浙江农业学报

• 园艺科学 • 上一篇    下一篇

紫色辣椒叶片花青素的纯化及其生物活性分析

  

  1. (1. 安徽科技学院 农学院,安徽 凤阳 233100;2. 安徽农业大学 生命科学学院,安徽 合肥 230031)
  • 出版日期:2016-03-25 发布日期:2016-04-07

Purification of anthocyanin from purple pepper leaf and its bioactivities

  1. (1. College of Agriculture, Anhui Science and Technology University, Fengyang 233100, China; 2. College of Life Science, Anhui Agricultural University, Hefei 230031, China)
  • Online:2016-03-25 Published:2016-04-07

摘要: 通过正交实验,研究紫色辣椒叶片花青素纯化的影响因素及其精制品的生物活性。结果表明:影响大孔树脂HPD100吸附紫色辣椒叶片花青素的因素大小顺序为A(pH)>C(固液比)>B(温度)>D(转速)。树脂HPD100吸附紫色辣椒叶片花青素的最优组合为A4B3C2D4,而对上述树脂解吸附的乙醇浓度为40%。在抗氧化方面,紫色辣椒叶片花青素精制品清除羟自由基(·OH)的效果要优于Vc,而清除超氧阴离子(O-2·)和DPPH自由基的能力要低于Vc;在抑菌性能方面,紫色辣椒叶片花青素精制品对真菌(根霉、黑曲霉)无抑制效果,对细菌(大肠杆菌、金黄色葡萄球菌、地衣芽孢杆菌等)有一定的抑菌效果,且抑菌效果具有浓度效应。

关键词: 大孔树脂, 紫色辣椒, 抗氧化, 生物活性

Abstract: Factors influencing purification of anthocyanin from purple pepper leaf and bioactivities of the refined products were studied. It was shown that the influence of factors regarding adsorption of purple pepper leaf anthocyanin by HPD100 macroporous resin decreased as A (pH)>C (the ratio of solid to liquid)>B (temperature)>D (speed). The optimal combination for adsorption of purple pepper leaf anthocyanin by HPD100 macroporous resin was A4B3C2D4, and the concentration of ethanol solution for desorption of the above resin was 40%. The ability of scavenging hydroxyl free radical (·OH) by refined leaf anthocyanin from purple pepper was stronger than that of Vc, while abilities of scavenging superoxide anion (O-2·) and DPPH free radicals were weaker. The refined leaf anthocyanin of purple pepper showed no inhibitory effect on fungi (Rhizopus, Aspergillus niger), but exhibited inhibitory effect on bacteria (Escherichia coli, Staphylococcus aureus and Bacillus licheniformis) with concentration effect.

Key words: macroporous resin, purple pepper, antioxidation, bioactivities