黑木耳菌丝体液体发酵富硒条件优化及其多糖抗氧化活性

doi:10.3969/j.issn.1004-1524.2017.11.18

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (11): 1903-1911.DOI: 10.3969/j.issn.1004-1524.2017.11.18

黑木耳菌丝体液体发酵富硒条件优化及其多糖抗氧化活性

柴新义, 倪瓒鹏, 于士军, 张微微, 殷培峰

滁州学院生物与食品工程学院,安徽滁州239000

收稿日期:2017-05-10 出版日期:2017-11-20 发布日期:2017-12-05
作者简介:柴新义(1978—),男,安徽萧县人,博士,副教授,研究方向为微生物资源与生物技术。E-mail:xinyianhui@163.com
基金资助:
安徽省教育厅自然科学研究重点资助项目(KJ2015A239)

Optimization of Se-rich liquid fermentation conditions and antioxidant activity of polysaccharide from Auricularia auricula mycelia

CHAI Xinyi, NI Zanpeng, YU Shijun, ZHANG Weiwei, YIN Peifeng

School of Biology and Food Engineering, Chuzhou University, Chuzhou 239000, China

Received:2017-05-10 Online:2017-11-20 Published:2017-12-05

摘要/Abstract

摘要： 对黑木耳菌丝体液体发酵富硒条件及菌丝体多糖抗氧化活性进行了研究,旨在寻找新型富硒抗氧化物质并为其工业化生产提供参考资料。以黑木耳菌丝体的富硒量为测定指标,运用单因素和正交试验对黑木耳菌丝体液体发酵富硒条件进行优化,以DPPH(1,1-二苯基-2-苦基肼)自由基清除率为测定指标,采用DPPH还原法对黑木耳液体发酵菌丝体多糖的抗氧化活性进行初步研究。结果表明,黑木耳菌丝体液体发酵的最适富硒条件为:葡萄糖20 g·L^-1,酵母膏2.5 g·L^-1,硒浓度7.5 μg·mL^-1,pH 6.5,温度28 ℃,转速140 r·min^-1,培养时间5 d。该条件下黑木耳液体发酵的菌丝体富硒量可达853.16 μg·g^-1,较优化前提高了66%。黑木耳菌丝体多糖对DPPH自由基的清除率达76.12%。优化后的培养条件较好地提升了黑木耳菌丝体液体发酵的富硒效果。

关键词: 黑木耳, 液体发酵, 富硒量, 多糖, 抗氧化活性

Abstract: The specific objectives of the study were to determine the optimal culture conditions of Se-accumulation and antioxidant activity of polysaccharide from Auricularia auricula mycelia in order to provide some references for liquid fermentation of Auricularia auricula and industrialization of its polysaccharide production. The single factor and orthogonal experiment was used to get the optimal culture conditions of Auricularia auricula by Se-accumulation of mycelia. The free radical scavenging activity of DPPH (1,1-phenyl-2-hydrazide) was used as the index to study the antioxidant activity of polysaccharide from Auricularia auricula mycelia. The results showed that the optimal culture conditions were as follows: glucose 20 g·L^-1, yeast extract 2.5 g·L^-1, selenium concentration 7.5 μg·mL^-1, pH 6.5,temperature 28 ℃, rotational speed 140 r·min^-1, and cultivation 5 d. Se-accumulation under the optimal culture conditions was 853.16 μg·g^-1,which was 66% higher than that of the original conditions. The antioxidant activity of polysaccharide from Auricularia auricula mycelia in scavenging DPPH radical was 76.12%. Se-accumulation of Auricularia auricula was obviously improved with the optimal culture conditions.

Key words: Auricularia auricula, liquid fermentation, selenium accumulation, polysaccharide, antioxidant activity

中图分类号:

S646.6

柴新义, 倪瓒鹏, 于士军, 张微微, 殷培峰. 黑木耳菌丝体液体发酵富硒条件优化及其多糖抗氧化活性[J]. 浙江农业学报, 2017, 29(11): 1903-1911.

CHAI Xinyi, NI Zanpeng, YU Shijun, ZHANG Weiwei, YIN Peifeng. Optimization of Se-rich liquid fermentation conditions and antioxidant activity of polysaccharide from Auricularia auricula mycelia[J]. , 2017, 29(11): 1903-1911.

参考文献

[1] 程瑶, 程鑫, 李昕, 等. 单片黑木耳栽培管理技术[J]. 中国林副特产, 2011,110(1):43-45.
CHENG Y, CHENG X, LI X, et al. Single Auricularia auricula cultivation management[J]. Forest By-Product and Speciality in China , 2011, 110(1):43-45. (in Chinese with English abstract)
[2] 王海宏, 谢忠枕. 硒的生物学功能及其机理研究[J]. 动物营养学报, 2003, 15 (3):6-11.
WANG H H, XIE Z Z. Research on the biological function mechanism of selenium[J]. Acta Zoonutrimenta Sinica , 2003, 15 (3):6-11. (in Chinese with English abstract)
[3] JR F J, HAMILTON M T, GLENN T C, et al. Dietary selenomethionine administration in the american alligator ( Alligator mississippiensis ): Hepatic and renal Se accumulation and its effects on growth and body condition[J]. Archives of Environmental Contamination & Toxicology , 2017, 72(3):439-448.
[4] SHARMA S, KAUR N, KAUR S, et al. Selenium as a nutrient in biostimulation and biofortification of cereals[J]. Indian Journal of Plant Physiology , 2016,22(1):1-15.
[5] OLAG M T, EKATERINA, ALEXEI N P, et al. Biodegradation of an organoselenium compound to elemental Selenium by Lentinula edodes (Shiitake) mushroom[J]. Biological trace element research , 2012, 149(1):97-101.
[6] YONG J, GUAN X Y, DAI C C. The effects of selenium on polyunsaturated fatty acids of Diasporangium jonesianum [J]. Applied Biochemistry Biotechnology , 2014, 172(2):561-569.
[7] 王献友, 陈培云, 吴广臣,等. 黑木耳多糖提取及其药理活性的研究进展[J]. 南方农业学报, 2012, 43(5):683-687.
WANG X Y, CHEN P Y, WU G C, et al. Advances on polysaccharide extraction from Auricularia auricula and its pharmacological activity[J]. Journal of Southern Agriculture , 2012, 43(5):683-687. (in Chinese with English abstract)
[8] 刘建荣, 温秀荣, 顾雅君. 黑木耳富硒发酵条件的优化[J]. 河北大学学报(自然科学版), 2015, 35(6):616-622.
LIU J R, WEN X R, GU Y J. Optimization of Se-enriched fermentation conditions of Auricularia auricula [J]. Journal of Hebei University ( Natural Science Edition ), 2015, 35(6):616-622. (in Chinese with English abstract)
[9] SHANG D, LI Y, WANG C, et al. A novel polysaccharide from Se-enriched Ganoderma lucidum induces apoptosis of human breast cancer cells[J]. Oncology Reports , 2011, 25(1):267-272.
[10] 黄春燕, 张柏松, 万鲁长, 等. 食用菌富硒培养研究进展[J]. 山东农业科学, 2012, 44(7):81-87.
HUANG C Y, ZHANG B S, WAN L C, et al. Research progress of selenium enriched culture of edible fungi[J]. Shandong Agricultural Sciences , 2012, 44(7):81-87. (in Chinese)
[11] 朱黎霞, 周竹音, 潘丽媛. 黑木耳硒多糖抗肿瘤作用的研究[J]. 中国中医药科技, 2011,18(5):454.
ZHU L X, ZHOU Z Y, PAN L Y. Study on antitumor effect of selenium polysaccharide from Auricularia auricula [J]. Chinese Medicine Science and Technology , 2011, 18(5):454. (in Chinese)
[12] RECZYNSKI W, MUSZYNSK B, OPOKA W, et al. Comparative study of metals accumulation in cultured in vitro mycelium and naturally grown fruiting bodies of Boletus badius and Cantharellus cibarius [J]. Biological Trace Element Research , 2013, 153(1/3):355-362.
[13] SENKA S V, IBRAHIM O M, ZORAN P Z, et al. Antioxidant properties of selected Boletus mushrooms[J]. Food Biophysics , 2010, 5(1):49-58.
[14] 王俊, 黄明, 徐幸莲, 等. 硒及富硒功能食品研究进展[J]. 江苏农业科学, 2003(2): 53-56.
WANG J, HUANG M, XU X L, et al. Advance in selenium and selenium-enriched functional foods[J]. Jiangsu Agricultural Sciences , 2003(2): 53-56. (in Chinese with English abstract)
[15] 柴新义, 王四宝, 樊美珍. 杨树菇深层液体发酵培养基及培养条件的优化[J]. 西北农林科技大学学报(自然科学版), 2006, 34(11):117-120.
CHAI X Y, WANG S B, FAN M Z. Studies on the optimal cultivation of Agrocybe aegerita ’s liquid fermentation[J]. Journal of Northwest A & F University ( Natural Science Edition ), 2006, 34(11):117-120. (in Chinese with English abstract)
[16] 辛树权, 王晓光, 赵骥民, 等. 硒元素对黑木耳深层发酵的影响[J]. 食品科学, 2009, 30(7): 202-208.
XIN S Q, WANG X G, ZHAO J M, et al. Effects of selenium on submerged fermentation of Auricularia auricula [J]. Food Science , 2009, 30(7): 202-208. (in Chinese with English abstract)
[17] 江洁,张文静,季旭颖. 美味牛肝菌富硒培养条件优化及其多糖抗氧化性研究[J]. 中国食品学报,2015,15(12):91-98.
JIANG J, ZHANG W J, JI X Y. Optimization of Se-rich culture conditions and antioxidant activities of polysaccharides from Boletus edulis mycelium[J]. Journal of Chinese Institute of Food Science and Technology , 2015, 15(12):91-98. (in Chinese with English abstract)
[18] TINA S, CHI T H. Antioxidant activities of buckwheat extracts[J]. Food Chemistry , 2005, 90(4): 743-749.
[19] 于克学, 贾乐, 王汉忠. 冬虫夏草深层发酵富硒初步研究[J]. 食品研究与开发, 2003, 24(4): 3-5.
YU K X, JIA L, WANG H Z. Study on Se-accumulation in Cordyceps sinensis by submerged fermentation[J]. Food Research and Development , 2003, 24(4): 3-5. (in Chinese with English abstract)
[20] 刘碧蓉, 温海祥, 萧洪东. 富硒平菇菌种的筛选[J]. 中国食用菌, 2007, 26(3): 14-16.
LIU B R, WEN H X, XIAO H D. Screening on selenium rich strains of Pleurotus ostreatus [J]. Edible Fungi of China , 2007, 26(3): 14-16. (in Chinese with English abstract)
[21] 赵镭, 胡小松. 富硒灵芝的研究进展[J]. 中国食用菌, 2002, 22(3): 35-36.
ZHAO L, HU X S. Researches on the Se-accumulating Ganoderma lucidum [J]. Edible Fungi of China , 2002, 22(3): 35-36. (in Chinese with English abstract)
[22] 江洁, 麦海美, 解彬, 等. 羊肚菌菌丝体富硒条件优化及其硒多糖抗氧化活性研究[J]. 食品与发酵工业, 2016, 42(9): 120-125.
JIANG J, MAI H M, XIE B, et al. Optimization of Se-rich culture conditions and antioxidant activities of polysaccharides from Morchella esculenta mycelium[J]. Food and Fermentation industries , 2016, 42(9): 120-125. (in Chinese with English abstract)
[23] 张恒银, 孙艳丽, 徐良, 等. 黑木耳液体深层发酵培养基的初步筛选[J]. 食用菌, 2006,28(5): 13-14.
ZHANG H Y, SUN Y L, XU L, et al. Preliminary screening of submerged fermentation medium of Auricularia auricula [J]. Edible Fungi , 2006, 28(5): 13-14. (in Chinese)
[24] 王谦, 贾震. 不同条件对黑木耳液体深层发酵的影响[J]. 河北大学学报(自然科学版), 2011, 31(1): 74-78.
WANG Q, JIA Z. Effects of different conditions on the black fungus in submerged fermentation[J]. Journal of Heibei University ( Natural Science Edition ), 2011, 31(1): 74-78. (in Chinese with English abstract)
[25] 肖彩霞, 王玉红, 章克昌. 黑木耳深层发酵工艺条件的研究[J]. 生物技术, 2004, 15(5): 71-72.
XIAO C X, WANG Y H, ZHANG K C. Studies on submerged fermentation of Auricularia auricula [J]. Biotechnology , 2004, 15(5): 71-72. (in Chinese with English abstract)
[26] 柴新义, 于士军, 张微微, 等. 食药用大型真菌抗氧化活性菌株的筛选及其培养条件优化[J]. 食品与机械, 2016, 32(1):125-129.
CHAI X Y, YU S J, ZHANG W W, et al. Screening the strain with antioxidation activity from wild macrofungi and optimization of its fermentation conditions[J]. Food and Machinery , 2016, 32(1):125-129. (in Chinese with English abstract)
[27] 尚德静, 王关林. 四种食用菌富硒能力的比较研究[J]. 食用菌学报, 1999, 6(3): 17-20.
SHANG D J, WANG G L. Comparison of selenium accumulation ability in the mycelia of four edible fungi[J]. Acta Edulis Fungi , 1999, 6(3): 17-20. (in Chinese with English abstract)
[28] 胡文军, 傅庭治, 曹幼琴. 硒在金针菇菌体内的生物转化[J]. 中国食用菌, 1997, 16(3): 30-33.
HU W J, FU T Z, CAO Y Q. Biotransformation of selenium in mycelium of Flammulina velutipes NJ 9601[J]. Edible Fungi of China , 1997, 16(3): 30-33. (in Chinese with English abstract)

黑木耳菌丝体液体发酵富硒条件优化及其多糖抗氧化活性

Optimization of Se-rich liquid fermentation conditions and antioxidant activity of polysaccharide from Auricularia auricula mycelia

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	李园成, 孙宏, 吴逸飞, 姚晓红, 沈琦, 汤江武. 固态发酵棉籽仁的营养成分及其抗氧化活性[J]. 浙江农业学报, 2020, 32(4): 610-615.
[2]	吴卫成, 戴建波, 曹艳, 夏其乐, 陈剑兵, 孟祥河. 物理改性对甘薯皮膳食纤维含量、多糖组成及其结构的影响[J]. 浙江农业学报, 2020, 32(3): 490-498.
[3]	王明哲, 杨颖, 唐伟敏, 刘哲, 孙培龙, 陆胜民. 戊糖乳杆菌YY112产胞外多糖的发酵工艺条件优化[J]. 浙江农业学报, 2020, 32(2): 327-336.
[4]	杨志, 李文义, 高云涛, 熊华斌, 陈毅坚, 杨慧娟. 响应面法优化针叶樱桃总黄酮的提取工艺及其抗氧化活性研究[J]. 浙江农业学报, 2020, 32(10): 1866-1872.
[5]	张文平, 王清, 黄诗宸, 吴佩佳, 程新. 乳酸菌胞外多糖对水稻生长及土壤理化性质的影响[J]. 浙江农业学报, 2019, 31(1): 130-138.
[6]	韩春杨, 杨明川, 杨孜生, 冯伉梨, 刘翠艳. 黄精多糖的提取及其对CCl₄致大鼠肝损伤的保护作用[J]. 浙江农业学报, 2018, 30(4): 537-547.
[7]	陈利华, 王欣, 成家杨. 不同方法提取海洋硅藻粗多糖的比较[J]. 浙江农业学报, 2018, 30(2): 280-289.
[8]	王储炎, 程俊文, 祝超. 黑芝菌发酵产生胞外多糖的工艺优化及其抗氧化活性[J]. 浙江农业学报, 2018, 30(11): 1938-1945.
[9]	高帆, 夏惠, 王秀, 王进, 周琼, 吕秀兰, 梁东. 不同甜樱桃品种抗氧化物质及抗氧化活性分析[J]. 浙江农业学报, 2017, 29(6): 926-932.
[10]	黄芳芳, 尹洁, 严志鹏, 肖卫强, 毕一鸣, 杨艳芹, 周国俊, 牛芳芳, 杨君. 铜藻多糖在烟丝中的保润性能、热裂解及其生物安全性分析[J]. 浙江农业学报, 2017, 29(5): 831-839.
[11]	唐宏刚，肖朝耿，叶梦迪，朱培培，陈黎洪*. 猪骨蛋白酶解液美拉德反应产物的抗氧化活性研究[J]. 浙江农业学报, 2016, 28(8): 1396-.
[12]	张强1，2，吴彩娥1，*. 羊肚菌菌丝体蛋白的理化特性及抗氧化活性[J]. 浙江农业学报, 2016, 28(8): 1408-.
[13]	金群力，张作法，范丽军，蔡为明*. 棕色双孢蘑菇子实体不同生长阶段抗氧化活性比较[J]. 浙江农业学报, 2016, 28(5): 797-.
[14]	李巧自1，高素萍2，*，张科燕1，陈锋1，段晶晶1，蔡心怡1. 外源一氧化氮对铁皮石斛原球茎生长和多糖积累的影响[J]. 浙江农业学报, 2016, 28(4): 595-.
[15]	郑雪良1，刘春荣1，*，王登亮1，毕旭灿2，杨波2，孙崇德3. 胡柚小青果的黄酮类化合物及抗氧化活性研究[J]. 浙江农业学报, 2015, 27(7): 1185-.