›› 2009, Vol. 21 ›› Issue (1): 0-10.

• 论文 •    

苏云金杆菌菌株YJ一2000生长和伴孢晶体形成的适宜培养条件

卢新民 1,陈若霞 2,*,叶恭银 1,*,姚红燕 2,胡 萃 1   

  1. 1 浙江大学昆虫科学研究所,浙江杭州310029;2 宁波市农业科学研究院,浙江宁波315040
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-01-25 发布日期:2009-01-25

Suitable culture conditions for the growth and parasporal crystal production of Bacilus thuringiensis strain YJ-2000

LU Xin-min;CHEN Ruo-xia;*;YE Gong-yin;*;YAO Hong-yan;HU Cui   

  1. 1 Institute of Insect Sciences,Zhejiang University,Hangzhou 310029,China; 2 Ningbo Academy of Agriculture,Ningbo 315040,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-01-25 Published:2009-01-25

摘要: 苏云金杆菌菌株YJ-2000是本所选育的一个对家蚕蚁蚕无毒性,而对多种其他鳞翅目害虫表现强毒性的新菌株。本文通过研究培养基初始pH、碳源、氮源和金属离子对苏云金杆菌菌株YJ一20OO生物量和伴孢晶体产量的影响,以确定该菌生长和伴孢晶体形成的适宜培养条件。结果表明:既满足该菌生长,又能获得最高伴孢晶体产量的该菌的适宜培养条件是,培养基初始pH为6.5,碳源为麦芽糖,氮源为蛋白胨与(NH4)2S04的混合物(比例为1:1),培养基中Mg2+ 和Cu2+ 的浓度各为1×10-3和0.008×10-3mol/L。

关键词: 苏云金杆菌, 生物量, 伴孢晶体, 营养因子, pH, 金属离子

Abstract: The effects of the initial pH of medium,nutrition elements including carbon and nitrogen Soul~es,the concentration of metal ions including Mg2+ and Cu2+ on the growth and parasporal crystal production ofBacillus thurlngiensis strain
YJ-2OO0,which shows no toxicity to the neonate lalwae of the domestic silkworm. Bombyx mori but showed high toxicity to various important lepidopteran species.were studied to define the suitable culture conditions for this strain.The culture conditions,which are the most optimum not only for the high biomass,but also for the highest yield of the parasperal crystals of this strain,were as follow:the initial pH of the medium was 6.5,the medium carbon source was maltose,the medium nitrogen source was peptone plus (NH4)2SO4 with the ratio of 1:1,the concentrations of Mg2+ and Cu2+ in the medium were 1×10-3 mol/L and 0.008×10-3 mol/L, respectively.

Key words: Bacillus thuringiensis, biomass, parasporal crystals, nutrition eleme nts, pH, metal ion