关键词: 甜椒, CMV, 一步法RT\, PCR, 抗性鉴定, 生理生化指标

Abstract: In order to elucidate the physiological and biochemical mechanism of sweet peppers infected by Cucumber mosaic virus（CMV）， two different disease\|resistant sweet peppers were used as materials. Firstly， we detected the inoculated virus types by one\|step RT\|PCR， and a 780 bp band was obtained. Then， we studied the changes of electrolytic leakage， malondialdehyde（MDA）content， soluble protein content， the enzymatic activities of superoxide dismutase （SOD）， peroxidase （POD）， catalase（CAT） and phenylalnine ammonialyase（PAL） during 15 days. The results showed that: after CMV inoculation， while the treating time was prolonged， electrolytic leakage and MDA content exhibited an increasing trend as a whole， and the increase in Shenjiaoyihao with stronger resistance was lower than that in P14004 with weaker resistance. The antioxidant enzyme activities of both test plants increased， except that the SOD activity decreased， and the POD and CAT activity in Shenjiaoyihao showed higher increase rate than P12004. The soluble protein content elevated at first and reduced at last， so did the PAL activity in the two sweet pepper varieties， and both of the two indexes showed higher level in Shenjiaoyihao than in P14004.

Key words: sweet pepper（Capsium annuum var. grossum）, CMV, one\, step RT\, PCR, resistance identification, physiological and biochemical index