›› 2009, Vol. 21 ›› Issue (3): 0-240.

• 论文 •    

温度对克氏原螯虾消化酶活性的影响

祝尧荣,寿建昕,沈文英*   

  1. 绍兴文理学院生命科学学院,浙江绍兴312000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-25 发布日期:2009-05-25

Effects of temperature on activities of digestive enzymes in Cambarus clarkii

ZHU Yao-rong;SHOU Jian-xin;SHEN Wen—ying*   

  1. College of Science of Shaoxing University,Shaoxing,Zhejiang 312000,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-25 Published:2009-05-25

摘要: 分析了一定pH值下不同反应温度对克氏原螯虾3种主要消化酶活性的影响。结果表明:克氏原螯
虾淀粉酶在肝胰脏、肠道、胃的最适温度均为40℃ ;胃蛋白酶在肝胰脏、肠道、胃的最适温度都为35℃;脂肪酶在肝胰脏、肠道、胃的最适温度分别为25,35,25℃ ;胰蛋白酶在肝胰脏、肠道、胃的最适温度分别为55,65,55℃。在最适温度条件下,淀粉酶活性顺序为:胃>肝胰脏>肠;胰蛋白酶和胃蛋白酶活性顺序均为:肝胰脏>胃>肠道;脂肪酶在肝胰脏、肠道活性接近,大于胃内活性。

关键词: 克氏原螯虾, 消化酶活性, 温度

Abstract: Effects of different temperatures on activities of three digestive enzymes in Cambarus clarkii were examined.The results showed that the optimum temperatures for amylase in hepatopancreas,intestine and stomach were all 40℃ ;while the optimum temperatures for pepsin were all 35℃.In addition,the optimum temperatures for lipase in hepatopancreas,intestine and stomach were 25,35,25℃ ,respectively,and the optimum temperatures for trypase were 55,65,55℃ ,respeclively.At the oplimum reactive temperature,the activity order of amylase in different organs was stomach > hepatopancreas
> intestine,meanwhile ,the activity orders of trypase and pepsin were hepatopancreas> stomach > intestine.The activity of lipase in intestine was similar to in hepatopancreas,which was higher than in stomach.

Key words: Cambarus clarkii, temperature, activity of digestive enzymes