关键词: 水稻, 聚类分析, 遗传多样性, 微卫星标记

Abstract: In order to explore the diversity of rice varieties, a total of 42 SSR markers were used to identify the diversity of 137 japonica rice varieties including some landraces and cultivars grown in different periods. Of the 42 SSR markers, 33 were polymorphic, and 107 alleles were present in the 33 polymorphic loci.The mean number of alleles per locus (Na) was 3.2, with a range from 2 to 7. The average genetic diversity index of Nei was 0.290, with a range from 0.162 to 0.419. The differentiation degree of indica-japonica showed obvious difference in 137 japonica varieties, and Tdj value ranged from 0.3620-0.9445. Most varieties differentiated thoroughly, and a few japonica landraces and cultivars grown in early period showed indica type. UPGMA cluster analysis showed that all varieties could be differentiated easily, and the result was consistent with classifying based on morphological index. The japonica variety groups could be further classified into several groups. SSR analysis showed the genetic diversity of varieties popularized recently was low, exploring new germplasms and expanding genetic diversity was necessary.

Key words: rice, cluster analysis, genetic diversity, SSR