2013—2016年四川省猪繁殖与呼吸综合征病继发细菌感染的病原学分析

doi:10.3969/j.issn.1004-1524.2017.09.03

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (9): 1437-1444.DOI: 10.3969/j.issn.1004-1524.2017.09.03

2013—2016年四川省猪繁殖与呼吸综合征病继发细菌感染的病原学分析

李幽幽¹, 李小璟¹, 龚双燕¹, 敖英¹, 朱玲^{1, 2, *}, 徐志文^{1, 2}

1.四川农业大学动物医学院,四川成都 611134;
2.四川农业大学四川省动物疫病与人类健康重点实验室,四川成都 611134

收稿日期:2017-04-11 出版日期:2017-09-20 发布日期:2017-09-27
通讯作者: 朱玲,E-mail:abtcxzl72@126.com
作者简介:李幽幽(1992—),女,四川江油人,硕士研究生,从事动物传染病病原分子生物学方向的研究。E-mail:sclyy@hotmail.com
基金资助:
国家“十二五”科技支撑计划(2015BAD12B04-2.3); 四川省科技支撑计划 (2014NZ0043)

Etiology analysis of the bacterial secondary infection of PRRSV in Sichuan Province from 2013 to 2016

LI Youyou¹, LI Xiaojing¹, GONG Shuangyan¹, AO Ying¹, ZHU Ling^{1, 2, *}, XU Zhiwen^{1, 2}

1. College of Veterinary Medical, Sichuan Agricultural University, Chengdu 611134, China;
2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611134, China

Received:2017-04-11 Online:2017-09-20 Published:2017-09-27

摘要/Abstract

摘要： 为给近几年临床控制和治疗PRRSV继发感染提供参考依据,对2013—2016年来自四川省各地感染PRRSV的112头病猪病料进行细菌分离,经形态学观察、选择培养、生化鉴定。分离得到葡萄球菌(MRS)、链球菌、多杀性巴氏杆菌(PM)、大肠埃希菌(Escherichia coli)、猪胸膜肺炎放线杆菌(App)。其中葡萄球菌的分离率最高(70%),其次为多杀性巴氏杆菌(50%),大肠埃希菌(40%),猪胸膜肺炎放线杆菌(40%),链球菌(30%);继发感染主要发生于肝脏和肺脏,其次是脾和心包积液。动物试验结果表明,多杀性巴氏杆菌、大肠埃希菌、链球菌、胸膜肺炎放线杆菌具有致病性。药敏试验结果表明: 多杀性巴氏杆菌对环丙沙星敏感,对庆大霉素中度敏感;大肠埃希菌对丁胺卡那敏感,对氨苄西林、庆大霉素、新霉素中度敏感;链球菌对卡那霉素中度敏感;猪胸膜肺炎放线菌对环丙沙星中度敏感。

关键词: 猪繁殖与呼吸综合征, 细菌, 继发感染, 病原学

Abstract: This experiment was conducted to provide references for clinical management and treatment of PRRSV secondary infections. In this study, morphology observation, selective culture, biochemical identification were used to identify the secondary infective bacteria of PRRSV around 112 piglets from Sichuan province in 2013-2016. Staphylococcus aureus, Streptococcus, Pasteurella multocida, Escherichia coli and Actinobacillus pleuropeumoniae were isolated. Isolation rate of Staphylococcus aureus was the highest (70%), followed by Pasteurella(50%), Escherichia coli (40%), Actinobacillus pleuropneumoniae(40%), Streptococcus (30%). Secondary infection occurred mainly in the liver and lungs, followed by spleen and pericardial effusion. Animal experiments showed that Pasteurella multocida, Escherichia coli, Streptococcus, Actinobacillus pleuropneumoniae were pathogenic. Susceptibility testing showed that Pasteurella multocida was highly sensitive to ciprofloxacin, moderately sensitive to gentamicin; Escherichia coli was highly sensitive to amikacin, and moderately sensitive to ampicillin, gentamicin and neomycin; Streptococcus was moderately sensitive to kanamycin; Actinobacillus pleuropneumoniae was moderately sensitive to ciprofloxacin.

Key words: PRRSV, bacteria, secondary infection, etiology

中图分类号:

S855.1

李幽幽, 李小璟, 龚双燕, 敖英, 朱玲, 徐志文. 2013—2016年四川省猪繁殖与呼吸综合征病继发细菌感染的病原学分析[J]. 浙江农业学报, 2017, 29(9): 1437-1444.

LI Youyou, LI Xiaojing, GONG Shuangyan, AO Ying, ZHU Ling, XU Zhiwen. Etiology analysis of the bacterial secondary infection of PRRSV in Sichuan Province from 2013 to 2016[J]. , 2017, 29(9): 1437-1444.

参考文献

[1] 王金凤. 河北省2007—2011年猪繁殖与呼吸综合征病毒的分子流行病学调查 [D]. 保定:河北农业大学,2011.
WANG J F. Investigation on molecular epidemiology of porcine reproductive and respiratory syndrome virus in Hebei in 2007-2011 [D]. Baoding: Hebei Agricultural University, 2011. (in Chinese with English abstract)
[2] JUSA E R, INABA Y, KOHNO M, et al. Hemagglutination with porcine reproductive and respiratory syndrome virus[J]. Journal of Veterinary Medical Science , 1996, 58(6):521-527.
[3] BENFIELD D A, NELSON E, COLLINS J E, et al. Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332)[J]. Journal of Veterinary Diagnostic Investigation , 1992, 4(2):127-133.
[4] 马德慧,马国文,丁英,等. 猪生殖与呼吸综合征病理损害与免疫抑制观察[J]. 中国兽医科技,2001, 31(6):15-16.
MA D H, MA G W, DING Y, et al. Porcine reproductive and respiratory syndrome pathological damage and immune suppression[J]. Chinese Journal of Veterinary Science and Technology , 2001, 31(6):15-16.(in Chinese)
[5] 戴杰. 猪繁殖与呼吸综合症病毒引发的免疫抑制[J]. 内蒙古畜牧科学,2002 (4):15-18.
DAI J. Porcine reproductive and respiratory syndrome virus causes immunosuppression[J]. Inner Mongolia Animal Husbandry Science , 2002 (4):15-18. (in Chinese)
[6] 刘生杰,刘成文. 猪蓝耳病及继发感染试验诊断与综合防制研究[J]. 中国农学通报. 2007, 23(9):40-43.
LIU S J, LIU C W. Research on diagnose by experiment and integrate control on swine's PRRS and secondary infection[J]. Chinese Agricultural Science Bulletin , 2007, 23(9):40-43. (in Chinese with English abstract)
[7] 曾秀玲,田海蓉,王慧,等. 猪繁殖与呼吸障碍综合征病毒继发细菌感染病例的实验室诊断[J]. 贵州畜牧兽医,2016,40(1):20-22.
ZENG X L, TIAN H R,WANG H, et al. Laboratory diagnosis of secondary bacterial infection of PRRSV[J]. Guizhou Animal Husbandry and Veterinary Medicine , 2016, 40(1):20-22. (in Chinese with English abstract)
[8] 苗得园,张培君,龚玉梅,等. 猪生殖与呼吸综合症病原学及诊断方法研究进展[J]. 北京农业科学,1998,16(1):26-28.
MIAO D Y, ZHANG P J, GONG Y M, et al. Research progress on etiology and diagnosis method of PRRS[J]. Beijing Agricultural Science , 1998, 16(1):26-28. (in Chinese)
[9] 童光志,周艳君,郝晓芳,等. 高致病性猪繁殖与呼吸综合征病毒的分离鉴定及其分子流行病学分析[J]. 中国预防兽医学报,2007,29(5):323-327.
TONG G Z, ZHOU Y J, HAO X F, et al. Identification of a highly pathogenic porcine reproductive and respiratory syndrome virus and molecular epidemiology of the virus[J]. Chinese Journal of Preventive Veterinary Medicine , 2007, 29(5):323-327. (in Chinese with English abstract)
[10] 彭长凌,高崧,刘秀梵. PRRSV人工单独感染及其与PCV2人工联合感染的病理学比较[J]. 徐州医学院学报,2006,26(1):49-52.
PENG C L, GAO S, LIU X F. Comparison on pathological changes between simple PRRSV infection and coinfection of PRRSV and PCV2[J]. Acta Academiae Medicinae Xuzhou , 2006, 26(1):49-52. (in Chinese with English abstract)
[11] 易新健. 副猪嗜血杆菌的分离鉴定与三价灭活疫苗的初步研制 [D]. 扬州:扬州大学,2015.
YI X J. Isolation and identification of Haemophilus parasuis and preliminary development of trivalent inactivated bacterin [D]. Yangzhou: Yangzhou University, 2015. (in Chinese with English abstract)
[12] 宋之先,黄俊明. 免疫接种对猪肺炎支原体加剧PRRSV肺炎的影响[J]. 预防兽医学进展,2000,2(4):1244-1252.
SONG Z X, HUANG J M. Effect of immunization on mycoplasma pneumonia in pigs with PRRSV pneumonia[J]. Preventive Veterinary Medicine Progress , 2000, 2(4):1244-1252. (in Chinese)
[13] 李玉保,马飞,孟喜龙,等. PRRSV对仔猪免疫力的影响[J]. 黑龙江畜牧兽医,2009 (1):67-69.
LI Y B, MA F, MENG X L, et al. Effect of PRRSV on immune function of piglets[J]. Heilongjiang Animal Science and Veterinary Medicine , 2009 (1):67-69. (in Chinese)

[1]	吴俊静, 乔木, 周佳伟, 梅书棋, 彭先文. 猪长链非编码RNA lnc-000649在PRRSV感染增殖中的作用[J]. 浙江农业学报, 2021, 33(2): 223-229.
[2]	江宇航, 李宏伟, 蔡赛波, 林连兵, 张棋麟. 马尾松毛虫肠道细菌的分离鉴定与产蛋白酶细菌的筛选[J]. 浙江农业学报, 2020, 32(8): 1446-1456.
[3]	刘紫英, 袁斌, 肖花美, 吴永飞, 刘小林, 胡祥飞. 马铃薯致病疫霉及其拮抗菌的筛选与鉴定[J]. 浙江农业学报, 2020, 32(5): 840-848.
[4]	张珏锋, 俞叶飞, 李芳, 钟海英, 陈建明. 采用MiSeq测序技术分析3种飞虱中肠内容物的菌群结构[J]. 浙江农业学报, 2020, 32(12): 2192-2200.
[5]	赖家豪, 宋水林, 刘冰. 三株柑橘溃疡病生防内生细菌对脐橙感染溃疡病后几种防御酶活性的影响[J]. 浙江农业学报, 2020, 32(11): 1994-2000.
[6]	沈璐, 李勤超, 田中贵, 稻村达也, 伊日布斯. 太阳热处理对小白菜根肿病防治效果的研究[J]. 浙江农业学报, 2020, 32(1): 98-107.
[7]	刘珊珊, 韦鑫, 盛福瑞, 乔清华. 棉花不同发育时期根际微生物的动态变化[J]. 浙江农业学报, 2019, 31(8): 1361-1371.
[8]	曹艳, 夏其乐, 陈剑兵, 单之初. 以浸米水为原料生产细菌纤维素[J]. 浙江农业学报, 2019, 31(11): 1918-1925.
[9]	徐晓锋, 郭成. 淀粉诱导奶牛乳脂下降后奶牛瘤胃细菌菌群变化[J]. 浙江农业学报, 2019, 31(10): 1591-1598.
[10]	钟永军, 何昕蔚, 余达勇, 蒋晶晶, 江景勇. 台州猕猴桃溃疡病病原菌分子鉴定及药剂筛选[J]. 浙江农业学报, 2018, 30(9): 1548-1554.
[11]	巴少波, 石林, 李群景, 刘正奎, 陈琳, 王晓杜, 宋厚辉. 猪繁殖与呼吸综合征病毒17-ZJ-HZ毒株的分离鉴定与分子流行病学分析[J]. 浙江农业学报, 2018, 30(8): 1303-1311.
[12]	王宗沛, 汪审岳, 姜俊保, 卢磊. 2013—2016年宁海县畜禽重大传染病免疫状况监测分析[J]. 浙江农业学报, 2018, 30(4): 554-559.
[13]	杨颖, 唐伟敏, 陆胜民. 加工条件对细菌纤维素凝胶理化性质的影响[J]. 浙江农业学报, 2018, 30(4): 661-665.
[14]	叶日英, 徐德峰, 孙力军, 王雅玲, 王俊峰, 莫日坚, 王嘉伟, 包艳. 海洋源高产葡萄糖氧化酶细菌的筛选和主要酶学性质[J]. 浙江农业学报, 2018, 30(4): 672-678.
[15]	杨颖, 唐伟敏, 邢建荣, 郑美瑜, 陆胜民. 以橘渣为原料间歇振荡法生产细菌纤维素[J]. 浙江农业学报, 2018, 30(2): 307-313.

2013—2016年四川省猪繁殖与呼吸综合征病继发细菌感染的病原学分析

Etiology analysis of the bacterial secondary infection of PRRSV in Sichuan Province from 2013 to 2016

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价