三种浙产铁皮石斛多糖的结构及免疫功效探究

doi:10.3969/j.issn.1004-1524.20221009

浙江农业学报 ›› 2023, Vol. 35 ›› Issue (8): 1888-1895.DOI: 10.3969/j.issn.1004-1524.20221009

三种浙产铁皮石斛多糖的结构及免疫功效探究

李镜锐¹(), 陶文扬², 杨颖², 周万怡², 陆胜民²^,^*(), 王阳光¹^,^*()

1.浙江海洋大学食品与药学学院,浙江舟山 316022
2.浙江省农业科学院食品科学研究所,浙江杭州 310021

收稿日期:2022-07-07 出版日期:2023-08-25 发布日期:2023-08-29
作者简介:李镜锐(1997—),女,黑龙江大庆人,硕士研究生,研究方向为铁皮石斛多糖功效。E-mail:877613266@qq.com
通讯作者: *陆胜民,E-mail: lushengmin@163.com;王阳光,E-mail: 634449563@qq.com
基金资助:
浙江省重点研发计划项目(2020C02034)

Comparative studies of polysaccharides of three Dendrobium species from Zhejiang Province and exploration of their physiological functions

LI Jingrui¹(), TAO Wenyang², YANG Ying², ZHOU Wanyi², LU Shengmin²^,^*(), WANG Yangguang¹^,^*()

1. College of Food and Medicine, Zhejiang Ocean University, Zhoushan 316022, Zhejiang, China
2. Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2022-07-07 Online:2023-08-25 Published:2023-08-29

摘要/Abstract

摘要：

为比较3种来自雁荡山产业基地的铁皮石斛茎多糖的基本理化性质及免疫功效,为其精准利用提供参考,本文对雁斛1号石斛茎多糖(DOP1)、雁斛3号石斛茎多糖(DOP2)和圣兰8号石斛茎多糖(DOP3)进行提取,用高效液相色谱法(HPLC)、苯酚硫酸法、高效凝胶渗透色谱法(HPGPC)和红外光谱法(FT-IR)对其单糖组成、总糖含量、分子量、单糖连接方式进行比较,并探究了其对免疫功能的影响。结果表明,3种多糖均含有3个不同组分,且均由阿拉伯糖、半乳糖、葡萄糖和甘露糖4种单糖组成,但其单糖比例不同。红外光谱是以β构型为主的吡喃杂多糖。3种多糖均对RAW 264.7巨噬细胞有一定的增殖作用,但DOP3多糖的效果最好(P<0.000 1),且1 600 μg·mL^-1 DOP3能显著促进RAW 264.7细胞NO的释放(P<0.05)。3种多糖的理化性质和免疫功效存在明显差异,而DOP3的免疫调节活性优于其他两种石斛多糖,其原因可能是由3种多糖的结构、组成等差异导致的。

关键词: 铁皮石斛, 多糖, 分子量, 单糖组成, 免疫调节

Abstract:

To comparatively study the basic physicochemical properties and immunological efficacy of the three Dendrobium officinale(D. officinale) stems polysaccharides from the industrial base of Yandang Mountain, and to provide reference for their precise utilization, in this paper, polysaccharides from D. officinale cv. Yanhu 1 (DOP1), D. officinale cv. Yanhu 3 (DOP2) and D. officinale cv. Shenglan 8 (DOP3) were extracted. Their monosaccharide composition, total sugar content, molecular weight and monosaccharide linkages were respectively analyzed by high performance liquid chromatography (HPLC), phenol-sulfuric acid method, high performance gel permeation chromatography (HPGPC) and fourier transform infrared spectrometer (FT-IR). Their effects on immune function were further investigated. The results showed that all the three polysaccharides contained three polysaccharide fractions with different relative molecular weights, and DOP2 having the highest relative molecular weights. All the three DOP consisted of four monosaccharides, including arabinose, galactose, glucose and mannose, but they had different monosaccharide ratios. Their results of FT-IR spectra were almost the same, which showed the presence of pyran heteropolysaccharides with a predominantly β-configuration. All the three polysaccharides had certain proliferative effects on RAW 264.7 cells and the DOP3 was the best (P<0.000 1). In addition, DOP3 (1 600 μg·mL^-1) significantly promoted the release of NO from RAW 264.7 cells (P<0.05). The results indicated that there were significant differences in the physicochemical properties and immune efficacy of the three polysaccharides, and the immunomodulatory activity of DOP3 was superior to the other two Dendrobium polysaccharides, which might be related to their differences in structure and composition.

Key words: Dendrobium officinale, polysaccharide, molecular weight, monosaccharide composition, immunomodulation

中图分类号:

S567

李镜锐, 陶文扬, 杨颖, 周万怡, 陆胜民, 王阳光. 三种浙产铁皮石斛多糖的结构及免疫功效探究[J]. 浙江农业学报, 2023, 35(8): 1888-1895.

LI Jingrui, TAO Wenyang, YANG Ying, ZHOU Wanyi, LU Shengmin, WANG Yangguang. Comparative studies of polysaccharides of three Dendrobium species from Zhejiang Province and exploration of their physiological functions[J]. Acta Agriculturae Zhejiangensis, 2023, 35(8): 1888-1895.

图/表 6

图1 三种石斛茎多糖DOP1、DOP2和DOP3的单糖组成 1,Ara;2,Gal;3,Glc;4,Man.

Fig.1 The monosaccharide composition of three polysaccharides from Dendrobium stem including DOP1, DOP2 and DOP3

表1 DOP总糖含量

Table 1 Total sugar content in DOP

指标Index	DOP1	DOP2	DOP3
Glc含量 Glc content/%	36.23 ±0.69	50.43 ±0.35	32.59 ±0.58
Man含量 Man content/%	55.46 ±0.51	44.35 ±0.49	51.17 ±0.62
总糖含量 Total sugar content/%	91.69 ±1.20	94.78 ±0.84	83.76 ±1.20

图2 三种石斛茎多糖DOP1、DOP2和DOP3高效凝胶渗透色谱图

Fig.2 High performance gel permeation chromatogram of three polysaccharides from Dendrobium stem including DOP1, DOP2 and DOP3

图3 三种石斛茎多糖DOP1 (a)、DOP2 (b)和DOP3 (c)的红外光谱图

Fig.3 FT-IR spectra of three polysaccharides from Dendrobium stem including DOP1 (a), DOP2 (b) and DOP3 (c)

图4 三种石斛茎多糖DOP1 (a)、DOP2 (b)和DOP3 (c)对RAW 264.7细胞增殖作用的影响 ****,P<0.000 1。

Fig.4 Effects of three polysaccharides from Dendrobium stem including DOP1 (a), DOP2 (b) and DOP3 (c) on proliferations rate in RAW 264.7 cell

图5 三种石斛茎多糖DOP1 (a)、DOP2 (b)和DOP3 (c)对RAW 264.7细胞NO释放的影响 *,P<0.05。

Fig.5 Effect of three polysaccharides from Dendrobium stem including DOP1 (a), DOP2 (b) and DOP3 (c) on NO release from RAW 264.7 cells

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三种浙产铁皮石斛多糖的结构及免疫功效探究

Comparative studies of polysaccharides of three Dendrobium species from Zhejiang Province and exploration of their physiological functions

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