›› 2011, Vol. 23 ›› Issue (3): 0-478.

• 作物科学 •    

不同生态区青稞品种变异的SSR鉴定

吴昆仑,陈丽华,迟德钊*   

  1. 青海省农林科学院/青海省青稞遗传育种重点实验室,青海 西宁 810016
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-05-25 发布日期:2011-05-25

Identification of variation of hulless barley in different ecological regions by SSR markers

WU Kun-lun;CHEN Li-hua;CHI De-zhao*   

  1. Qinghai Academy of Agricultural and Forestry Sciences, Qinghai Key Laboratory of Hulless Barley Genetics and Breeding, Xining 810016, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-05-25 Published:2011-05-25

摘要: 利用SSR分子标记技术鉴定不同生态区的青稞品种。以西宁、拉萨、合作、马尔康、香格里拉5个不同生态区的5个品种(‘昆仑12号’、‘藏青25’、‘甘青5号’、‘阿青6号’、‘康青7号’)共25个材料为试验对象,采用SSR引物检测品种变异情况。结果表明,不同品种的变异率不同,且‘藏青25’的变异率最高(0.264),‘甘青5号’的变异率最低(0.040);不同生态区的变异率也不同,且香格里拉的变异率最高(0.144),合作的变异率最小(0.064)。环境因子的改变使得青稞品种发生了一定程度的变异:‘藏青25’对环境因子较为敏感,生态因子的改变易使该品种发生变化。合作作为参试生态区中品种变异率最低的地区,其生态因子对维持品种的性状表现较为有利;香格里拉环境较为复杂,致使青稞的变异率最高。

关键词: 青稞, 生态区, 变异, SSR

Abstract: Variation of hulless barley cultivars in different ecological regions were identified by SSR markers. Twenty-five materials from 5 different ecological regions including Xining, Lhasa, Hezuo, Maerkang, Shangri-La, five cultivars (Kunlun No.12, Zangqing 25, Ganqing No.5, Ahqing No.6, Kangqing No.7) were selected in the experiment. The results showed that different cultivars showed different genetic variation rates in which Zangqing 25 showed the highest variation rate (0.264), while Gangqing No.5 showed the lowest variation rate (0.040). At the same time different ecological regions had different variation rates, in which Shangri-La's variation rate was the highest (0.144), while in Hezuo, the variation rate reached the lowest (0.064). Changes in environment led to different variation behaviors. Zangqing 25 was the most sensitive to environmental changes. In Hezuo, hulless barley had the lowest variation rate because of its good growing conditions, while complex environment led to the highest variation rate in Shangri-La.

Key words: hulless barley, ecological regions, variation, SSR