浙江农业学报

• 园艺科学 • 上一篇    下一篇

基于ISSR分子标记的茶树品系丽早香遗传特性分析

  

  1. (1.浙江省丽水市农业局农作物站,浙江 丽水323000; 2.中国农业科学院 茶叶研究所/国家茶树改良中心,浙江 杭州 310008)
  • 出版日期:2016-06-25 发布日期:2016-06-12

Analysis of genetic characteristics in tea line Lizaoxiang based on ISSR markers

  1. (1. Crop Workstation of Agricultural Bureau of Lishui, Lishui 323000, China; 2. Tea Research Institute of Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Hangzhou 310008, China)
  • Online:2016-06-25 Published:2016-06-12

摘要: 丽早香是浙江省丽水市选育的茶树品系,研究其遗传特性及与其他茶树品种差异性,是申请植物新品种保护和开展新品种审定的重要判定依据。以浙江丽水主栽品种嘉茗1号(乌牛早)、迎霜、龙井43和当地群体种(鸠坑)为比较对象,采用改进的CTAB法提取上述4个品种和丽早香(山地种和大田种)6个材料的总DNA,应用简单重复区间序列(ISSR)标记的方法,分析了丽水本地品系丽早香的遗传特性。结果表明:丽早香在不同栽培环境中基因型没有差异,是一个稳定的品系;而且,丽早香与嘉茗1号(乌牛早)的亲缘关系最近,与龙井43的亲缘关系最远,与迎霜和当地群体种的亲缘关系介于两者之间。

关键词: 丽早香, ISSR, 遗传多样性, 亲缘关系

Abstract: Lizaoxiang is a tea line bred in Lishui City, Zhejiang Province, whose genetic characteristics and diversity with other tea cultivars were the important basis for the examination of new varieties. The local major tea cultivars such as Jiaming No.1(Wuniuzao), Yingshuang, Longjing 43 and Jiukeng were selected as experimental materials, and the total DNA of 6 materials including the above four cultivars and Lizaoxiang(mountain land type and field land type)were extracted using improved CTAB method and the genetic diversity and relationship of Lizaoxiang were analyzed using intersimple sequence repeat (ISSR) markers. The results indicated that Lizaoxiang is a stable tea line without variation in DNA profiles in different cultivation environment. Phylogenetic relationships revealed that Lizaoxiang was closest to Jiaming No.1 (Wuniuzao) but farthest from Longjing 43, while Yingshuang and Jiukeng were in between.

Key words: Lizaoxiang, ISSR, genetic diversity, genetic relationship