不同月龄新西兰兔肝中差异蛋白质鉴定与验证

doi:10.3969/j.issn.1004-1524.20221467

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (10): 2299-2310.DOI: 10.3969/j.issn.1004-1524.20221467

不同月龄新西兰兔肝中差异蛋白质鉴定与验证

刘方程^a^,^b(), 王峰^a^,^b, 毕冬琳^a^,^b, 杨东亮^a^,^b, 杨晓莉^a^,^b, 柏家林^a^,^b, 李琼毅^a^,^b^,^*()

a.西北民族大学生物医学研究中心,生物工程与技术国家民委重点实验室,甘肃兰州 730030
b.西北民族大学生命科学与工程学院,甘肃兰州 730030

收稿日期:2022-10-25 出版日期:2023-10-25 发布日期:2023-10-31
作者简介:刘方程(1997—),男,甘肃兰州人,硕士研究生,从事病毒天然免疫机制研究。E-mail:956636176@qq.com
通讯作者: ^*李琼毅,E-mail:lqy@xbmu.edu.cn
基金资助:
国家自然科学基金(31702234);国家自然科学基金(31860696);西北民族大学中央高校基本科研业务费项目(31920190003);甘肃省教育厅高等教育教学成果培育项目(2020GSJXCGPY-07);西北民族大学教学改革研究项目(2020YBJG-43)

Identification and validation of differential proteins in liver of New Zealand rabbits at different months of age

LIU Fangcheng^a^,^b(), WANG Feng^a^,^b, BI Donglin^a^,^b, YANG Dongliang^a^,^b, YANG Xiaoli^a^,^b, BAI Jialin^a^,^b, LI Qiongyi^a^,^b^,^*()

a. Biomedical Research Center, Key Laboratory of Biotechnology and Bioengineering of State Ethnic Affairs Commission, Northwest Minzu University, Lanzhou 730030, China
b. Biomedical Research Center, College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China

Received:2022-10-25 Online:2023-10-25 Published:2023-10-31

摘要/Abstract

摘要：

兔出血症是由兔出血症病毒(Rabbit haemorrhagic disease virus, RHDV)引起的一种兔急性、高度致死性传染病,成年兔死亡率超过90%,但2周龄幼兔不发病。基于幼年兔与成年兔对RHDV的易感性差异,采用串联质谱标记(tadem mass tags, TMT)蛋白质组学技术对幼年兔(2周龄)和成年兔(6月龄)肝中的蛋白质进行检测和定量分析,从幼年兔和成年兔肝中定量获得4 353个蛋白质,筛选出821个差异蛋白质;相较于幼年兔,成年兔肝中294个蛋白质显著上调,527个蛋白质显著下调。GO功能富集分析结果显示,生物学过程主要富集到145个小分子代谢蛋白质、111个与氧化还原过程相关的蛋白质和478个参与代谢过程的蛋白质,细胞组分主要富集到528个细胞外成分蛋白质、139个线粒体蛋白质和366个细胞质蛋白质等,分子功能主要富集到342个催化活性蛋白质、93个氧化还原活性蛋白质和50个辅酶结合蛋白质等。821个差异蛋白质在KEGG Pathway数据库中共注释到47条KEGG信号通路,主要涉及代谢途径、糖代谢、氧化磷酸化作用和剪切小体等通路。幼年兔和成年兔肝中差异蛋白质互作网络分析发现,MRPS15的关联度最高,且差异蛋白质胆固醇酰基转移酶1(ACAT1)在互作网络中具有更多相互作用关系。从蛋白质水平探讨幼年兔和成年兔的感染差异机制,筛选并分析幼年兔和成年兔肝中的差异蛋白质,筛选出的差异蛋白质有望成为RHDV体外扩增细胞系构建的突破点,为构建适宜RHDV体外扩增的细胞系提供新的研究思路。

关键词: 新西兰兔, TMT蛋白质组学, 兔出血症病毒(RHDV), 肝, 差异蛋白质

Abstract:

Rabbit hemorrhagic disease is an acute and highly fatal infectious disease caused by Rabbit hemorrhagic disease virus (RHDV). The mortality rate of adult rabbits is more than 90%, but 2-week-old young rabbits are not affected. Based on the differences in the susceptibility of young rabbits and adult rabbits to RHDV, tandem mass tage (TMT) proteomics technology was used to detect and quantitatively analyze the proteins in liver of young rabbits (2 weeks old) and adult rabbits (6 months old). 4 353 proteins were quantitatively obtained from the liver of young rabbits and adult rabbits, and 821 differential proteins were screened. Among them, 294 proteins were significantly up-regulated and 527 proteins were significantly down-regulated in the liver of adult rabbits compared with young rabbits. The results of GO functional enrichment analysis showed that 145 small molecule metabolic proteins, 111 redox-related proteins and 478 proteins involved in metabolic processes were mainly enriched in biological processes; 528 extracellular component proteins, 139 mitochondrial proteins and 366 cytoplasmic proteins were mainly enriched in cellular components; and 342 catalytic active proteins, 93 redox active proteins and 50 coenzyme binding proteins were mainly enriched in molecular functions. A total of 47 KEGG (Kyoto Encyclopedia of Genes and Genomes) signaling pathways were annotated in 821 liver differential proteins in the KEGG pathway database, mainly involving metabolic pathways, carbon metabolism, oxidative phosphorylation and splicing bodies. The analysis of differential protein interaction network in liver of young and adult rabbits showed that MRPS15 had the highest correlation degree, and the differential protein cholesterol acyltransferase 1 (ACAT1) had more interaction in the interaction network. To explore the mechanism of infection difference between young and adult rabbits from the protein level, and to screen and analyze the differential proteins in liver of young and adult rabbits, it is expected to become a breakthrough point in the construction of RHDV in vitro amplification cell lines, and provide new research ideas for the construction of cell lines suitable for RHDV in vitro amplification.

Key words: New Zealand rabbit, tandem mass tage (TMT) proteomics, rabbit hemorrhagic disease virus (RHDV), liver, differential proteins

中图分类号:

S855.3

刘方程, 王峰, 毕冬琳, 杨东亮, 杨晓莉, 柏家林, 李琼毅. 不同月龄新西兰兔肝中差异蛋白质鉴定与验证[J]. 浙江农业学报, 2023, 35(10): 2299-2310.

LIU Fangcheng, WANG Feng, BI Donglin, YANG Dongliang, YANG Xiaoli, BAI Jialin, LI Qiongyi. Identification and validation of differential proteins in liver of New Zealand rabbits at different months of age[J]. Acta Agriculturae Zhejiangensis, 2023, 35(10): 2299-2310.

图/表 6

参考文献 35

[1]	LIU S J, XUE H P, PU B Q, et al. A new viral disease in rabbit[J]. Animal Husbandry and Veterinary Medicine, 1984, 16(6): 253-255.
[2]	LE GALL G, BOILLETOT E, ARNAULD C, et al. Molecular epidemiology of rabbit haemorrhagic disease virus outbreaks in France during 1988 to 1995[J]. Journal of General Virology, 1998, 79(1): 11-16.
[3]	ALDA F, GAITERO T, SUÁREZ M, et al. Evolutionary history and molecular epidemiology of rabbit haemorrhagic disease virus in the Iberian Peninsula and Western Europe[J]. BMC Evolutionary Biology, 2010, 10(1): 1-10.
[4]	ABRANTES J, LOPES A M, DALTON K P, et al. New variant of rabbit hemorrhagic disease virus, Portugal, 2012—2013[J]. Emerging Infectious Diseases, 2013, 19(11): 1900-1902.
[5]	ABRANTES J, LOPES A M, DALTON K P, et al. Detection of RHDVa on the Iberian Peninsula: isolation of an RHDVa strain from a Spanish rabbitry[J]. Archives of Virology, 2014, 159(2): 321-326.
[6]	WESTCOTT D G, FROSSARD J P, EVEREST D, et al. Incursion of RHDV2-like variant in great Britain[J]. Veterinary Record, 2014, 174(13): 333.
[7]	VALÍČEK L, ŠMÍD B, RODÁK L, et al. Electron and immunoelectron microscopy of rabbit haemorrhagic disease virus (RHDV)[J]. Archives of Virology, 1990, 112(3/4): 271-275.
[8]	ZHU J, WANG X X, QI R B, et al. Hemoglobin subunit beta interacts with the capsid, RdRp and VPg proteins, and antagonizes the replication of rabbit hemorrhagic disease virus[J]. Veterinary Microbiology, 2021, 259: 109143.
[9]	MEYERS G, WIRBLICH C, THIEL H J. Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles[J]. Virology, 1991, 184(2): 677-686.
[10]	DROILLARD C, LEMAITRE E, AMELOT M, et al. Rabbit haemorrhagic disease virus Lagovirus europaeus/GI.1d strain: genome sequencing, in vivo virus replication kinetics, and viral dose effect[J]. BMC Veterinary Research, 2021, 17(1): 257.
[11]	MORISSE J P, LE GALL G, BOILLETOT E. Hépatites d’origine virale des léporidés: introduction et hypothèses étiologiques[J]. Revue Scientifique et Technique De L’OIE, 1991, 10(2): 269-310.
[12]	MIKAMI O, PARK J H, KIMURA T, et al. Hepatic lesions in young rabbits experimentally infected with rabbit haemorrhagic disease virus[J]. Research in Veterinary Science, 1999, 66(3): 237-242.
[13]	PRIETO J M, FERNANDEZ F, ALVAREZ V, et al. Immunohistochemical localisation of rabbit haemorrhagic disease virus VP-60 antigen in early infection of young and adult rabbits[J]. Research in Veterinary Science, 2000, 68(2): 181-187.
[14]	MARQUES R M, COSTA-E-SILVA A, ÁGUAS A P, et al. Early inflammatory response of young rabbits attending natural resistance to calicivirus (RHDV) infection[J]. Veterinary Immunology and Immunopathology, 2012, 150(3/4): 181-188.
[15]	MARQUES R M, TEIXEIRA L, ÁGUAS A P, et al. Immunosuppression abrogates resistance of young rabbits to rabbit haemorrhagic disease (RHD)[J]. Veterinary Research, 2014, 45(1): 1-6.
[16]	NEAVE M, HALL R, HUANG N N, et al. Robust innate immunity of young rabbits mediates resistance to rabbit hemorrhagic disease caused by lagovirus europaeus GI.1 but not GI.2[J]. Viruses, 2018, 10(9): 512.
[17]	GREGG D A, HOUSE C, BERNINGER M. Viral haemorrhagic disease of rabbits in Mexico: epidemiology and viral characterization[J]. Revue Scientifique et Technique De L’OIE, 1991, 10(2): 435-451.
[18]	MITRO S, KRAUSS H. Rabbit hemorrhagic disease: a review with special reference to its epizootiology[J]. European Journal of Epidemiology, 1993, 9(1): 70-78.
[19]	SOLEDAD MARÍN M, MARTÍN ALONSO J, PÉREZ ORDOYO GARCÍA L I, et al. Immunogenic properties of rabbit haemorrhagic disease virus structural protein VP60 expressed by a recombinant baculovirus: an efficient vaccine[J]. Virus Research, 1995, 39(2/3): 119-128.
[20]	ABRANTES J, DROILLARD C, LOPES A M, et al. Recombination at the emergence of the pathogenic rabbit haemorrhagic disease virus Lagovirus europaeus/GI.2[J]. Scientific Reports, 2020, 10: 14502.
[21]	MONTI C, ZILOCCHI M, COLUGNAT I, et al. Proteomics turns functional[J]. Journal of Proteomics, 2019, 198: 36-44.
[22]	ROSS P L, HUANG Y N, MARCHESE J N, et al. Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagents[J]. Molecular & Cellular Proteomics: MCP, 2004, 3(12): 1154-1169.
[23]	YE J Z, WU Y Q, LI M R, et al. Keratin 8 mutations were associated with susceptibility to chronic hepatitis B and related progression[J]. SSRN Electronic Journal, 2020, 221(3):464-473.
[24]	LINDER M, POGGE VON STRANDMANN E. The role of extracellular HSP70 in the function of tumor-associated immune cells[J]. Cancers, 2021, 13(18): 4721.
[25]	TROMBETTA E S, MELLMAN I. Cell biology of antigen processing in vitro and in vivo[J]. Annual Review of Immunology, 2005, 23: 975-1028.
[26]	LIU Q T, HUANG X M, ZHAO D M, et al. Identification of heat shock protein A9 as a Tembusu virus binding protein on DF-1 cells[J]. Virus Research, 2017, 227: 110-114.
[27]	PECORARO A, PAGANO M, RUSSO G, et al. Ribosome biogenesis and cancer: overview on ribosomal proteins[J]. International Journal of Molecular Sciences, 2021, 22(11): 5496.
[28]	LI S J, KUANG M, CHEN L Y, et al. The mitochondrial protein ERAL1 suppresses RNA virus infection by facilitating RIG-I-like receptor signaling[J]. Cell Reports, 2021, 34(3): 108631.
[29]	FEROZ S, MUHAMMAD N, RATNAYAKE J, et al. Keratin-based materials for biomedical applications[J]. Bioactive Materials, 2020, 5(3): 496-509.
[30]	李文静, 李健蕊, 黄梦昊, 等. 细胞角蛋白8对丙型肝炎病毒复制的影响[J]. 药学学报, 2016, 51(6): 913-918.
	LI W J, LI J R, HUANG M H, et al. The influence of intracellular keratin 8 on hepatitis C virus replication[J]. Acta Pharmaceutica Sinica, 2016, 51(6): 913-918. (in Chinese with English abstract)
[31]	GOUDARZI A. The recent insights into the function of ACAT1: a possible anti-cancer therapeutic target[J]. Life Sciences, 2019, 232: 116592.
[32]	POKHREL L, KIM Y, NGUYEN T D T, et al. Synthesis and anti-norovirus activity of pyranobenzopyrone compounds[J]. Bioorganic & Medicinal Chemistry Letters, 2012, 22(10): 3480-3484.
[33]	BRADFORD B R, JIN C Y. Stem-loop binding protein and metal carcinogenesis[J]. Seminars in Cancer Biology, 2021, 76: 38-44.
[34]	LI M, TUCKER L D, ASARA J M, et al. Stem-loop binding protein is a multifaceted cellular regulator of HIV-1 replication[J]. Journal of Clinical Investigation, 2016, 126(8): 3117-3129.
[35]	ALBRIGHT E R, MORRISON K, RANGANATHAN P, et al. Human cytomegalovirus lytic infection inhibits replication-dependent histone synthesis and requires stem loop binding protein function[J]. Proceedings of the National Academy of Sciences of the United States of America, 2022, 119(14): e2122174119.

蛋白质ID Protein ID	蛋白质名称 Protein name	表达情况 Expression
Q8MI17	醇脱氢酶Aldehyde dehydrogenase 1 family member A1	上调Up-regulation
G1SS18	角蛋白8Keratin 8	上调Up-regulation
G1SIJ2	胆固醇酰基转移酶1 Acetyl-CoA acetyltransferase 1	下调Down-regulation
G1U758	角蛋白18 Keratin 18	上调Up-regulation
G1TT06	组蛋白结合蛋白1 Selenium binding protein 1	上调Up-regulation
G1SRF7	热休克蛋白9 Heat shock protein family A member 9	下调Down-regulation
G1SKT4	ATP合成酶、H+转运、线粒体F1复合体、α亚单位1	下调Down-regulation
	ATP synthase, H+transporting, mitochondrial F1 complex, alpha subunit 1
A0A5F9CMG2	3-羟基-3-甲基戊二酰-CoA合成酶2 3-Hydroxy-3-methylglutaryl-CoA synthase 2	下调Down-regulation
G1SXX5	羟基酰基-CoA脱氢酶三功能多酶复合体亚基α	下调Down-regulation
	Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha
G1SJS1	组蛋白2簇H2be Histone 2 cluster H2be	下调Down-regulation
A0A5F9C4D8	UDP葡萄糖焦磷酸化酶2 UDP-glucose pyrophosphorylase 2	上调Up-regulation
G1T1U7	亚甲基四氢叶酸脱氢酶	上调Up-regulation
	Methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1
U3KMH9	苹果酸脱氢酶2 Malate dehydrogenase 2	下调Down-regulation
P21195	脯氨酰4-羟化酶亚基beta Prolyl 4-hydroxylase subunit beta	下调Down-regulation
O18750	热休克蛋白90 beta家族成员1 Heat shock protein 90 beta family member 1	下调Down-regulation
G1T2A7	谷胱甘肽S转移酶α1 Glutathione S-transferase alpha I	上调Up-regulation
G1T295	环氧化物水解酶1 Epoxide hydrolase 1	上调Up-regulation
G1U723	前列腺素-E(2) 9还原酶样Prostaglandin-E(2) 9-reductase-like	上调Up-regulation
A0A5F9C5G3	丝氨酸羟甲基转移酶1 Serine hydroxymethyltransferase 1	上调Up-regulation
G1SNJ7	丙氨酸-乙醛酸氨基转移酶2 Alanine-glyoxylate aminotransferase 2	下调Down-regulation

蛋白质ID Protein ID	蛋白质名称 Protein name	表达情况 Expression
Q8MI17	醇脱氢酶Aldehyde dehydrogenase 1 family member A1	上调Up-regulation
G1SS18	角蛋白8Keratin 8	上调Up-regulation
G1SIJ2	胆固醇酰基转移酶1 Acetyl-CoA acetyltransferase 1	下调Down-regulation
G1U758	角蛋白18 Keratin 18	上调Up-regulation
G1TT06	组蛋白结合蛋白1 Selenium binding protein 1	上调Up-regulation
G1SRF7	热休克蛋白9 Heat shock protein family A member 9	下调Down-regulation
G1SKT4	ATP合成酶、H+转运、线粒体F1复合体、α亚单位1	下调Down-regulation
	ATP synthase, H+transporting, mitochondrial F1 complex, alpha subunit 1
A0A5F9CMG2	3-羟基-3-甲基戊二酰-CoA合成酶2 3-Hydroxy-3-methylglutaryl-CoA synthase 2	下调Down-regulation
G1SXX5	羟基酰基-CoA脱氢酶三功能多酶复合体亚基α	下调Down-regulation
	Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha
G1SJS1	组蛋白2簇H2be Histone 2 cluster H2be	下调Down-regulation
A0A5F9C4D8	UDP葡萄糖焦磷酸化酶2 UDP-glucose pyrophosphorylase 2	上调Up-regulation
G1T1U7	亚甲基四氢叶酸脱氢酶	上调Up-regulation
	Methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1
U3KMH9	苹果酸脱氢酶2 Malate dehydrogenase 2	下调Down-regulation
P21195	脯氨酰4-羟化酶亚基beta Prolyl 4-hydroxylase subunit beta	下调Down-regulation
O18750	热休克蛋白90 beta家族成员1 Heat shock protein 90 beta family member 1	下调Down-regulation
G1T2A7	谷胱甘肽S转移酶α1 Glutathione S-transferase alpha I	上调Up-regulation
G1T295	环氧化物水解酶1 Epoxide hydrolase 1	上调Up-regulation
G1U723	前列腺素-E(2) 9还原酶样Prostaglandin-E(2) 9-reductase-like	上调Up-regulation
A0A5F9C5G3	丝氨酸羟甲基转移酶1 Serine hydroxymethyltransferase 1	上调Up-regulation
G1SNJ7	丙氨酸-乙醛酸氨基转移酶2 Alanine-glyoxylate aminotransferase 2	下调Down-regulation

通路名称 Pathway name	ID	差异蛋白数量 Number of differential proteins
代谢途径Metabolic pathways	ocu01100	179
糖代谢Carbon metabolism	ocu01200	30
氧化磷酸化Oxidative phosphorylation	ocu00190	29
帕金森氏症Parkinson disease	ocu05012	29
剪切小体Spliceosome	ocu03040	26
阿兹海默病Alzheimer disease	ocu05010	30
丙酸代谢Propanoate metabolism	ocu00640	12
乙氧基化物和二甲酸酯代谢通路Glyoxylate and dicarboxylate metabolism	ocu00630	11
类固醇生物合成Steroid biosynthesis	ocu00100	8
丁酸盐代谢通路Butanoate metabolism	ocu00650	9

通路名称 Pathway name	ID	差异蛋白数量 Number of differential proteins
代谢途径Metabolic pathways	ocu01100	179
糖代谢Carbon metabolism	ocu01200	30
氧化磷酸化Oxidative phosphorylation	ocu00190	29
帕金森氏症Parkinson disease	ocu05012	29
剪切小体Spliceosome	ocu03040	26
阿兹海默病Alzheimer disease	ocu05010	30
丙酸代谢Propanoate metabolism	ocu00640	12
乙氧基化物和二甲酸酯代谢通路Glyoxylate and dicarboxylate metabolism	ocu00630	11
类固醇生物合成Steroid biosynthesis	ocu00100	8
丁酸盐代谢通路Butanoate metabolism	ocu00650	9

不同月龄新西兰兔肝中差异蛋白质鉴定与验证

Identification and validation of differential proteins in liver of New Zealand rabbits at different months of age

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 35

相关文章 15

编辑推荐

Metrics

本文评价

[1]	刘芳芳, 陈红林, 刘峰, 许晓军, 黄福勇, 楼宝, 钱豪杰. 雌雄红螯螯虾染色体核型比较分析[J]. 浙江农业学报, 2023, 35(9): 2079-2089.
[2]	熊昕宜, 许泽玉, 何念佳, 何俊博, 陈正礼, 黄超, 刘文涛, 罗启慧. 大豆异黄酮干预肥胖大鼠肝氧化应激及炎症反应[J]. 浙江农业学报, 2022, 34(5): 942-948.
[3]	沈卫锋, 郭琦, 刘莉, 牛宝龙, 翁宏飚, 楼宝. 虾肝肠胞虫(Enterocytozoon hepatopenaei)SWP2基因的克隆、表达及其在虾类病害检测中的应用[J]. 浙江农业学报, 2021, 33(6): 993-1000.
[4]	杜金梁, 曹丽萍, 贾睿, 顾郑琰, 何勤, 徐跑, JENEYGalina, 马玉忠, 殷国俊. 甘草总黄酮对高脂条件下罗非鱼肝损伤的保护作用[J]. 浙江农业学报, 2021, 33(10): 1826-1835.
[5]	朱颍琨, 肖劲邦, 钱柏霖, 姜思汛, 尤留超, 张钺, 刘红, 马莉, 曹随忠, 余树民, 沈留红. 泌乳初期奶牛相关脂肪因子及生理生化指标与脂肪肝的相关性[J]. 浙江农业学报, 2019, 31(5): 722-729.
[6]	王承东, 高琪, 李德生, 张和民, 邓林华, 吴虹林, 陈正礼. 一例原发性肝癌大熊猫的病理学观察[J]. 浙江农业学报, 2018, 30(8): 1336-1340.
[7]	韩春杨, 杨明川, 杨孜生, 冯伉梨, 刘翠艳. 黄精多糖的提取及其对CCl₄致大鼠肝损伤的保护作用[J]. 浙江农业学报, 2018, 30(4): 537-547.
[8]	杨宗英, 张一柳, 胡鲲, 杨先乐, 刘力硕, 张凤翔, 蔡红桂. 溴氰菊酯对中华绒螯蟹肝胰腺氧化胁迫效应和组织结构的影响[J]. 浙江农业学报, 2017, 29(8): 1261-1270.
[9]	李杨，高祝，荣茜，杨晓敏，张睿，李英伦*. “复方柴芩颗粒”防治鸭黄曲霉毒素B1慢性中毒的作用机理 [J]. 浙江农业学报, 2015, 27(8): 1337-.
[10]	刘英娟1，杜金梁2，3，贾睿1，2，曹丽萍2，3，王佳豪1，殷国俊1，2，3，*. 枸杞多糖对四氯化碳致建鲤急性肝损伤的保护作用[J]. 浙江农业学报, 2015, 27(1): 37-.
[11]	邓碧华1，王凯民2，卢宇1，张金秋1，吕芳1，何家惠1，侯继波1，*. 一株江苏新型鸭肝炎病毒的鉴定[J]. 浙江农业学报, 2014, 26(6): 1431-.
[12]	钱妤;孙存鑫;刘文斌*;李向飞;王丽娜;朱杰. 饲粮生物素水平对团头鲂幼鱼肠道消化酶活性、胴体组成及肝脏抗氧化能力的影响[J]. , 2014, 26(2): 0-309314.
[13]	周凡;林玲;何丰;*;丁雪燕;薛辉利;徐勇斌. 恩诺沙星注射对中华鳖肝脏Ⅰ相、Ⅱ相酶活性的影响[J]. , 2013, 25(6): 0-1233.
[14]	陈其煌. 高效液相色谱—串联质谱法测定猪肝中可乐定和赛庚啶[J]. , 2013, 25(3): 0-629.
[15]	边文杰;徐燕;李少南*;朱国念. 毒死蜱与鱼肝微粒体P450的相互作用[J]. , 2011, 23(4): 0-781.