of swine Escherichia coli from a pig farm in Guangxi[D]. Wuhan: Huazhong Agricultural University, 2023. (in Chinese with English abstract)
[7]	高海慧, 张明艳, 汪洁, 等. 腹泻绵羔羊源大肠杆菌耐药性及LEE毒力岛部分相关基因的检测[J]. 黑龙江畜牧兽医, 2023(6): 89-92.
	GAO H H, ZHANG M Y, WANG J, et al. Detection of drug resistance in diarrheic lamb-derived Escherichia coli and some LEE virulence islands-related genes[J]. Heilongjiang Animal Science and Veterinary Medicine, 2023(6): 89-92. (in Chinese with English abstract)
[8]	史唯地, 康立超, 刘彩霞, 等. 犊牛源肠外致病性大肠杆菌分离鉴定、致病性及耐药性分析[J]. 中国畜牧兽医, 2023, 50(12): 5022-5031.
	SHI W D, KANG L C, LIU C X, et al. Isolation identification, pathogenicity and drug resistance analysis of extraintestinal pathogenic Escherichia coli from calves[J]. China Animal Husbandry & Veterinary Medicine, 2023, 50(12): 5022-5031. (in Chinese)
[9]	苏中华, 陈建春, 央珍, 等. 西藏牦牛腹泻性大肠杆菌的分离鉴定[J]. 甘肃畜牧兽医, 2023, 53(4): 74-79.
	SU Z H, CHEN J C, YANG Z, et al. Isolation and identification of diarrhea Escherichia coli from yaks in Xizang[J]. Gansu Animal Husbandry and Veterinary Medicine, 2023, 53(4): 74-79. (in Chinese)
[10]	张自强, 谢辉, 张倩文, 等. 河南地区兔腹泻源性大肠杆菌分离鉴定及其耐药性分析[J]. 家畜生态学报, 2023, 44(9): 66-71.
	ZHANG Z Q, XIE H, ZHANG Q W, et al. Isolation, identification and drug resistance analysis of Escherichia coli from rabbit diarrhea in Henan area[J]. Journal of Domestic Animal Ecology, 2023, 44(9): 66-71. (in Chinese with English abstract)
[11]	JUNG K, SAIF L J, WANG Q H. Porcine epidemic diarrhea virus (PEDV): an update on etiology, transmission, pathogenesis, and prevention and control[J]. Virus Research, 2020, 286: 198045.
[12]	房海. 大肠埃希氏菌[M]. 石家庄: 河北科学技术出版社, 1997.
[13]	CANIBE N, HØJBERG O, KONGSTED H, et al. Review on preventive measures to reduce post-weaning diarrhoea in piglets[J]. Animals, 2022, 12(19): 2585.
[14]	杨丽, 孙小美, 金梅林. 猪源产气荚膜梭菌分离鉴定、耐药性分析及益生菌对产气荚膜梭菌的抑制作用[J]. 养殖与饲料, 2023, 22(3): 10-17.
	YANG L, SUN X M, JIN M L. Isolation identification, drug resistance analysis and inhibition effect of probiotics against Clostridium perfringens from pigs[J]. Animals Breeding and Feed, 2023, 22(3): 10-17. (in Chinese with English abstract)
[15]	沈达. 江苏省部分规模化猪场肠道病原菌的临床检测及大肠杆菌的部分特性研究[D]. 扬州: 扬州大学, 2021.
	SHEN D. Clinical detection of enteric pathogens in some large-scale pig farms in Jiangsu and research of some characteristics of Escherichia coli[D]. Yangzhou: Yangzhou University, 2021. (in Chinese with English abstract)
[16]	刘红燕, 郭亚男, 王建东, 等. 宁夏某牛场致泌乳奶牛急性死亡的产气荚膜梭菌分离鉴定及其药敏试验[J]. 家畜生态学报, 2024, 45(6): 65-70.
	LIU H Y, GUO Y N, WANG J D, et al. Identification and antibiotic susceptibility test of Clostridium perfringens causing acute death of dairy cows in a cattle farm of Ningxia Hui autonomous region[J]. Journal of Domestic Animal Ecology, 2024, 45(6): 65-70. (in Chinese with English abstract)
[17]	刘伟, 刘煜, 张汇宁, 等. 兔产气荚膜梭菌病的诊断及致病菌株生物学特性试验[J]. 中国兽医杂志, 2020, 56(7): 24-30, 148.
	LIU W, LIU Y, ZHANG H N, et al. The research of diagnosing Clostridium perfringens disease in rabbits and biological characteristics of isolated strains[J]. Chinese Journal of Veterinary Medicine, 2020, 56(7): 24-30, 148. (in Chinese with English abstract)
[18]	黄紫贝, 罗健雅, 赵以恒, 等. 肉鸡源产气荚膜梭菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2024, 51(3): 1298-1307.
	HUANG Z B, LUO J Y, ZHAO Y H, et al. Isolation, identification and drug resistance analysis of Clostridium perfringens from broiler chickens[J]. China Animal Husbandry & Veterinary Medicine, 2024, 51(3): 1298-1307. (in Chinese with English abstract)
[19]	王怀禹. 产气荚膜梭菌引起仔猪腹泻的研究进展[J]. 猪业科学, 2022, 39(1): 28-30.
	WANG H Y. Research progress on diarrhea caused by Clostridium perfringens in piglets[J]. Swine Industry Science, 2022, 39(1): 28-30. (in Chinese)
[20]	杨德鸿, 张鹏, 于沛欣, 等. 苏北地区规模化猪场产肠毒素大肠杆菌的分离鉴定及灭活疫苗效果评估[J]. 微生物学报, 2020, 60(2): 261-272.
	YANG D H, ZHANG P, YU P X, et al. Isolation, identification and evaluation of inactivated vaccine of enterotoxigenic Escherichia coli(ETEC) in large-scale pig farms in northern Jiangsu[J]. Acta Microbiologica Sinica, 2020, 60(2): 261-272. (in Chinese with English abstract)
[21]	陈晓锋, 楼平儿, 华坚青, 等. 两种仔猪大肠杆菌疫苗免疫效果试验[J]. 浙江畜牧兽医, 2003, 28(2): 5.
	CHEN X F, LOU P E, HUA J Q, et al. Experiment on immunity of two E. coli bacterins from piglets[J]. Zhejiang Journal of Animal Science and Veterinary Medicine, 2003, 28(2): 5. (in Chinese with English abstract)
[22]	王东, 张思雨, 丁雅文, 等. 产气荚膜梭菌毒素疫苗研究进展[J]. 中国感染控制杂志, 2024, 23(7): 910-917.
	WANG D, ZHANG S Y, DING Y W, et al. Research progress in Clostridium perfringens toxin vaccines[J]. Chinese Journal of Infection Control, 2024, 23(7): 910-917. (in Chinese with English abstract)
[23]	吕林芬, 庞胜美, 李保良, 等. 仔猪产肠毒素大肠杆菌疫苗的研究进展[J]. 河南农业科学, 2023, 52(5): 1-8.
	LYU L F, PANG S M, LI B L, et al. Research progress of enterotoxigenic Escherichia coli vaccine for piglets[J]. Journal of Henan Agricultural Sciences, 2023, 52(5): 1-8. (in Chinese with English abstract)
[24]	刘泽文, 曹宏云, 杨前平, 等. C型产气荚膜梭菌不同菌株交叉免疫保护力研究[J]. 湖北农业科学, 2010, 49(9): 2184-2185.
	LIU Z W, CAO H Y, YANG Q P, et al. Immunity mutual protection research of different C-type Clostridium wechii[J]. Hubei Agricultural Sciences, 2010, 49(9): 2184-2185. (in Chinese with English abstract)
[25]	李莹慧, 张迪, 张兆天, 等. 毕赤酵母提高产肠毒素大肠杆菌灭活疫苗免疫保护效果的研究[J]. 中国预防兽医学报, 2022, 44(12): 1324-1331.
	LI Y H, ZHANG D, ZHANG Z T, et al. A study on improving the immune protection effects of ETEC inactivated vaccine by Pichia pastoris[J]. Chinese Journal of Preventive Veterinary Medicine, 2022, 44(12): 1324-1331. (in Chinese with English abstract)
[26]	李生庆, 张西云, 胡国元, 等. C型产气荚膜梭菌产α毒素能力分析及免疫原性试验研究[J]. 青海畜牧兽医杂志, 2021, 51(6): 5-10.
	LI S Q, ZHANG X Y, HU G Y, et al. Analysis of alpha-toxin production ability and immunogenicity test of C-type Clostridium perfringens[J]. Chinese Qinghai Journal of Animal and Veterinary Sciences, 2021, 51(6): 5-10. (in Chinese with English abstract)
[27]	刘泽文, 邓均华, 杨克礼, 等. 仔猪大肠埃希氏菌、C型产气荚膜梭菌的灭活及脱毒方法研究[J]. 安徽农业科学, 2010, 38(20): 10702-10703.
	LIU Z W, DENG J H, YANG K L, et al. Inactivation and detoxification of Escherichia coli and C-type Clostridium wechii[J]. Journal of Anhui Agricultural Sciences, 2010, 38(20): 10702-10703. (in Chinese with English abstract)

组别 Group	接种数量 Vaccination quantity	接种剂量 Vaccination dose/mL	结果 Results
二联苗Bivalent vaccine	5	0.5	5/5健活Living
E苗E vaccine	5	0.5	5/5健活Living
C苗C vaccine	5	0.5	5/5健活Living
进口二联苗Imported bivalent vaccine	5	0.5	5/5健活Living

组别 Group	接种数量 Vaccination quantity	接种剂量 Vaccination dose/mL	结果 Results
二联苗Bivalent vaccine	5	0.5	5/5健活Living
E苗E vaccine	5	0.5	5/5健活Living
C苗C vaccine	5	0.5	5/5健活Living
进口二联苗Imported bivalent vaccine	5	0.5	5/5健活Living

组别 Group	接种数量 Vaccination quantity	接种剂量 Vaccination dose/mL	结果 Results
二联苗Bivalent vaccine	5	4	5/5健活Living
E苗E vaccine	5	8	5/5健活Living
C苗C vaccine	5	8	5/5健活Living
进口二联苗Imported bivalent vaccine	5	4	5/5健活Living
对照组Control	5	0	5/5健活Living

组别 Group	接种数量 Vaccination quantity	接种剂量 Vaccination dose/mL	结果 Results
二联苗Bivalent vaccine	5	4	5/5健活Living
E苗E vaccine	5	8	5/5健活Living
C苗C vaccine	5	8	5/5健活Living
进口二联苗Imported bivalent vaccine	5	4	5/5健活Living
对照组Control	5	0	5/5健活Living

组别 Group	疫苗注射不同时间(天)后仔猪体温Body temperature of weaned piglets after different vaccine injection time (days)
组别 Group	1	2	3	4	5	6	7	8	9	10	11	12	13	14
二联苗Bivalent vaccine	39.0	39.0	38.9	39.0	38.9	39.3	39.2	39.1	38.8	39.0	39.1	38.8	39.0	39.1
E苗E vaccine	39.1	39.1	38.9	38.8	39.1	39.2	39.1	39.4	39.1	39.0	39.1	39.0	39.0	39.2
C苗C vaccine	38.9	39.2	39.0	38.7	39.1	39.2	39.4	39.0	39.2	38.8	39.0	39.2	39.2	39.0
进口二联苗 Imported bivalent vaccine	39.1	39.2	39.0	39.0	39.0	39.2	39.0	38.9	39.1	39.1	38.9	39.4	39.1	38.9
对照组Control	39.0	38.9	39.1	39.2	39.1	39.1	38.9	39.2	39.3	39.1	38.9	39.3	39.2	39.1

仔猪红黄痢二联灭活疫苗与同类制品对比研究

Comparative study on inactivated vaccines and similar products against Escherichia coli and Clostridium perfringens

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 27

相关文章 15

编辑推荐

Metrics

本文评价

组别 Group	纤毛类型 Cilia type	攻毒菌株 Challenge strains	攻毒量 Challenge amount/CFU	攻毒数 Challenge number	发病数 Diseased number	发病保护率 Disease protection rate/%
二联苗Bivalent vaccine	K88	C83549	1.5×10¹⁰	5	0	100
	K99	C83644	1.5×10¹⁰	5	1	80
	987P	C83710	1.5×10¹⁰	5	0	100
E苗E vaccine	K88	C83549	1.5×10¹⁰	5	0	100
	K99	C83644	1.5×10¹⁰	5	1	80
	987P	C83710	1.5×10¹⁰	5	1	80
进口二联苗 Imported bivalent vaccine	K88	C83549	1.5×10¹⁰	5	0	100
	K99	C83644	1.5×10¹⁰	5	1	80
	987P	C83710	1.5×10¹⁰	5	1	80
非免疫攻毒对照组 Non immune challenge control group	—	C83549	1.5×10¹⁰	5	5	0
		C83644	1.5×10¹⁰	5	5	0
		C83710	1.5×10¹⁰	5	5	0
正常对照组Normal control	—	—	—	—	5	—

组别 Group	毒素攻击剂量 Toxin challenge dose	攻毒数 Challenge number	死亡数 Death number	死亡保护率 Death protection rate/%
二联苗Bivalent vaccine	1个仔猪最小致死剂量Minimum lethal dose for 1 piglet	5	0	100
E苗E vaccineImported bivalent vaccine	1个仔猪最小致死剂量Minimum lethal dose for 1 piglet	5	0	100
进口二联苗 Imported bivalent vaccine	1个仔猪最小致死剂量Minimum lethal dose for 1 piglet	5	0	100
非免疫攻毒对照组Non immune challenge control group	1个仔猪最小致死剂量Minimum lethal dose for 1 piglet	5	5	0
正常对照组Normal control	—	5	0	—

[1]	王志鹏, 赵剑, 黄盼, 崔雪梅, 南黎, 宋厚辉, 鲍国连, 刘燕. 兔源大肠埃希菌噬菌体分离鉴定与生物学特性研究[J]. 浙江农业学报, 2022, 34(8): 1599-1608.
[2]	汤佳宁, 王永侠, 刘金松, 曾新福, 杨彩梅. 中链脂肪酸及其酯对致病菌的抑菌作用[J]. 浙江农业学报, 2021, 33(9): 1611-1616.
[3]	王伟, 滚双宝, 王鹏飞, 黄晓宇, 谢开会, 雒瑞瑞, 高小莉, 张博, 闫尊强, 杨巧丽, 马艳萍. 猪miR-204组织表达与重要靶基因筛选[J]. 浙江农业学报, 2020, 32(9): 1564-1573.
[4]	段倩倩, 毛天骄, 韩业芹, 韩雪姣, 魏建忠, 孙裴, 李郁. 猪链球菌2型灭活疫苗对小鼠的免疫效果评价[J]. 浙江农业学报, 2020, 32(4): 577-585.
[5]	杨华, 马艳, 刘秀婷, 吕文涛, 卢立志, 肖英平. 即食鸭制品大肠埃希菌污染状况、毒力基因和耐药特征分析[J]. 浙江农业学报, 2020, 32(10): 1841-1848.
[6]	常江, 罗怡, 唐标, 张玲, 戴贤君, 裘罕琦, 杨华, 夏效东. 一株分离自鸡肉样品的多重耐药大肠埃希菌的全基因组测序及耐药性研究[J]. 浙江农业学报, 2019, 31(8): 1249-1256.
[7]	陈章, 刘晓露, 姚焱彬, 吴琼娟, 孙裴, 魏建忠, 李东风, 李郁. 猪丹毒丝菌灭活疫苗免疫佐剂的筛选[J]. 浙江农业学报, 2019, 31(11): 1803-1811.
[8]	彭珂楠, 周雪珂, 殷鑫欢, 李飞, 蔡瑶, 曾喻兵, 江朝源, 张儒博, 杨泽晓, 徐志文, 朱玲. 四川地区猪源致病性大肠埃希菌分子分群、生物被膜形成能力及耐药性研究[J]. 浙江农业学报, 2019, 31(10): 1599-1607.
[9]	王柳杰, 房春林, 古小彬, 谢跃, 杨光友. 紫茎泽兰透皮剂的制备工艺优化及其临床杀螨效果观察[J]. 浙江农业学报, 2018, 30(4): 576-585.
[10]	刘翠艳, 陈四玉, 孙桃桃, 尚莉莉, 杨明川, 杨孜生, 韩春杨. 马齿苋-益生菌联用对热毒证小鼠腹泻的预防效果[J]. 浙江农业学报, 2018, 30(2): 211-219.
[11]	杨华, 何祥祥, 肖英平, 钱鸣蓉, 张巧艳, 唐标. 浙江省三地区冷鲜鸡中大肠埃希菌耐药谱测定及MLST分子分型分析[J]. 浙江农业学报, 2017, 29(6): 888-893.
[12]	黄书伦, 郑凯文, 胡洋, 李章程, 程方俊, 宋振辉, 周作勇, 刘国林, 林春发, 林永润, 张婷婷. 鸭源大肠杆菌Ⅰ型菌毛的鉴定及FimA、FimH基因特征分析[J]. 浙江农业学报, 2017, 29(12): 1994-1999.
[13]	刘勇亮, 陈波, 刘明刚, 徐毓琴, 戴茜茜, 谷笑笑, 潘康成. 连梅止痢中药制剂对大肠埃希菌K88感染仔猪的疗效[J]. 浙江农业学报, 2016, 28(9): 1493-1500.
[14]	耿文学, 唐波, 华涛, 侯继波, 张道华, 许家荣. 免疫增强剂对猪伪狂犬灭活疫苗的免疫增强作用[J]. 浙江农业学报, 2016, 28(11): 1828-1833.
[15]	陆凤1，2，谭磊2，王欣2，包世俊2，任峰2，张凡庆2，刘芳2，仇旭升2，宋翠萍2，孙英杰2，伏小平1，，丁铲2，. 滑液支原体灭活疫苗体外诱导T淋巴细胞增殖及免疫保护研究[J]. 浙江农业学报, 2015, 27(6): 944-.