夏季遮荫对月季叶片PSⅡ活性和抗氧化系统的影响

doi:10.3969/j.issn.1004-1524.2016.09.11

浙江农业学报 ›› 2016, Vol. 28 ›› Issue (9): 1530-1537.DOI: 10.3969/j.issn.1004-1524.2016.09.11

夏季遮荫对月季叶片PSⅡ活性和抗氧化系统的影响

侯维, 马旭辉, 张皓月, 陈其阳, 张勇^*

四川农业大学园艺学院, 四川成都 611130

收稿日期:2015-12-30 出版日期:2016-09-15 发布日期:2016-11-23
通讯作者: 张勇,E-mail: zhyong@sicau.edu.cn
作者简介:侯维(1982—),女,四川彭州人,硕士,讲师,从事观赏植物景观应用等研究。E-mail: camellia1216@126.com
基金资助:
四川省教育厅项目(15ZB0002); 四川农业大学学科建设双支计划

Effects of shading on photosystem Ⅱ activity and antioxidant system of Rosa hybrida leaves in summer

HOU Wei, MA Xu-hui, ZHANG Hao-yue, Chen Qi-yang, ZHANG Yong^*

College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China

Received:2015-12-30 Online:2016-09-15 Published:2016-11-23

摘要/Abstract

摘要： 以盆栽3年生月季苗为试材,采用人工遮荫方法研究不同程度遮荫(100%、72%和26%自然光强)对叶片PSⅡ活性和抗氧化系统的影响。结果表明,随着光强的减弱,月季净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)和胞间CO₂浓度(Ci)降低,而气孔限制值(Ls)升高。叶绿素荧光参数Fv/Fm日变化呈单谷曲线,非光化学猝灭系数(NPQ)随光强增加具有升高的趋势。类胡萝卜素(Cars)、类胡萝卜素/叶绿素(Car/Chl)和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性随光强增加而升高,叶绿素(Chl)含量和瞬时光能利用效率(LUE)降低。研究发现,夏季晴天高温强光下,月季叶片PSⅡ反应中心发生了可逆失活,PSⅡ功能下调,光合作用的光抑制明显发生;适度遮荫(72%光强)可减轻光抑制,改善光合功能以增加光合产物积累;过度遮荫(26%光强),月季叶片PSⅡ反应中心受到严重伤害,发生了长期光抑制,月季生长受到抑制。

关键词: 月季, 光合特性, 抗氧化酶, 光抑制

Abstract: Potted 3 years old seedlings of Rosa hybrida were grown in 26%, 72% and 100% natural light intensity, and the effects of light intensity on photosystem Ⅱ activity and antioxidant system of R. hybrida were studied. It had been found that with the decreasing light intensity, the gas exchange parameters such as net photosynthesis rate (Pn), stomatal conduction (Gs), transpiration rate (Tr) and intercellular CO₂ concentration (Ci) all decreased, while the stomatal limitation value (Ls) increased. The maximum efficiency of PSⅡ photochemistry (Fv/Fm) was demonstrated single peak, while fluorescent non-photochemical quenching (NPQ) increased. Carotenoids (Cars) content, ratio of carotenoid to total chlorophyll (Car/Chl) and activities of antioxidant enzymes such as SOD, CAT and APX increased with increasing light intensitiy, while the chlorophyll (Chl) content and light use efficiency (LUE) decreased. There was no photoinhibition in 72% natural light intensity of R. hybrida. While in 100% natural light intensity, its photoinhibition was mainly caused by reversible deactivation of PSⅡ reaction center. In 26% natural light intensity, the reaction center of PSⅡ was seriously destroyed, and this had an photoinhibition, the growth of R. hybrida was also inhibited.

Key words: Rosa hybrida, photosynthetic characteristics, antioxidant enzyme, photoinhibition

中图分类号:

S685.12

侯维, 马旭辉, 张皓月, 陈其阳, 张勇. 夏季遮荫对月季叶片PSⅡ活性和抗氧化系统的影响[J]. 浙江农业学报, 2016, 28(9): 1530-1537.

HOU Wei, MA Xu-hui, ZHANG Hao-yue, Chen Qi-yang, ZHANG Yong. Effects of shading on photosystem Ⅱ activity and antioxidant system of Rosa hybrida leaves in summer[J]. , 2016, 28(9): 1530-1537.

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夏季遮荫对月季叶片PSⅡ活性和抗氧化系统的影响

Effects of shading on photosystem Ⅱ activity and antioxidant system of Rosa hybrida leaves in summer

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