关键词: 线虫, 木耳, 双孢蘑菇, 菌丝

Abstract:

In order to confirm the harmfulness of the dominant nematodes isolated from edible mushroom growing substance, the nematode quantity amplification on Auricularia auricular and Agaricus bisporus culture medium and its impact on the mushroom hypha growth were investigated in this study. The results showed that 19 genera including three dominant nematode species, Acrobeloides sp., Mesorhabditis sp. and Aphelenchoides composticola were identified and isolated from the button mushroom substance in Cangnan County, Zhejing Province. The three dominant nematode species of each with 150 individuals were inoculated in test tubes which were filled with the hyphae of A. auricular or A. bisporus. The mushroom hyphae grew normally after one week, the number of the nematodes increased less than 4.5 times of the inoculation amount (except of Mesorhabditis sp., its quantity on the A. bisporus hyphae increased 27 times as the amount of the inoculation); after two weeks, the nematodes could lead to mushroom mycelium untimely black brown and withered. After inoculated the three nematode species respectiviely on the hyphae of A. auricular for 2 or 3 weeks, the nematode quantity of all the 3 species increased about 10 times. On A. bisporus hyphae for 2 weeks, the nematode quantity of Acrobeloides sp., Mesorhabditis sp. and A. composticola increased 19.4, 42.3 and 11.1 times, respectively; for three weeks, the quantity of Acrobeloides sp. and Mesorhabditis sp. increased 60 times, while the quantity of A. composticola increased 10 times. Therefore, it was considered that when the density of the nematodes (Acrobeloides sp., Mesorhabditis sp. and A. composticola) was lower than 10 individuals per cm2 substance, the hyphae growth of A. auricular and A. bisporus was sensory normal within 1 week, but the hyphae would be damaged by the nematodes after 2 weeks．

Key words: nematode, edible mushroom, Auricularia auricular, Agaricus bisporus, hyphae