浙江农业学报

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低温胁迫差异表达基因在佛手和枳中的半定量RT\|PCR分析

  

  1. (1浙江省舟山市定海区农林与海洋渔业局,浙江 舟山 316000;2浙江师范大学 化学与生命科学学院,浙江 金华 321004)
  • 出版日期:2015-12-25 发布日期:2016-01-05

Semi\|quantitative RT\|PCR analysis of cold\|stress induced and differentially expressed genes in fingered citron (Citrus medica var. sarcodactylis Swingle) and Poncirus trifoliata

  1. (1 Dinghai District Agriculture & Forestry and Marine Fisheries Bureau of Zhejiang, Zhoushan 316000, China; 2 College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China)
  • Online:2015-12-25 Published:2016-01-05

摘要: 以佛手(Citrus medica L. var. sarcodactylis Swingle)和枳(Poncirus trifoliata Raf.)为试材,-4 ℃低温处理24 h后,采用半定量RT\|PCR技术,研究佛手34个低温胁迫差异表达基因在佛手和枳中的表达情况,通过比较获得佛手低温敏感相关基因,分析两者抗寒性差异原因。结果表明,佛手和枳中变化趋势不同的基因有17个,其中14个基因在佛手中表达量有变化,枳中表达量不变,3个基因在佛手与枳中表现出完全相反的变化趋势;8个基因变化趋势相同;9个基因在枳中未检测到。推测17个变化趋势不同的基因可能与佛手低温敏感相关,它们所介导的分子途径可为揭示佛手低温敏感提供有力证据;9个枳中未检测到的基因是佛手中特异表达基因,可能是引起佛手低温敏感,造成佛手和枳抗寒性差异的关键基因。

关键词: 佛手;枳;低温胁迫;差异表达基因;半定量RT\, PCR

Abstract: Aimed to investigate cold sensitive genes in fingered citron (Citrus medica var. sarcodactylis Swingle) and unveil the differences of cold resistance mechanism between C.medica and Poncirus trifoliata. The expression patterns of 34 cold\|stress induced genes in C.medica and P.trifoliata were analyzed by semi\|quantitative RT\|PCR after being exposed to -4 ℃ for 24 h. The results showed that the expression levels of 17 genes were different between C.medica and P.trifoliata, among which the transcripts levels of 14 genes changed response to cold\|stress, while no changes were observed in P.trifoliate simultaneously. The transcript levels of 3 genes were found with contrary changing models in two plant species. Besides, the expression of 8 genes in C.medica shared similar pattern as in P.trifoliata, and 9 genes failed to be amplified in P.trifoliata. Thus, it was suggested that the 17 genes differentially expressed in these two plant species were related to cold sensitivity of C.medica. The pathway they were involved in might be the important molecular mechanism regarding cold sensitivity of C.medica. Nine genes with different expression levels induced by cold\|stress in C.medica, which could not be amplified in P.trifoliata, might be the important genes involved in the cold sensibility of C.medica and the important reason resulted in the different cold resistance between C.medica and P.trifoliata.

Key words: fingered citron, Poncirus trifoliata, cold\, stress, differentially expressed genes, semi\, quantitative RT\, PCR