›› 2014, Vol. 26 ›› Issue (1): 0-126.

• 植物保护 •    下一篇

六种杀菌剂对柑橘炭疽病菌的室内毒力和田间防治效果

蒲占湑1,黄振东2,胡秀荣2,李红叶1,   

  1. 1浙江大学 生物技术研究所,浙江 杭州310029;2浙江省柑橘研究所,浙江 黄岩 318020
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2014-01-25 发布日期:2014-07-09

In vitro toxicity and field control effect of six fungicides to Colletotrichum gloeosporioides causing citrus anthracnose

PU Zhan\|xyu;HUANG Zhen\|dong;HU Xiu\|rong;LI Hong\|ye;*   

  1. 1 Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China; 2 Citrus Research Institute of Zhejiang Province, Huangyan 318020, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-01-25 Published:2014-07-09

摘要: 为了筛选高效、低毒、无公害的防治柑橘炭疽病(Colletotrichum gloeosporioides Penz.)的杀菌剂,采用菌丝生长速率法和孢子萌发法分别测定了吡唑醚菌酯、嘧菌酯、咪鲜胺、唑醚·代森联、甲基硫菌灵和丙森锌对柑橘炭疽病菌的室内毒力。室内测定结果表明:6种杀菌剂对柑橘炭疽病菌菌丝抑制的有效中浓度(EC50)分别为081,258,0066,497,1 55515和23798 μg·mL-1,吡唑醚菌酯、嘧菌酯、咪鲜胺和唑醚·代森联对该病孢子萌发EC50分别为093,1434,447和529 μg·mL-1。田间试验结果表明:嘧菌酯、咪鲜胺、丙森锌和唑醚·代森联在试验剂量下对炭疽病的防效在95%以上。综合室内外试验结果得知,嘧菌酯、咪鲜胺、丙森锌和唑醚·代森联是防治柑橘炭疽病的有效药剂。

关键词: 柑橘炭疽病, 杀菌剂, 毒力测定, 田间防治效果

Abstract: In order to obtain fungicides with high efficiency, low toxicity, and less pollution in controlling citrus anthracnose, which caused by Colletotrichum gloeosporioides Penz., the toxicity of pyraclostrobin, azoxystrobin, prochloraz, pyraclostrobin·me\|tiram, thiophanate\|methyl and iprovalicarb to a strain of C. gloeosporioides was respectively tested by mycelium growth rate and spore germination. The results showed that the effective dose to reduce mycelial growth of C. gloeosporioides by 50% (EC50 values) were 081, 258, 0066, 497, 155515 and 23798 μg·mL-1, whereas the EC50 values of spore germination to pyraclostrobin, azoxystrobin, prochloraz and pyraclostrobin·me\|tiram were 093, 1434, 447 and 529 μg·mL-1. Field experiments showed that 95% of above citrus anthracnose could be inhibited by azoxystrobin, prochloraz, iprovalicarb and pyraclostrobin·me\|tiram with the recommended concentrations on citrus anthracnose. Conclusively, azoxystrobin, prochloraz, iprovalicarb and pyraclostrobin·me\|tiram are effective in controlling citrus anthracnose.

Key words: citrus anthracnose, fungicide, toxicity test, field controlling effect