›› 2013, Vol. 25 ›› Issue (1): 0-42.

• 论文 •    

全自动扫描和微卫星标记技术分析地方灰鹅遗传多样性

段修军1,徐琪2,乔娜2,陈国宏2,董飚1,孙国波1   

  1. 1国家级水禽基因库,江苏 泰州 225300;2扬州大学 动物科学与技术学院,江苏 扬州 225009
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-01-25 发布日期:2013-01-25

Genetic diversity of indigenous grey goose breeds based on microsatellite markers by automatic scanning technique

DUAN Xiujun;XYU Qi;QIAO Na;CHEN Guohong;DONG Biao;SUN Guobo   

  1. 1National Gene Bank of Waterfowl Germplasm, Taizhou 225300, China; 2College of Animal Science and Technology, Yangzhou University, Yangzhou 225009,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-01-25 Published:2013-01-25

摘要: 运用10对荧光标记微卫星引物分析了狮头鹅、乌鬃鹅、马岗鹅遗传多态性,通过比较不同品种间等位基因的频率、群体遗传参数、遗传距离,为品种起源、保护和开发利用提供参考资料。结果显示:3个鹅品种在10个微卫星座位上共拥有63个等位基因,等位基因频率为0~0.8833。部分等位基因为品种所特有,可以用来识别品种。马岗鹅杂合度最高为0.6388,乌鬃鹅最低为0.5712。3个鹅品种均为高度多态,说明了3个鹅品种都具有丰富的遗传多样性。乌鬃鹅和马岗鹅的遗传距离最近,符合品种形成之间的关系。

关键词: 地方灰鹅, 微卫星标记, 全自动扫描技术, 遗传多样性

Abstract: The genetic polymorphisms of 3 goose breeds(Shitou goose, Wuzong goose and Magang goose)were analyzed by 10 fluorescent labeling microsatellite primers. The allelic frequency, genetic parameter and genetic distance were compared among different breeds. The data would be used to study genesis, conservancy and exploitation about breeds. Sixty\|three allelic were indicated in 10 microsatellites about three goose breeds. The frequency of allelic was between 0 and 08833. Some allelic belong to single breed which could be used to identify different breeds. The heterozygosity of Magang goose (0.6530) was the highest, and Wuzong goose (0.5712) was the lowest. The three goose breeds PIC were all at high level. There was a large of genetic diversity in three goose breeds. The distance of Wuzong goose and Magang goose was the nearest that was accord with genesis of breeds.

Key words: indigenous grey goose, micresatellite marker, automatic analytical method, genetic diversity