转cry1Ab/cry2Aj基因玉米双抗12-5对意大利蜜蜂成虫的影响

doi:10.3969/j.issn.1004-1524.2019.11.09

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (11): 1834-1840.DOI: 10.3969/j.issn.1004-1524.2019.11.09

转cry1Ab/cry2Aj基因玉米双抗12-5对意大利蜜蜂成虫的影响

姜媛媛^1,2, 纪艺^1,2, 来勇敏², 祝旋², 陈笑芸², 徐俊锋², 马莲菊^1,*, 徐晓丽^2,*

1.沈阳师范大学生命科学学院,辽宁沈阳 110034;
2.浙江省农业科学院农产品质量标准研究所省部共建农产品质量安全国家重点实验室,浙江杭州 310021

收稿日期:2019-07-25 出版日期:2019-11-25 发布日期:2019-12-04
通讯作者: *,马莲菊,E-mail: malianju@163.com;徐晓丽,E-mail: xuxiaoli@zju.edu.cn
作者简介:姜媛媛(1994—),女,辽宁海城人,硕士研究生,研究方向为生物化学与分子生物学。E-mail: 406599495@qq.com

Potential effect of genetically modified maize DR 12-5 on honey bee (Apis mellifera)

JIANG Yuanyuan^1,2, JI Yi^1,2, LAI Yongmin², ZHU Xuan², CHEN Xiaoyun², XU Junfeng², MA Lianju^1,*, XU Xiaoli^2,*

1. College of Life Science, Shenyang Normal University, Shenyang 110034, China;
2. Institute of Quality and Standard of Agro-Products, Zhejiang Academy of Agricultural Sciences, State Key Laboratory for Quality and Safety of Agro-Products, Hangzhou 310021, China

Received:2019-07-25 Online:2019-11-25 Published:2019-12-04

摘要/Abstract

摘要： 转cry1Ab/cry2Aj基因玉米双抗12-5是我国自主研发的抗虫耐除草剂玉米品系,目前已获批准进入生产性试验阶段。蜜蜂是具有重要生态和经济意义的昆虫,是转基因植物环境安全评价中主要的非靶标节肢动物。在转基因抗虫植物种植过程中,蜜蜂可能通过采集花粉暴露于转基因抗虫植物表达的杀虫蛋白。本实验采用室内生测的方法评价了转基因玉米双抗12-5花粉和Cry1Ab杀虫蛋白对意大利蜜蜂(Apis mellifera)的影响。结果显示,喂食转基因玉米花粉处理组的意大利蜜蜂体内能检测到Cry1Ab杀虫蛋白;与对照相比,饲喂转基因玉米花粉和8 μg·mL^-1纯化的Cry1Ab杀虫蛋白对意大利蜜蜂的存活率没有显著影响,吡虫啉处理组意大利蜜蜂存活率显著低于花粉和蔗糖处理组。以上结果表明,转基因玉米双抗12-5或Cry1Ab杀虫蛋白对意大利蜜蜂的风险较小。

关键词: 意大利蜜蜂, 转基因玉米双抗12-5, Cry1Ab, 花粉

Abstract: Maize DR 12-5 expessing a fused protein Cry1Ab/Cry2Aj is a genetically modified (GM) event that has been approved for preproduction field testing in China. The ecologically and economic important honey bee (Apis mellifera) is a primary non-target arthropod species in environmental risk assessment (ERA) of GM plants. When GM insect resistant plants are planted in field, pollinators may be exposed to the toxin proteins via ingestion of GM pollen. To assess the potential effect of the DR 12-5 to A. mellifera, a laboratory bioassay was conducted using the DR 12-5 pollen and purified Cry1Ab toxin. The results showed that consumption of the DR12-5 pollen or purified Cry1Ab toxin led to detection of low levels of Cry1Ab toxin in the midgut of A. mellifera adults. Neither the DR 12-5 pollen nor 8 μg·mL^-1 purified Cry1Ab toxin showed an adverse effect on the survival rate of A. mellifera adult. In contrast, feeding with 300 mg·L^-1 imidacloprid caused significant toxic effects. These results indicated the minimal risk for potential negative effects on A. mellifera by exposure to the GM maize DR 12-5 or Cry1Ab toxin.

Key words: Apis mellifera, genetically modified (GM) maize DR 12-5, Cry1Ab, pollen

中图分类号:

S593.3

姜媛媛, 纪艺, 来勇敏, 祝旋, 陈笑芸, 徐俊锋, 马莲菊, 徐晓丽. 转cry1Ab/cry2Aj基因玉米双抗12-5对意大利蜜蜂成虫的影响[J]. 浙江农业学报, 2019, 31(11): 1834-1840.

JIANG Yuanyuan, JI Yi, LAI Yongmin, ZHU Xuan, CHEN Xiaoyun, XU Junfeng, MA Lianju, XU Xiaoli. Potential effect of genetically modified maize DR 12-5 on honey bee (Apis mellifera)[J]. , 2019, 31(11): 1834-1840.

参考文献

[1] HAGENBUCHER S, EISENRING M, MEISSLE M, et al.Interaction of transgenic and natural insect resistance mechanisms against Spodoptera littoralis in cotton[J]. Pest Management Science, 2017, 73(8): 1670-1678.
[2] 王江, 武奉慈, 刘新颖, 等. 转基因抗虫耐除草剂复合性状玉米‘双抗12-5’对亚洲玉米螟的抗性及对草甘膦的耐受性研究[J]. 植物保护, 2016, 42(1): 45-50.
WANG J, WU F C, LIU X Y, et al.Evaluation of transgenic maize‘Shuangkang 12-5’ with complex traits of insect-resistance and glyphosate-resistance for the resistance to Ostrinia furnacalis and tolerance to glyphosate[J]. Plant Protection, 2016, 42(1): 45-50.(in Chinese with English abstract)
[3] 陈耕, 何珊, 韩兰芝, 等. 转cry1Ab+cry1C双价抗虫水稻对二化螟的抗性评价[J]. 中国生物防治学报, 2018, 34(1): 71-78.
CHEN G, HE S, HAN L Z, et al.Evaluation of transgenic cry1Ab+cry1C rice lines for its resistance to Chilo suppressalis[J]. Chinese Journal of Biological Control, 2018, 34(1): 71-78.(in Chinese with English abstract)
[4] BRODSCHNEIDER R, CRAILSHEIM K.Nutrition and health in honey bees[J]. Apidologie, 2010, 41(3): 278-294.
[5] HENDRIKSMA H P, HÄRTEL S, STEFFAN-DEWENTER I. Testing pollen of single and stacked insect-resistant bt-maize on in vitro reared honey bee larvae[J]. PLoS One, 2011, 6(12): e28174.
[6] BABENDREIER D, KALBERER N M, ROMEIS J, et al.Influence of Bt-transgenic pollen, Bt-toxin and protease inhibitor (SBTI) ingestion on development of the hypopharyngeal glands in honeybees[J]. Apidologie, 2005, 36(4): 585-594.
[7] DUAN J, MARVIER M, HUESING J, et al.A meta-analysis of effects of Bt crops on honey bees (Hymenoptera: Apidae)[J]. PLoS One, 2008, 3(1): e1415.
[8] HENDRIKSMA H P, KÜTING M, HÄRTEL S, et al. Effect of stacked insecticidal cry proteins from maize pollen on nurse bees (Apis mellifera carnica) and their gut bacteria[J]. PLoS One, 2013, 8(3): e59589.
[9] NIU L, MA Y, MANNAKKARA A, et al.Impact of single and stacked insect-resistant Bt-cotton on the honey bee and silkworm[J]. PLoS One, 2013, 8(9): e72988.
[10] HAN P, NIU C Y, LEI C L, et al.Quantification of toxins in a Cry1Ac + CpTI cotton cultivar and its potential effects on the honey bee Apis mellifera L.[J]. Ecotoxicology, 2010, 19(8): 1452-1459.
[11] NIU L, MA W, LEI C, et al.Herbicide and insect resistant Bt cotton pollen assessment finds no detrimental effects on adult honey bees[J]. Environmental Pollution, 2017, 230:479-485.
[12] DAI P L, ZHOU W, ZHANG J, et al.Field assessment of Bt cry1Ah corn pollen on the survival, development and behavior of Apis mellifera ligustica[J]. Ecotoxicology and Environmental Safety, 2012, 79: 232-237.
[13] WANG Y Y, DAI P L, CHEN X P, et al.Ingestion of Bt rice pollen does not reduce the survival or hypopharyngeal gland development of Apis mellifera adults[J]. Environmental Toxicology and Chemistry, 2017, 36(5): 1243-1248.
[14] 王园园, 李云河, 彭于发. 转Bt基因植物对蜜蜂的安全性研究进展[J]. 中国科学:生命科学, 2016, 46(5): 584-595.
WANG Y Y, LI Y H, PENG Y F.Progress in the assessment of ecological effects of insect-resistant Bt plants on honey bees[J]. Scientia Sinica(Vitae), 2016, 46(5): 584-595.(in Chinese with English abstract)
[15] XIE X W, CUI Z F, WANG Y N, et al.Bacillus thuringiensis maize expressing a fusion gene Cry1Ab/Cry1AcZM does not harm valued pollen feeders[J]. Toxins, 2018, 11(1): 8.
[16] 孙红炜, 徐晓辉, 李凡, 等. 抗虫耐除草剂玉米杂交种瑞丰1号-双抗12-5对3种主要鳞翅目害虫的抗性[J]. 山东农业科学, 2018, 50(5): 109-114.
SUN H W, XU X H, LI F, et al.Resistances of transgenic maize hybrid‘Ruifeng 1-double resistance 12-5’ with insect and herbicide resistance against three major lepidopteran pests[J]. Shandong Agricultural Sciences, 2018, 50(5): 109-114.(in Chinese with English abstract)
[17] 孙红炜, 李凡, 高瑞, 等. 转cry1Ab/cry2Aj和G10evo-epsps基因玉米中Bt蛋白的时空表达及抗性评价[J]. 生物安全学报, 2018, 27(1): 63-68.
SUN H W, LI F, GAO R, et al.Bt protein spatial-temporal expression and evaluation for resistance of transgenic cry1Ab/cry2Aj and G10evo-epsps maize[J]. Journal of Biosafety, 2018, 27(1): 63-68.(in Chinese with English abstract)
[18] MALONE L, PHAM-DELÈGUE M H. Effects of transgene products on honey bees (Apis mellifera) and bumblebees (Bombus sp.)[J]. Apidologie, 2001, 32(4):287-304.
[19] Organisms E P O G M. Scientific opinion on an application from Pioneer Hi-Bred International and Dow AgroSciences LLC (EFSA-GMO-NL-2005-23) for placing on the market of genetically modified maize 59122 for food and feed uses, import, processing and cultivation under Regulation (EC) No 1829/2003[J]. EFSA Journal, 2013, 11(3): 3135.
[20] LIU B, SHU C, XUE K, et al.The oral toxicity of the transgenic Bt+CpTI cotton pollen to honeybees (Apis mellifera)[J]. Ecotoxicology and Environmental Safety, 2009, 72(4): 1163-1169.
[21] DE SCHRIJVER A, DEVOS Y, DE CLERCQ P, et al.Quality of laboratory studies assessing effects of Bt-proteins on non-target organisms: minimal criteria for acceptability[J]. Transgenic Research, 2016, 25(4): 395-411.
[22] RORTAIS A, ARNOLD G, HALM M P, et al.Modes of honeybees exposure to systemic insecticides: estimated amounts of contaminated pollen and nectar consumed by different categories of bees[J]. Apidologie, 2005, 36:71-83.
[23] CRAILSHEIM K, SCHNEIDER L H W, HRASSNIGG N, et al. Pollen consumption and utilization in worker honeybees (Apis mellifera carnica): dependence on individual age and function[J]. Journal of Insect Physiology, 1992, 38(6): 409-419.
[24] DAI P L, JIA H R, GENG L L, et al.Bt toxin Cry1Ie causes No negative effects on survival, pollen consumption, or olfactory learning in worker honey bees (Hymenoptera: Apidae)[J]. Journal of Economic Entomology, 2016, 109(3): 1028-1033.
[25] WANG Y Y, LI Y H, HUANG Z Y, et al.Toxicological, biochemical, and histopathological analyses demonstrating that Cry1C and Cry2A are not toxic to larvae of the honeybee, Apis mellifera[J]. Journal of Agricultural and Food Chemistry, 2015, 63(27): 6126-6132.

转cry1Ab/cry2Aj基因玉米双抗12-5对意大利蜜蜂成虫的影响

Potential effect of genetically modified maize DR 12-5 on honey bee (Apis mellifera)

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