一株真菌的发酵液对番茄抗盐的促进作用及菌株初步鉴定

doi:10.3969/j.issn.1004-1524.2017.04.13

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (4): 605-610.DOI: 10.3969/j.issn.1004-1524.2017.04.13

一株真菌的发酵液对番茄抗盐的促进作用及菌株初步鉴定

刘国丽¹, 龚娜¹, 陈珣¹, 王娜^{2, *}

1.辽宁省农业科学院微生物工程中心,辽宁沈阳 110161;
2.辽宁省农业科学院植物营养与环境资源研究所,辽宁沈阳 110161

收稿日期:2016-12-21 出版日期:2017-04-20 发布日期:2017-04-27
通讯作者: 王娜,E-mail:wnsxh1999@126.com
作者简介:刘国丽(1986—),女,山东菏泽人,硕士,助理实习员,从事植物内生菌方向研究。E-mail:liugl12@163.com
基金资助:
辽宁省自然科学基金项目(2014027026); 辽宁省科学事业公益研究基金项目(2015002006)

Promotion effect of fungus DL12 fermentation liquid on salt-resistance ability of tomato and primary identification of strain

LIU Guoli¹, GONG Na¹, CHEN Xun¹, WANG Na^{2, *}

1.Center of Microbial Engineering, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China;
2. Institute of Plant Nutrients and Environmental Resources, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China

Received:2016-12-21 Online:2017-04-20 Published:2017-04-27

摘要/Abstract

摘要： 为筛选获得能提高番茄耐盐性的真菌发酵液,试验采用真菌的发酵液处理75 mmol·L^-1 Ca(NO₃)₂ 胁迫下的番茄种子,通过测定种子发芽率、发芽势,选择对种子发芽有促进作用的菌株发酵液,研究其对Ca(NO₃)₂处理下番茄幼苗根干质量、苗干质量等农艺性状指标及植株内源激素等的影响,结果显示,其中部分菌株发酵液能不同程度地提高番茄种子发芽率和发芽势,并能提高根干质量、苗干质量;结合种子发芽指标及幼苗干质量和内源激素含量来看,其中菌株DL12发酵液处理综合效果最好,种子发芽率、发芽势、株高、根干质量、苗干质量较对照分别提升了29%、200%、17.6%、26.8%、18.9%。DL12处理后叶片中IAA含量是对照组的49.6倍。采用真菌通用引物ITS1和ITS2扩增菌株DL12 18S rDNA序列,并与GenBank已知序列比对后,结果显示,菌株DL12与Schizophyllum commune相似度达到99%,推测菌株DL12是Schizophyllum属真菌。

关键词: 番茄, 抗盐, 真菌, 发酵液

Abstract: In order to screen fungi that could improve the salt-resistance ability of Lycopersicon esculentum, firstly, the fermentation liquid of different fungi were used to treat the seeds of Lycopersicon esculentum under 75 mmol·L^-1 Ca(NO₃)₂ stress. Then the seedlings were sprayed by fermentation liquid of different fungi which had good effects on germination of seeds through evaluation of germination rate and germination potential. The plant height and biomass of seedlings after treatment were recorded. Effect of fermentation liquid from strains DL2, DL12, DL15, DWJ2, DWR3 on endogenous hormones content of seedlings were also measured through HPCE chromatograms. Results showed that several fungi strains could partially improve germination rate and germination potential of seeds, and increase the dry weight. DL12 had the best effects among all the fungi. The germination rate, germination potential of seeds, plant height, root dry weight, and seedling dry weight were increased by 29%, 200%, 17.6%, 26.8%, 18.9%, respectively, compared with the control group. The IAA content was 49.6 times of the control group. The fungus general primers ITS1 and ITS2 were used to amplify 18S rDNA sequences of strain DL12, and alignment with the known sequences from GenBank was carried out, results showed that the similarity between strain DL12 and Schizophyllum commune was 99%. It was concluded that DL12 may belong to Schizophyllum sp.

Key words: tomato, salt-resistance, fungus, fermentation liquid

中图分类号:

S182

刘国丽, 龚娜, 陈珣, 王娜. 一株真菌的发酵液对番茄抗盐的促进作用及菌株初步鉴定[J]. 浙江农业学报, 2017, 29(4): 605-610.

LIU Guoli, GONG Na, CHEN Xun, WANG Na. Promotion effect of fungus DL12 fermentation liquid on salt-resistance ability of tomato and primary identification of strain[J]. , 2017, 29(4): 605-610.

参考文献

[1] 顾京晏,顾卫,张化,等.我国设施农业土壤次生盐渍化生物改良措施研究进展[J].北京师范大学学报(自然科学版),2016,52(1):70-75.
GU J Y, GU W, ZHANG H, et al. Biological measures for soil improvement of facility agriculture in China[J]. Journal of Beijing Normal University ( Nature Science ), 2016,52(1):70-75.(in Chinese with English abstract)
[2] 王学征.设施环境盐胁迫对番茄生长发育及膜系统影响的研究[D].哈尔滨:东北农业大学,2004.
WANG X Z. Studies on effects of salt stress on growth and development and membranes system of tomato in protected farm[D].Harbin: Northeast Agricultural University. 2004. (in Chinese with English abstract)
[3] PARIDA A K, DAS A B. Salt tolerance and salinity effects on plants: a review.[J]. Ecotoxicology and Environmental Safety , 2005, 60(3):324.
[4] 顾金凤.微生物菌肥对盐渍化土壤的改良研究[D].扬州:扬州大学,2013.
GU J F. Effects of microbiological fertilizer on salinized soil [D].Yangzhou: Yangzhou University, 2013. (in Chinese with English abstract)
[5] 黄红艳.次生盐渍化土壤的微生物多样性及微生物改良效应研究[D].上海:上海交通大学,2012.
HUANG H Y. Soil microbial diversity in secondary salinization soils and it’s microbial remidation [D]. Shanghai: Shanghai Jiao Tong University, 2012. (in Chinese with English abstract)
[6] 马晓颖, 贾东贝, 李冰宇,等. 利用高效毛细管电泳测定真菌代谢产物吲哚乙酸含量[J].江苏农业科学, 2011, 39(3):439-441.
MA X Y, JIA D B, LI B Y, et al. Content determination of IAA in metabolic of fungi using high-performance capillary electrophoresis [J]. Jiangsu Agricultural Sciences , 2011, 39(3):439-441. (in Chinese with English abstract)
[7] 陶龙兴.内源IAA对亚种间杂交稻籽粒灌浆的信息效应[D].北京:中国农业科学院,2003.
TAO L X. Signaling effects of endogenous IAA on grain filling of subspecies hybrid rice[D]. Beijing: Chinese Academy of Agricultural Sciences, 2003. (in Chinese with English abstract)
[8] 尹培培, 包日双, 戴佳锟,等. 植物激素对植物器官发生影响的研究进展[J].江西农业学报,2012,24(6):37-41.
YIN P P, BAO R S, DAI J K, et al. Research advance in impact of phytohormone on plant organogenesis[J]. Acta Agriculturae Jiangxi , 2012, 24(6):37-41. (in Chinese with English abstract)
[9] 辛树权,王贵,高扬.植物生长促生菌对盐胁迫下水稻种子萌发及幼苗生长的影响[J]. 湖北农业科学,2012,51(3):490-496.
XIN S Q, WANG G, GAO Y. Effects of plant growth-promoting rhizobacteria (PGPR) on the rice germination and seedling development under salt stress [J]. Hubei Agricultural Sciences , 2012, 51(3):490-496. (in Chinese with English abstract)
[10] 郑元元, 岳海涛, 石在强, 等.盐胁迫下解盐促生细菌Rs-5和Rs-198促进棉花种子发芽的机理探讨[J].中国农业科学,2008,41(5):1326-1332.
ZHENG Y Y,YUE H T, SHI Z Q, et al. Physiochemical characters and ability to promote cotton germination of bacteria strains Rs-5 and Rs-198 under salt stress [J]. Scientia Agricultura Sinica , 2008, 41(5):1326-1332. (in Chinese with English abstract)
[11] 罗欢. 芽孢杆菌对植物的促生和耐盐作用及其相关机制的研究[D]. 南京:南京农业大学, 2013.
LUO H. Study of growth promoting and salt tolerance of Bacillus spp. on plant and the research of underlying mechanisms[D].Nanjing: Nanjing Agricultural University,2013. (in Chinese with English abstract)
[12] 吉云秀,黄晓东.植物促生菌对燕麦初生苗盐分胁迫下的促生效应[J].大连海事大学学报, 2007,33(2):86-89.
JI Y X, HUANG X D. Effects of plant growth-promoting rhizobacteria on the growth of oat seedlings under salt stress[J]. Journal of Dalian Maritime University , 2007, 33(2):86-89. (in Chinese with English abstract)
[13] 马晓敏,张红娟,顾沐宇,等. 促生菌的分离鉴定及其对柳枝稷种子在盐胁迫下的萌发促进作用[J].家畜生态学报,2015, 36(6):46-52.
MA X M, ZHANG H J, GU M Y, et al. Isolation and identification of plant growth-promoting Rhizobacteria(PGPR) and its promoting effect on switchgrass seed under NaCl salt stress[J]. Acta Ecologiae Animalis Domastici , 2015, 36(6):46-52. (in Chinese with English abstract)
[14] 王斌. 丛枝菌根真菌提高加工番茄抗盐和抗旱效能及其机理研究[D].石河子:石河子大学,2013.
WANG B. Arbuscular mycorrhizal fungi improve salt and drought resistance to processing tomato and study of their mechanisms [D]. Shihezi: Shihezi University, 2013. (in Chinese with English abstract)
[15] 李娇. 碱蓬内生菌对Na 2 CO 3 胁迫下水稻幼苗生长的缓解作用[D].沈阳:沈阳师范大学,2014.
LI J. Effect of endophyte infection alleviate growth damage of rice seedlings under Na 2 CO 3 stress [D]. Shenyang: Shenyang Normal University, 2014. (in Chinese with English abstract)
[16] 李莎.外生菌根真菌和荧光假单胞菌对油松生长和抗病性的影响[D].杨凌:西北农林科技大学,2011.
LI S. Effects of ectomycorrhiza fungi and Pseudomonas fluorescens to Pinus tabulaeformis growth and disease resistance[D]. Yangling: Northwest A&F University, 2011. (in Chinese with English abstract)

一株真菌的发酵液对番茄抗盐的促进作用及菌株初步鉴定

Promotion effect of fungus DL12 fermentation liquid on salt-resistance ability of tomato and primary identification of strain

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王同林, 叶红霞, 郑积荣, 李明. 番茄果实中主要风味物质研究进展[J]. 浙江农业学报, 2020, 32(8): 1513-1522.
[2]	张亮, 李玉婷, 许晓风. 锰离子胁迫下外生菌根真菌对土壤钾释放的影响[J]. 浙江农业学报, 2020, 32(7): 1215-1222.
[3]	陈乾丽, 汪汉成, 梁永进, 蔡刘体, 黄宇, 周浩, 李忠, 韩洁. 烤后健康烟叶和霉烂烟叶真菌群落结构分析[J]. 浙江农业学报, 2020, 32(6): 1019-1028.
[4]	乔亚丽, 郁继华, 李旺雄, 金宁, 金莉, 吕剑, 肖雪梅, 唐中褀, 胡琳莉. 番茄果实不同采收时间矿质元素变化分析[J]. 浙江农业学报, 2020, 32(3): 430-436.
[5]	刘新宇, 陈鹏, 张光辉, 赵俊杰, 李博浩, 张南, 孙韦珂, 许娟娟, 叶行涛, 魏金鹏, 于高波. 外源脯氨酸对番茄体内残留百菌清降解的调控作用[J]. 浙江农业学报, 2020, 32(3): 437-446.
[6]	张芳, 王佩欣, 何勇, 骆慧枫, 寿国忠. 基于物联网的阳台微型温室作物生长环境因子探究[J]. 浙江农业学报, 2020, 32(2): 234-242.
[7]	李乐, 田敏娇, 高艳明, 李建设. 硒肥对基质培番茄生长和矿质元素积累的影响[J]. 浙江农业学报, 2020, 32(2): 253-261.
[8]	朱晓林, 魏小红, 王宝强, 王贤, 张明君. c-GMP诱导对盐胁迫下番茄的转录组分析[J]. 浙江农业学报, 2020, 32(10): 1788-1797.
[9]	陶晶, 邬奇峰, 石江, 李松昊, 葛江飞, 陈俊辉, 徐秋芳, 梁辰飞, 秦华. 间作与接种丛枝菌根真菌对新垦山地玉米产量和土壤肥力的影响[J]. 浙江农业学报, 2020, 32(1): 115-123.
[10]	胡力文, 张勇, 石真, 符杰, 孙伟, 邓俊良, 任志华, 左之才, 曹随忠, 胡延春. 紫茎泽兰甲醇提取物对石膏样小孢子菌的抑制作用及其机制[J]. 浙江农业学报, 2019, 31(9): 1555-1562.
[11]	孙德智, 杨恒山, 张庆国, 范富, 苏雅乐其其格, 彭靖, 韩晓日. 外源一氧化氮供体硝普钠对番茄幼苗盐胁迫伤害的缓解作用[J]. 浙江农业学报, 2019, 31(8): 1286-1294.
[12]	刘珊珊, 韦鑫, 盛福瑞, 乔清华. 棉花不同发育时期根际微生物的动态变化[J]. 浙江农业学报, 2019, 31(8): 1361-1371.
[13]	杨亚娜, 樊小雪, 徐刚, 张宇, 李亚灵, 温祥珍. 不同红蓝LED光照强度和灌溉量交互作用对番茄幼苗生长的影响[J]. 浙江农业学报, 2019, 31(5): 737-745.
[14]	林薇, 周海霞, 兰挚谦, 张凯歌, 刘吉青, 张雪艳. 基于葵花杆硫酸铵生物基肥的番茄不同生育期配方肥的效果[J]. 浙江农业学报, 2019, 31(5): 756-765.
[15]	邢鲲, 曹俊宇, 赵飞. 设施番茄昆虫群落组成及时间动态的聚类分析[J]. 浙江农业学报, 2019, 31(4): 600-606.