浙江农业学报

• 食品科学 • 上一篇    下一篇

金雀花总黄酮提取工艺优化及抗氧化性研究

  

  1. (1 楚雄师范学院 化学与生命科学学院,云南 楚雄 675000;2 云南省高校应用生物学重点实验室,云南 楚雄 675000)
  • 出版日期:2015-02-25 发布日期:2015-03-12

Optimization of extraction technology of total flavonoids in Parochetus communis and the antioxidative effects

  1. (1 Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong 675000, China;2 Yunnan Province Universities Key Laboratory of Applied Biology, Chuxiong 675000, China)
  • Online:2015-02-25 Published:2015-03-12

摘要: 采用 Al(NO3)3\|NaNO2比色法测定金雀花总黄酮的含量,通过L9 (34)正交试验优化了总黄酮的提取工艺,并对总黄酮清除羟基自由基和DPPH自由基的作用进行了初步研究。结果表明:最佳提取工艺参数为乙醇体积分数75%,提取温度75℃,料液比1∶40 (g·mL-1),提取时间150 min,在此条件下总黄酮平均含量为1.543 mg·g-1;总黄酮对·OH和DPPH·的清除率分别可达42.59%和60.40%,说明该类物质对·OH和DPPH·有较好的清除能力。

关键词: 金雀花, 总黄酮, 正交试验, 羟基自由基, DPPH自由基

Abstract: In this study, the content of total flavonoids in Parochetus communis was determined using Al(NO3)3\|NaNO2 method. The extraction technology of total flavonoids was optimized through L9 (34) orthogonal experiment. The effect of total flavonoids on scavenging of hydroxyl radicals and DPPH radicals in Parochetus communis was also studied. The results showed that the optimum extraction parameters were as follows: The volume fraction of ethanol was 75%, extraction temperature was 75℃, ratio of solid to liquid was 1∶40 (g·mL-1), and extraction time was 150 min. Under such condition, the average content of total flavonoids was 1.543 mg·g-1, and the scavenging effect of total flavonoids on ·OH and DPPH· were 42.59% and 60.40% respectively, implying such material can effectively scavenge ·OH and DPPH·.

Key words: Parochetus communis, total flavonoids, orthogonal experiment, hydroxyl free radical, DPPH free radical