关键词: 葡萄, 茎尖, 脱毒, 快繁

Abstract: Zhuangyuanhong grape was used as experimental material， the shoot tips of stem apexes were selected as explants， which reproduced rapidly through directly inducing cluster buds， then the methods of heat treatment and tip culture were combined to induce virus\|free grape. The results showed that shoots induction rate in medium 1/2MS was higher than MS. The best inducing effects was found in the medium of 1/2 MS+20 mg·L-1 6\|BA+01 mg·L-1 IBA. Rootless plantlets inoculated in the medium of 1/2MS+05 mg·L-1 IBA+02 mg·L-1 NAA grew quickly and the growth of adventitious root was good， which were ready for transplanting. The optimal heat treatment time was 10 days which was beneficial for growth and virus elimination of rootless plantlets. After the heat treatment， 100% target viruses in the rootless plantlets were taken off.

Key words: grape, shoot tip, virus elimination, rapid propagation