高等植物花青素生物合成、调控、生物活性及其检测的研究进展

doi:10.3969/j.issn.1004-1524.20230660

浙江农业学报 ›› 2024, Vol. 36 ›› Issue (4): 978-996.DOI: 10.3969/j.issn.1004-1524.20230660

• 综述 • 上一篇

高等植物花青素生物合成、调控、生物活性及其检测的研究进展

牛钰¹(), 李晶¹, 王俊文¹, 李瑞瑞¹, 田强¹, 武玥¹^,^*(), 郁继华¹^,²^,^*()

1.甘肃农业大学园艺学院,甘肃兰州 730070
2.省部共建干旱生境作物学重点实验室,甘肃兰州 730070

收稿日期:2023-05-18 出版日期:2024-04-25 发布日期:2024-04-29
作者简介:牛钰(1999—),女,甘肃卓尼人,藏族,硕士研究生,研究方向为设施蔬菜栽培生理与生长调控研究。E-mail:2262832454@qq.com
通讯作者: *武玥,E-mail:wuy@gsau.edu.cn;郁继华,E-mail:yujihua@gsau.edu.cn
基金资助:
甘肃省高等学校创新基金项目(2021B-124);“双一流”科研重点项目(GSSYLXM-02);甘肃省拔尖领军人才培养计划(GSBJLJ-2021-14)

Research progress of anthocyanin biosynthesis, regulation, bioactivity and detection in higher plants

NIU Yu¹(), LI Jing¹, WANG Junwen¹, LI Ruirui¹, TIAN Qiang¹, WU Yue¹^,^*(), YU Jihua¹^,²^,^*()

1. College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China
2. State Key Laboratory of Aridland Crop Science, Lanzhou 730070, China

Received:2023-05-18 Online:2024-04-25 Published:2024-04-29
Contact: WU Yue,YU Jihua

摘要/Abstract

摘要：

花青素是广泛存在于植物体中的一种重要次级代谢物,是影响植物呈色的关键物质,其生物合成具有一定的组织表达特异性,并能够受到内源和外源因素的调控,包括转录因子、植物生长调节剂以及环境条件。文章综述了近年来植物花青素的研究进展和现状,对花青素的生物合成、调控网络、影响因素、生物活性和检测策略进行了系统地阐述,并对存在的问题和未来研究方向进行了探讨。

关键词: 花青素, 生物合成途径, 转录因子, 调控机理, 生物活性, 检测方法

Abstract:

Anthocyanin is an important secondary metabolite in higher plants, and is a key substance affects the color of plants. The biosynthesis of anthocyanin has specificity in certain tissue and can be regulated by endogenous and exogenous factors, including transcription factors, plant growth regulators and environmental conditions. In this paper, the research progress and advance of anthocyanin in recent years were reviewed, including the biosynthesis pathway, regulatory network, influenced factors, bioactivity and detection strategies of anthocyanins. Moreover, the existing problems and future research directions were discussed.

Key words: anthocyanin, biosynthetic pathway, transcription factor, regulative mechanism, bioactivity, detection method

中图分类号:

Q945

牛钰, 李晶, 王俊文, 李瑞瑞, 田强, 武玥, 郁继华. 高等植物花青素生物合成、调控、生物活性及其检测的研究进展[J]. 浙江农业学报, 2024, 36(4): 978-996.

NIU Yu, LI Jing, WANG Junwen, LI Ruirui, TIAN Qiang, WU Yue, YU Jihua. Research progress of anthocyanin biosynthesis, regulation, bioactivity and detection in higher plants[J]. Acta Agriculturae Zhejiangensis, 2024, 36(4): 978-996.

图/表 5

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花青素种类 Anthocyanins species	R1碳位官能团 R1 carbon funtional group	R2碳位官能团 R2 carbon funtional group	物种 Plant species	参考文献 References
天竺葵色素 Pelargonidin (Pe)	H	H	桑葚Morus alba L. 玫瑰Rosa spp.	[27-28]
矢车菊色素Cyanidin (Cy)	OH	H	蓝莓Vaccinium spp.	[29]
飞燕草色素 Delphindin (DP)	OH	OH	黑枸杞Lycium ruthenicum Murr. 蓝莓Vaccinium spp.	[30-31]
芍药花色 Peonidin (Pn)	OMe	H	玫瑰Rosa rugosa 黑胡萝卜Daucus carota L.	[32] [28]
牵牛花色素 Petunidin (Pt)	OMe	OH	马铃薯Solanum tuberosum 黑枸杞Lycium ruthenicum Murr.	[33-34]
锦葵色素 Malvidin (Mv)	OMe	OMe	葡萄Vitis vinifera L. 越橘Vaccinium corymbosum	[35-36]

花青素种类 Anthocyanins species	R1碳位官能团 R1 carbon funtional group	R2碳位官能团 R2 carbon funtional group	物种 Plant species	参考文献 References
天竺葵色素 Pelargonidin (Pe)	H	H	桑葚Morus alba L. 玫瑰Rosa spp.	[27-28]
矢车菊色素Cyanidin (Cy)	OH	H	蓝莓Vaccinium spp.	[29]
飞燕草色素 Delphindin (DP)	OH	OH	黑枸杞Lycium ruthenicum Murr. 蓝莓Vaccinium spp.	[30-31]
芍药花色 Peonidin (Pn)	OMe	H	玫瑰Rosa rugosa 黑胡萝卜Daucus carota L.	[32] [28]
牵牛花色素 Petunidin (Pt)	OMe	OH	马铃薯Solanum tuberosum 黑枸杞Lycium ruthenicum Murr.	[33-34]
锦葵色素 Malvidin (Mv)	OMe	OMe	葡萄Vitis vinifera L. 越橘Vaccinium corymbosum	[35-36]

类别 Category	转录因子 Transcription factors	物种 Plant species	调节基因 Regulate genes	参考文献 Reference
MYB	PmMYBa1	梅花Armeniaca mume Sieb.	DFR, ANS	[95]
	EsAN2	淫羊藿Epimedium brevicornu Maxim.	CHIS, CHI, ANS	[96]
	CmMYB6	菊花Dendranthema morifolium Tzvel.	DFR	[97]
	GmMYB10	山竹Garcinia mangostana L.	DFR, UFGT	[79]
	TaMyb1D	烟草Nicotiana tabacum L.	PAL, CHS, CHI, F3H, DFR, FLS	[98]
	OsPL	水稻Oryza sativa L.	PAL, CHS, ANS	[99]
	AtMYBL2	拟南芥Arabidopsis thaliana	AtTT8, DFR	[100]
	TaPL1	小麦Triticum aestivum	PAL, CHS, CHI, F3H, DFR	[37]
	VvMYBA1/6/7	葡萄Vitis vinifera L.	UFGT, 3AT	[101]
	DcMYB6	黑胡萝卜Daucus carota L.	CHS, DFR	[102]
	PpMYB10.1	桃Prunus persica	DFR, UFGT	[103]
	PpMYBPA1	桃Prunus persica	LAR1	[103]
bHLH	PsbHLH1	牡丹Paeonia suffruticosaAndr.	DFR, ANS	[104]
	PubHLH2	石榴Punica granatum L.	DFR, ANS	[105]
	AtGL3/TT8	拟南芥Arabidopsis thaliana	CHS, CHI, F3H, F3'H, DFR, ANS, GT	[106]
	LeAH	番茄Lycopersicon esculentum Mill.	F3'5'H, DFR, ANS, 3GT, GST	[86]
	MdMYC2	苹果Malus×domestica	DFR, UF3GT, F3H, CHS	[107]
	MdbHLH3	苹果Malus×domestica	DFR, UFGT	[108]
	AcbHLH42	猕猴桃Actinidia chinensis Planch	F3GT1, ANS	[87]
	SmTT8	茄子Solanum melongena L.	CHI, F3H, DFR, 3GT, 5GT	[109]
WD40	MdTTG1	苹果Malus×domestica	PAL, CHI, CHS	[76]
	PhAN11	矮牵牛Petunia hybrida Vilm.	DFR	[90]
	Pu TTG1	石榴Punica granatum L.	DFR, LDOX	[105]
	GhTTG1/TTG3	棉花Gossypium spp.	DFR	[110]
	StAN11	马铃薯Solanum tuberosum	DFR	[111]

类别 Category	转录因子 Transcription factors	物种 Plant species	调节基因 Regulate genes	参考文献 Reference
MYB	PmMYBa1	梅花Armeniaca mume Sieb.	DFR, ANS	[95]
	EsAN2	淫羊藿Epimedium brevicornu Maxim.	CHIS, CHI, ANS	[96]
	CmMYB6	菊花Dendranthema morifolium Tzvel.	DFR	[97]
	GmMYB10	山竹Garcinia mangostana L.	DFR, UFGT	[79]
	TaMyb1D	烟草Nicotiana tabacum L.	PAL, CHS, CHI, F3H, DFR, FLS	[98]
	OsPL	水稻Oryza sativa L.	PAL, CHS, ANS	[99]
	AtMYBL2	拟南芥Arabidopsis thaliana	AtTT8, DFR	[100]
	TaPL1	小麦Triticum aestivum	PAL, CHS, CHI, F3H, DFR	[37]
	VvMYBA1/6/7	葡萄Vitis vinifera L.	UFGT, 3AT	[101]
	DcMYB6	黑胡萝卜Daucus carota L.	CHS, DFR	[102]
	PpMYB10.1	桃Prunus persica	DFR, UFGT	[103]
	PpMYBPA1	桃Prunus persica	LAR1	[103]
bHLH	PsbHLH1	牡丹Paeonia suffruticosaAndr.	DFR, ANS	[104]
	PubHLH2	石榴Punica granatum L.	DFR, ANS	[105]
	AtGL3/TT8	拟南芥Arabidopsis thaliana	CHS, CHI, F3H, F3'H, DFR, ANS, GT	[106]
	LeAH	番茄Lycopersicon esculentum Mill.	F3'5'H, DFR, ANS, 3GT, GST	[86]
	MdMYC2	苹果Malus×domestica	DFR, UF3GT, F3H, CHS	[107]
	MdbHLH3	苹果Malus×domestica	DFR, UFGT	[108]
	AcbHLH42	猕猴桃Actinidia chinensis Planch	F3GT1, ANS	[87]
	SmTT8	茄子Solanum melongena L.	CHI, F3H, DFR, 3GT, 5GT	[109]
WD40	MdTTG1	苹果Malus×domestica	PAL, CHI, CHS	[76]
	PhAN11	矮牵牛Petunia hybrida Vilm.	DFR	[90]
	Pu TTG1	石榴Punica granatum L.	DFR, LDOX	[105]
	GhTTG1/TTG3	棉花Gossypium spp.	DFR	[110]
	StAN11	马铃薯Solanum tuberosum	DFR	[111]

物种 Species	测定样品/部位 Measuring sample/parts	方法 Method	参考文献 Reference
葡萄Vitis vinifera L.	果肉Pulp	纤维素薄层色谱-密度分析法Thin-layer chromatography	[166]
葡萄Vitis vinifera L.	果皮Pericarp	高效液相色谱法High-performance liquid chromatography	[167]
越橘Vaccinium dunalianum	叶片Leaf	紫外分光光度计法The UV spectrophotometry	[168]
蓝莓Vaccinium spp.	果实Fruit	酶辅助提取Enzyme-assisted extraction	[169]
黑胡萝卜Daucus carota L.	根Root	pH示差法The pH-differential spectrophotometry	[170]
葡萄Vitis vinifera L.	果皮Pericarp	反向高效液相色谱法	[171]
		Reversed phase high-performance liquid chromatography
苹果Malus×domestica	果实Fruit	对-二甲基氨基肉桂醛法The P-dimethylamino-cinnamaldehyde method	[172]
覆盆子Rubus idaeus L.	果实Fruit	超高效液相色谱-光电二极管阵列检测器-四极杆飞行时间质谱法	[173]
		UPLC-PDA-Q/TOF-MS
巴西莓	冻干巴西莓粉	液相色谱-质谱联用测定法LS-MS	[174]
Euterpe oleracea Mart.	Freeze-dried acaie powder
蔓越莓Oxycoccos	果肉Pulp	香草醛法Vanillin method	[175]
蓝莓Vaccinium spp.	果肉Pulp	电化学发光法Electrochemiluminescence	[176]
甘草	根Root	超高效液相色谱-二极管阵列检测器串联质谱法MS/MS- UPLC-PDA	[177]
Glycyrrhiza uralensis Fisch.
山楂	果肉Pulp	定量核磁共振法Quantitative NMR	[178]
Crataegus pinnatifida Bge.
马铃薯Solanum tuberosum	块茎Tuber	超高效液相色谱-二极管阵列检测器法UPLC-PDA	[179]
黑稻Oryza sativa L.	种子Seeding	欧姆加热辅助萃取Ohmic heating assisted extraction	[180]
马齿苋Portulaca oleracea L.	叶片Leaf	超高效液相色谱-电喷雾串联三重四级杆质谱法UPLC-ESI-MS/MS	[181]

高等植物花青素生物合成、调控、生物活性及其检测的研究进展

Research progress of anthocyanin biosynthesis, regulation, bioactivity and detection in higher plants

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