关键词: 番茄, 黄化曲叶病毒（TYLCV）, 田间鉴定, 苗期接种鉴定, 抗性基因

Abstract: Twenty\|six autogeny lines were selected to assess resistant levels to TYLCV and genotypes by natural incidence in field， seedling artificial inoculation， PCR amplification and TaqⅠrestriction enzyme. Anaizi and Hangza 301 were used as susceptible control and resistant control， respectively. The results showed that 27 tested materials all were high resistant to TYLCV， 1 tested material was susceptible to TYLCV， and the results of identification of natural incidence was consistent with artificial inoculation. According to molecular detection， 26 tested autogeny materials contains dominant Ty gene except Anaizi and No.24 material， 21 of which contain homozygous gene， 5 of which contain heterozygous gene. Ty\|4 and Ty\|5 gene were not detected among tested materials. The coincidence rate reached above 964% among molecular detection， artificial inoculation and natural incidence in field. It was indicated that materials containing dominant Ty showed superior resistant symptoms， while materials containing different resistant genes had different resistant symptoms. Materials carrying two or more resistant genes had higher and more stable resistant level. So combination of molecular detection and field identification could get a material containing two or more resistant genes which might have higher， broader and more stable resistance.

Key words: tomato, Tomato yellow leaf curl virus, field identification, seedling artificially inoculation, resistance gene