›› 2013, Vol. 25 ›› Issue (5): 0-1001.

• 论文 •    

杨梅花粉活力检测及储藏方法研究

戚行江1,任海英1,郑锡良1,邱立军2,梁森苗1,张真真2   

  1. 1浙江省农业科学院 园艺研究所,浙江 杭州 310021;2浙江省舟山市定海区农林局,浙江 舟山 316000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-09-25 发布日期:2013-09-25

Vitality test and storage method for bayberry pollen

QI Xing-jiang;REN Hai-ying;ZHENG Xi-liang;QIU Li-jun;LIANG Sen-miao;ZHANG Zhen-zhen   

  1. 1 Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;2 Dinghai District Bureau of Agriculture and Forestry, Zhoushan 316000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2013-09-25 Published:2013-09-25

摘要: 对杨梅(Myrica rubra Sieb. & Zucc.)花粉活力检测和储藏方法进行了研究。筛选出最佳的杨梅花粉活力离体萌发检测法:用毛笔轻刷花粉至固体培养基(15%蔗糖,0.01%硼酸,1%琼脂)上,22℃黑暗培养10 h,显微观测花粉萌发率。20℃储藏时,经硅胶干燥处理能延长杨梅花粉活力保存时间。与20℃相比,低温保存能明显延长杨梅花粉活力,在4,-20和-80℃保存100 d后,花粉萌发率分别是12%,37.5%和48%。

关键词: 杨梅, 花粉, 活力检测, 贮藏方法

Abstract: In order to find the best storage method for pollen of bayberry (Myrica rubra Sieb. & Zucc.), the study on the optimal examination method for vitality of bayberry pollen was carried out. The results showed that bayberry pollen germinated best on the solid medium containing 15% sucrose, 0.01% boric acid and 1% agar in darkness at 22℃ for 10 h. The silica gel drying could extend the living time of bayberry pollen under 20℃. Compared with 20℃, lower temperature could significantly extend the living time of bayberry pollen. After storage for 100 days at 4℃, -20℃ and -80℃, the pollen germination rate was 12%, 37.5% and 48%, respectively.

Key words: bayberry, pollen, vitality test, storage method