浙南小麦核心育种亲本高分子量谷蛋白亚基组成分析

doi:10.3969/j.issn.1004-1524.2018.12.03

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (12): 2001-2006.DOI: 10.3969/j.issn.1004-1524.2018.12.03

浙南小麦核心育种亲本高分子量谷蛋白亚基组成分析

刘永安^1,2, 潘彬荣^1,2, 岳高红^1,2, 梅喜雪², 许立奎^1,2,*, 张宗宸^1,2, 周志辉^1,2

1.浙南作物育种重点实验室, 浙江温州 325006;
2.温州市农业科学研究院作物研究所,浙江温州 325006

收稿日期:2018-03-29 出版日期:2018-12-25 发布日期:2018-12-28
通讯作者: 许立奎,E-mail: lkxu@163.com
作者简介:刘永安(1980—),男,河南太康人,博士,副教授,主要从事作物遗传育种研究。E-mail: liuanliuan123@163.com
基金资助:
“十三五”浙江省农业(粮食)新品种选育重大科技专项(2016C02050-9-8);温州市种子种苗科技创新专项(Z20160004)

HMW-GS composition analysis of core wheat breeding parents in Southern Zhejiang region

LIU Yong'an^1,2, PAN Binrong^1,2, YUE Gaohong^1,2, MEI Xixue^1,2, XU Likui^1,2,*, ZHANG Zongchen^1,2, ZHOU Zhihui^1,2

1. Key Laboratory of Crop Breeding South Zhejiang, Wenzhou 325006, China;
2. Crop Research Institute, Wenzhou Academy of Agricultural Science, Wenzhou 325006, China

Received:2018-03-29 Online:2018-12-25 Published:2018-12-28

摘要/Abstract

摘要： 为了解浙南小麦核心育种亲本的品质遗传基础,采用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)技术对28份核心品种(系)的高分子量谷蛋白亚基(HMW-GS)组成进行分析。结果表明:供试品种(系)在Glu-A1位点具有Null(57.14%)和1(42.86%)2种类型;在Glu-B1位点具有7+8(67.86%)、7+9(25.00%)、17+18(3.57%)和13+16(3.57%)4种类型;在Glu-D1位点具有2+12(96.43%)和5+10(3.57%)2种类型。另外,各供试品种(系)共有5种亚基组合,依次为1/7+8/2+12(39.29%)、Null/7+8/2+12(28.57%)、Null/7+9/2+12(25.00%)、1/17+18/5+10(3.57%)和Null/13+16/2+12(3.57%)。

关键词: 小麦, 浙南, 高分子量谷蛋白亚基

Abstract: In order to learn the genetic basis of quality of the core wheat breeding parents in Southern Zhejiang region, the composition of high molecular weight glutenin subunit of twenty-eight core cultivars (lines) was analyzed using the method of SDS-PAGE. The results showed that there were Null (57.14%) and 1 (42.86%) subunit alleles on Glu-A1 loci; 7+8 (67.86%), 7+9 (25.00%), 17+18 (3.57%) and 13+16 (3.57%) subunit alleles on Glu-B1 loci; 2+12 (96.43%) and 5+10 (3.57%) subunit alleles on Glu-D1 loci. On the other hand, there were five kind of HMW-GS combinations, 1/7+8/2+12 (39.29%), Null/7+8/2+12 (28.57%), Null/7+9/2+12 (25.00%), 1/17+18/5+10 (3.57%) and Null/13+16/2+12 (3.57%), respectively.

Key words: wheat, Southern Zhejiang region, HMW-GS

中图分类号:

S512

刘永安, 潘彬荣, 岳高红, 梅喜雪, 许立奎, 张宗宸, 周志辉. 浙南小麦核心育种亲本高分子量谷蛋白亚基组成分析[J]. 浙江农业学报, 2018, 30(12): 2001-2006.

LIU Yong'an, PAN Binrong, YUE Gaohong, MEI Xixue, XU Likui, ZHANG Zongchen, ZHOU Zhihui. HMW-GS composition analysis of core wheat breeding parents in Southern Zhejiang region[J]. , 2018, 30(12): 2001-2006.

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浙南小麦核心育种亲本高分子量谷蛋白亚基组成分析

HMW-GS composition analysis of core wheat breeding parents in Southern Zhejiang region

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