大熊猫阴道源乳酸杆菌生物学特性研究

doi:10.3969/j.issn.1004-1524.2017.07.06

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (7): 1093-1102.DOI: 10.3969/j.issn.1004-1524.2017.07.06

大熊猫阴道源乳酸杆菌生物学特性研究

马晓平¹, 杨天意¹, 俞演¹, 张志和^{2, *}, 王承东³, 古玉^{4, *}

1.四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川成都611130;
2.成都大熊猫繁育研究基地,四川成都610000;
3.中国保护大熊猫研究中心,四川雅安 625000;
4.四川农业大学生命科学院,四川雅安 625014

收稿日期:2016-07-25 出版日期:2017-07-20 发布日期:2017-07-24
通讯作者: 张志和,E-mail:pandaking1888@126.com;古玉,E-mail:guyu632@sicau.edu.cn
作者简介:马晓平(1976—),男,土家族,重庆石柱人,博士,副教授,从事临床兽医学的教学科研工作。E-mail:mxp886@sina.com.cn
基金资助:
国家科技支撑计划项目(2012BAC01B06); 四川省科技厅基础研究计划项目(2013JY0175); 大熊国际资金项目(AD1415)

Study on biological characteristics of Lactobacillus from giant panda vagina

MA Xiaoping¹, YANG Tianyi¹, ZHANG Zhihe^{1, *}, YU Yan¹, WANG Chengdong³, GU Yu^{4, *}

1. Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. Chengdu Research Base of Giant Panda Breeding, Chengdu 610000, China;
3. China Conservation and Research Center for the Giant Panda, Ya??an 625000, China;
4. College of Life Science, Sichuan Agricultural University, Ya??an 625014, China

Received:2016-07-25 Online:2017-07-20 Published:2017-07-24

摘要/Abstract

摘要： 用大熊猫阴道源的3株乳酸杆菌(Lactobacillus salivarius GPV01、Lactobacillus plantarum GPV02和Lactobacillus saerimneri GPV03)作为试验组,用人阴道源3株乳酸杆菌(Lactobacillus acidophilus WAV01、Lactobacillus crispatus WAV02和Lactobacillus plantarum WAV03)作为对照组,进行产过氧化氢能力、耐酸性、耐热性、抗菌活性、药物敏感性及黏附性研究。结果表明:在相同的培养条件下,L. salivarius GPV01、L. plantarum GPV02和L. saerimneri GPV03的产H₂O₂能力和耐热能力不及L. acidophilus WAV01、L. mcrispatus WAV02和L. plantarum WAV03。L. salivarius GPV01、L. plantarum GPV02和L. plantarum WAV03具有很好的酸耐性,在培养基pH为2~3之间时,3株菌的D值在0.2~0.55之间,其中L. plantarum GPV02的D值最高,达到0.55。同时,6株乳酸杆菌的培养上清液对大肠埃希菌、金黄色葡萄球菌和溶血性链球菌均有不同程度的抑制作用,6株乳酸杆菌对3种致病菌的抑制作用远弱于pH 3.5的乳酸。6株乳酸杆菌对多西环素、氨苄西林、头孢曲松、头孢噻肟、阿莫西林高度敏感,而对四环素和利福平一般敏感。6株乳酸杆菌对链霉素、氧氟沙星、庆大霉素、复方新诺明、环丙沙星、磺胺甲基异恶唑表现出耐药。6株乳酸杆菌均能黏附到Hela细胞上,GPV01、03,WAV01、03对 Hela细胞的平均黏附数为18.8~32.8 ·cell^-1。 WAV02黏附能力最强(54.75±2.40)个·cell^-1,而GPV02黏附能力最弱(6.83±0.33)·cell^-1。

关键词: 大熊猫, 乳酸杆菌, 生物学特性

Abstract: In the present study, 3 Lactobacillus strains of giant panda vagina (Lactobacillus salivarius GPV01, Lactobacillus plantarum GPV02 and Lactobacillus saerimneri GPV03) were used as experimental group, and 3 Lactobacillus strains of woman vagina (Lactobacillus acidophilus WAV01, Lactobacillus crispatus WAV02 and Lactobacillus plantarum WAV03) were used as control group to explore the difference of these strains in hydrogen peroxide production capacity, acid resistance, heat resistance, antibacterial activity, drug sensitivity and adhesiveness. It was shown that L. acidophilus WAV01, L. crispatus WAV02 and L. plantarum WAV03 possessed higher H₂O₂-producing ability and heat-resistance than L. salivarius GPV01, L. plantarum GPV02 and L. saerimneri GPV03 under the same culture condition. L. salivarius GPV01, L. plantarum GPV02 and L. plantarum WAV03 exhibited acid tolerance. D values of Lactobacillus suspensions were all between 0.2 to 0.55 for these strains when the pH was between 2 to 3 in the culture medium, and among these strains, L. plantarum GPV02 showed the highest D value (D=0.55) under the above condition. Six culture supernatants of the Lactobacillus strains had different inhibitory effects on Escherichia coli, Staphylococcus aureus and Hemolytic streptococcus. Six Lactobacillus strains showed weaker inhibition on three pathogenic bacteria than lactic acid (pH 3.5). Six Lactobacillus strains were highly sensitive to doxycycline, ampicillin, ceftriaxone, cefotaxime, amoxicillin, and were sensitive to tetracycline and rifampicin. Six Lactobacillus strains showed resistance to streptomycin, ofloxacin, gentamycin, selectrin, ciprofloxacin and sulfamethoxazole. Six Lactobacillus strains could stick on Hela, and the average adhesion number of GPV01, GPV03, WAV01 and WAV03 on each Hela cell was 18.8-32.8. WAV02 had the strongest adhesion ability (54.75±2.40)·cell^-1, while GPV02 had the worst adhesion ability (6.83±0.33)·cell^-1.

Key words: giant panda, Lactobacillus, biological characteristics

中图分类号:

马晓平, 杨天意, 俞演, 张志和, 王承东, 古玉. 大熊猫阴道源乳酸杆菌生物学特性研究[J]. 浙江农业学报, 2017, 29(7): 1093-1102.

MA Xiaoping, YANG Tianyi, ZHANG Zhihe, YU Yan, WANG Chengdong, GU Yu. Study on biological characteristics of Lactobacillus from giant panda vagina[J]. , 2017, 29(7): 1093-1102.

参考文献

[1] 李兰娟. 感染微生态学[M]. 北京:人民卫生出版社,2002.
[2] 曾晶, 卢芳国. 阴道乳酸杆菌的研究进展[J]. 岳阳职业技术学院学报,2009 (5):96-98.
ZENG J, LU F G. The research progress of vaginal lactobacilli [J]. Journal of Yueyang Vocational Technical College . 2009(5):96-98.(in Chinese)
[3] 杨景云. 医用微生态学 [M]. 昆明:中国医学科技出版社,1996.
[4] 马晓平. 大熊猫阴道微生物多样性及部分真菌生物学特性研究 [D]. 南京:南京农业大学,2014.
MA X P. The giant panda vaginal microbial diversity and some fungi biological characteristics research [D]. Nanjing: Nanjing Agricultural University, 2014. (in Chinese with English abstract)
[5] 王丽莉, 陈其御. 产H 2 O 2 乳酸杆菌对大肠杆菌的体外拮抗作用[J]. 基础医学与临床,2006, 26(1):88-91.
WANG L L, CHEN Q Y. Antagonistic activity of the H 2 O 2 + Lactobacilli against E .coli in vitro [J]. Basic & Clinical Medicine . 2006, 26(1):88-91. (in Chinese with English abstract)
[6] KLEBANOFF S J, HILLIER S L, ESCHENBACH D A, et al. Control of the microbial flora of the vagina by H 2 O 2 -generating lactobacilli [J]. Journal Infection Disease , 1991, 164(1):94-100.
[7] 周晓梅, 胡晓蓉, 伍丽群,等. 孕妇阴道乳酸杆菌种属鉴别及过氧化氢产量与妊娠结局的关系[J]. 中国实用医药, 2008, 3(1):14-16.
ZHOU X M, HU X R, WU L Q, et al.Identification and H 2 O 2 production of vaginal Lactobacilli from pregnant women and relation with outcome[J]. China Practical Medcine , 2008, 3(1):14-16. (in Chinese with English abstract)
[8] 谢菲, 常青, 史常旭. H 2 O 2 阳性乳酸杆菌与细菌性阴道病及不良妊娠结局关系的研究[J]. 中国误诊学杂志,2007, 7(10):2181-2183.
XIE F, CHANG Q, SHI C X. Identification of H 2 O 2 -positive vaginal Lactobacilli from pregnant women and relat ion with adverse pregnancy outcomes[J]. Chinese Journal of Misdiagnostics . 2007, 7(10):2181-2183. (in Chinese with English abstract)
[9] VOLTAN S, MARTINES D, ELLI M, et al. Lactobacillus crispatus M247-derived H 2 O 2 acts as a signal transducing molecule activating peroxisome proliferator activated receptor-gamma in the intestinal mucosa[J]. Gastroenterology . 2008, 135(4):1216-1227.
[10] OTERO M C, NADER-MACIAS M E. Inhibition of staphylococcus aureus by H 2 O 2 -producing Lactobacillus gasseri isolated from the vaginal tract of cattle[J]. Animal Reproduction Science . 2006, 96(1/2):35-46.
[11] 周倩如, 刘艳, 刘祥, 等. 阴道内乳酸杆菌抑菌能力相关因素的研究[J]. 卫生研究,2006,35(3):310-313.
ZHOU Q R, LIU Y, LIU X, et al. Studies of the factors linked to the bacteriostatic ability of Lactobacillus in human vagina[J]. Joural of Hygiene Research . 2006, 35(3):310-313. (in Chinese with English abstract)
[12] 倪学勤,曾东,邓宇,等. 玉米、豆粕和麦麸抽提物对4株猪源乳酸杆菌耐酸能力的影响[J]. 动物生产,2009, 45(13):46-50.
NI X Q, ZENG D, DENG Y et al. Effect of corn, soybean meal and wheat bran extracts on viability of probiotic Lactobacilli under acidic conditions[J]. Animal Production , 2009, 45(13):46-50. (in Chinese with English abstract)
[13] REID G, CHARBONNEAU D, ERB J, et al. Oral use of Lactobacillus rhamnosus GR-1 and L. fermentum RC-14 significantly alters vaginal flora: randomized, placebo-controlled trial in 64 healthy women[J]. FEMS Immunology and Medcine Microbiology , 2003, 35(2):131-134.
[14] MILLETTE M, LUQUET F M, LACROIX M. In vitro growth control of selected pathogens by Lactobacillus acidophilus and Lactobacillus casei fermented milk[J]. Letters in Applied Microbiology , 2007, 44(3):314-319.
[15] SILVA M, JACOBUS N V, DENEKE C, et al. Antimicrobial substance from a human Lactobacillus strain[J]. Antimicrobial Agents and Chemotherapy , 1987, 31(8):1231-1233.
[16] LIN C K, TSAI H C, LIN P P, et al. Lactobacillus acidophilus LAP5 able to inhibit the Salmonella choleraesuis invasion to the human Caco-2 epithelial cell[J]. Anaerobe , 2008, 14(5):251-255.
[17] 周勃. 乳酸菌耐热性的研究[J]. 饲料与畜牧,2010(增刊):47-48
ZHOU B. Lactic acid bacteria research of heat resistance[J]. Feed and Animal Husbandry .2010, (Suppl.):47-48. (in Chinese)
[18] 王旭明, 汪群慧, 姜华. 耐热乳杆菌的分离及在食物垃圾乳酸发酵中的应用[J]. 环境工程学报,2008, 2(8):1098-1102.
WANG X M,WANG Q H, JIANG H. Isolation of a thermotolerant Lactobacillus sp.and its application on production of lactic acid from food waste[J]. Chinese Journal of Environmental Engineering , 2008, 2(8):1098-1102. (in Chinese with English abstract)
[19] 张娜, 郭元昕, TADAYUKI IMANAKA, 等. 一株乳酸菌的鉴定、生物学特性与发酵培养基优化研究[J]. 食品工业,2012 (12):136-140.
ZHANG N, GUO Y X, TADAYUKI I, et al. Indentification of a strain of lactic acid bacteria and study on its biological properties and optimization of fermentation medium[J]. The Food Industry , 2012 (12):136-140. (in Chinese with English abstract)
[20] SERVIN A L. Antagonistic activities of Lactobacilli and bifidobacteria against microbial pathogens[J]. FEMS Microbiology Reviews . 2004, 28(4):405-440.
[21] VALERIO F, LAVERMICOCCA P, PASCALE M, et al. Production of phenyllactic acid by lactic acid bacteria: an approach to the selection of strains contributing to food quality and preservation[J]. FEMS Microbiology Letter . 2004, 233(2):289-295.
[22] ANNUK H, SHCHEPETOVA J, KULLISAAR T, et al. Characterization of intestinal lactobacilli as putative probiotic candidates[J]. Journal of Applied Microbiology , 2003, 94(3):403-412.
[23] HÜTT P, SHCHEPETOVA J, LÕIVUKENE K, et al. Antagonistic activity of probiotic lactobacilli and bifidobacteria against entero-and uropathogens[J]. Journal of Applied Microbiology , 2006, 100(6):1324-1332.
[24] JUÁREZ TOMÁS M S, OCAŇA V S, WIESE B, et al. Growth and lactic acid production by vaginal Lactobacillus acidophilus CRL 1259, and inhibition of uropathogenic Escherichia coli [J]. Journal of Medical Microbiology . 2003, 52(Pt 12):1117-1124.
[25] COOK S I, SELLIN J H. Review article: short chain fatty acids in health and disease[J]. Alimentary Pharmacology & Therapeutics , 1998, 12(6):499-507.
[26] 乔家运. 乳酸杆菌对断奶仔猪生长代谢及免疫机能影响的研究 [D], 郑州:河南农业大学,2005.
QIAO J Y. Study on effect of Lactobacillus on growth metabolism and the immunity of weaned piglets [D]. Zhengzhou: Henan Agricultural University, 2005. (in Chinese with English abstract)
[27] 熊鹿言, 唐海丰, 余倩. 正常人肠道不同乳酸杆菌菌种耐药性的研究[J]. 海南医学,2011, 22(23):65-68.
XIONG L Y, TANG H F, YU Q. Drug resistance of different Lactobacillus genus in healthy human gut.[J]. Hainan Medical Journal . 2011, 22(23):65-68. (in Chinese with English abstract)
[28] 虞泽鹏, 谢启轮, 唐举, 等. 益生素对断乳仔猪生产性能的影响[J]. 动物科学与动物医学,2002, 19(3):49-50.
YU Z P, XIE Q L, TANG J, et al. Effect of EM on the performance of weaned piglets[J]. Animal Science & Veterinary Medicine. 2002, 19(3):49-50. (in Chinese with English abstract)
[29] 朱珊珊, 崔雯, 任晓明. 乳酸杆菌黏附作用的研究进展[J]. 中国畜牧兽医,2009, 36(7):219-220.
ZHU S S, CUI W, REN X M. The adhesion effect of Lactobacillus of research progress[J]. China Animal Husbandry & Veterinary Medicine , 2009, 36(7):219-220. (in Chinese with English abstract)

大熊猫阴道源乳酸杆菌生物学特性研究

Study on biological characteristics of Lactobacillus from giant panda vagina

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