›› 2009, Vol. 21 ›› Issue (05): 0-428.

• 论文 •    

几个番茄品种AFLP指纹图谱的建立

姚金晓,杨悦俭,叶青静,王荣青,周国治,阮美颖   

  1. 浙江省农业科学院蔬菜研究所, 浙江杭州310021
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-09-25 发布日期:2009-09-25

Establishment of the AFLP fingerprinting for some tomato cultivars

YAO Jin-xiao;YANG Yue-jian;YE Qing-jing;WANG Rong-qing;ZHOU Guo-zhi;RUAN Mei-ying   

  1. Institute of Vegetables ,Zhejiang Academy of Agricultural sciences ,Hangzhou 310021, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-09-25 Published:2009-09-25

摘要: 应用优化的AFLP技术体系,构建了7份番茄黄化曲叶病毒病抗病材料和1份感病材料的指纹图谱。根据所得到的DNA指纹图谱数据,进行亲缘关系和遗传多样性分析。从64对引物中筛选出扩增效果稳定、多态性好的5对引物组合,分别对8份材料的基因组DNA进行扩增,共扩增出清晰的条带149条.其中62条带具有多态性,多态性位点百分率为41.6%。这一结果表明供试抗感黄化曲叶病毒病番茄材料在DNA水平上酶切位点的分布存在一定的差异。供试材料间的遗传相似系数的变化范围为0.83—0.95。以遗传相似系数0.89为阈值,进行UPGMA聚类分析,将8份番茄材料分成1个复合组和3个独立组。以上结果在DNA水平上为番茄抗黄化曲叶病毒病育种的亲本选配提供了参考依据。

关键词: 番茄, AFLP, 指纹图谱

Abstract: The genetic relationship and diversity of 8 tomato germplasm resources which were resistant or suscepti- ble to tomato yellow leaf curl disease (TYLCD) were analyzed by the optimized AFLP (amplified fragments length polymorphism). Five primer combinations selected from 64 primer pairs revealed a total number of 149 unambiguous bands,62 of which were polymorphic with a polymorphism frequency of 41.6%. This result showed molecular variations of some enzyme digestion sites among the used tomato germplasm resources. The genetic similarity coefficient of 8 tomato resources ranged from 0.83 to 0.95. Based on the coefficient value of 0.89,these tomato germplasm resources were clustered into 1 multiple-member group and 3 one-member groups by UPGMA (unweighted pair group method with arithmetic mean) analysis ,which provided molecular reference for parent selection in tomato breeding for resistance to TYLCD.

Key words: tomato, AFLP, fingerprinting