关键词: 真菌糖蛋白, 理化性质, 单糖组成, 分子量分布, 顺铂, 恢复作用

Abstract: The glycoprotein obtained from six different edible fungus were extracted by water and alcohol precipitation method, and phenol-sulfuric acid method, hydroxyl biphenyl method and BCA method were used to determine the content of total polysaccharides, uronic acid and protein content of six different edible fungus. The monosaccharide composition and the molecular weights distribution of each sample were determined through PMP pre-column derivatization method and HPGPC method. The renal cell injury model was established by cisplatin (CDDP)-induced Madin-Daiby Canine Kidney (MDCK) cell was to study the restoration effect of six different edible fungus glycoprotein. The results showed that Lentinus edodes glycoprotein contained the highest content of total polysaccharides among the six different edible fungus, which was 38.11%. Pleurotus ostreatus contained the highest content of protein, which was 34.21%, while the contents of uronic acid of each sample was low. Each of the sample contained mannose, glucose and galactose, and its molecular weight distribution ranged from 500-20 000 u. When the concentration was 12.5-50 μg·mL^-1, each sample had restoration effect on renal cell injury by CDDP-induced MDCK cell. The experimental results illustrated that the content of polysaccharides was rich in the six different edible fungus, and had restoration effect on the renal cell injury.

Key words: edible fungus glycoprotein, physical and chemical characteristics, monosaccharide composition, molecular weights distribution, cisplatin, restoration effect