十三个柑橘品种叶片光合特性研究

doi:10.3969/j.issn.1004-1524.2017.06.09

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (6): 917-925.DOI: 10.3969/j.issn.1004-1524.2017.06.09

十三个柑橘品种叶片光合特性研究

邱霞¹, 叶霜¹, 熊博¹, 廖玲¹, 孙国超², 荣毅¹, 罗近予¹, 代琳¹, 汪志辉^1,2,*

1.四川农业大学园艺学院,四川成都 611130;
2.四川农业大学果蔬研究所,四川成都 611130

收稿日期:2017-02-07 出版日期:2017-06-20 发布日期:2017-09-07
通讯作者: 汪志辉,E-mail: wangzhihui_siau@126.com
作者简介:邱霞(1993—),女,四川成都人,硕士研究生,从事现代果树栽培与生理研究。E-mail: qiuxia1526@hotmail.com
基金资助:
四川省教育厅项目(14CZ0002); 四川省科技厅项目(2016NZ0056)

Photosynthetic characteristics of 13 citrus cultivars

QIU Xia¹, YE Shuang¹, XIONG Bo¹, LIAO Ling¹, SUN Guochao², RONG Yi¹, LUO Jinyu¹, DAI Lin¹, WANG Zhihui^1,2,*

1. College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China;
2. Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, China

Received:2017-02-07 Online:2017-06-20 Published:2017-09-07

摘要/Abstract

摘要： 为研究不同柑橘品种光合特性,试验以6类13个柑橘品种为材料,对其瞬时光合作用参数、光响应特性、CO₂响应特性和光合关键酶活性进行了测定与分析。结果表明,13个柑橘品种光合生理参数和光合酶活性存在显著差异,其光补偿点和光饱和点分别为11.99~60.00和523.88~1 155.73 μmol·m^-2·s^-1, CO₂补偿点和CO₂饱和点分别为20.01~72.02和1 364.44~1 811.29 μmol·mol^-1,Rubisco、RCA和PEPC活性分别为3.74~5.34、10.97~23.83和5.06~9.39 μ IU。综合分析不同柑橘光合参数和光合酶活性得知,合江佛手、黄果柑、巴西酸橙光合能力较强,大雅柑、晚红血橙和尤力克柠檬次之,爱媛38号和红肉琯溪蜜柚相对较低。在柑橘中检测到较高活性的C₄途径关键酶PEPC,其与Rubisco比值为0.95~2.26,说明在柑橘中可能存在微量的C₄途径。

关键词: 柑橘, 光合作用, 光响应, CO₂响应, 光合酶

Abstract: In order to study the photosynthetic characteristics of different citrus cultivars, 13 citrus varieties of 6 categories were used as test materials, and the instantaneous photosynthesis parameters, response characteristics to light, response characteristics to CO₂ and photosynthetic key enzyme activities were determined and analyzed. The results showed that there were significant differences in the photosynthetic parameters and photosynthetic enzyme activities among the 13 citrus cultivars. The light compensation point and the light saturation point were between 11.99-60.00 and 523.88-1 155.73μmol·m^-2·s^-1, respectively; the CO₂ compensation point and the CO₂ saturation point were between 20.01-72.02 and 1 364.44-1 811.29 μmol·mol^-1, respectively; the Rubisco, RCA and PEPC activity were respectively between 3.74-5.34, 10.97-23.83 and 5.06-9.39 μ IU, respectively. Comprehensive analysis of different citrus photosynthetic physiological parameters and photosynthetic enzyme activity showed that the photosynthetic capacity of Hejiang Bergamot, Huangguogan and Brazil Sour orange was strong; the photosynthetic capacity of Dayagan, Wanhong blood orange and Eureka lemon were the second; the photosynthetic capacity of Ehime Kashi.38 and Hongrou Guanxi pummelo were relatively worst. Higher activity of PEPC was detected in citrus, which is a key enzyme in the C₄ photosynthetic pathway, and the ratio of PEPC to Rubisco was between 0.95-2.26, indicating that there may be a weak C₄ photosynthetic pathway in citrus.

Key words: citrus, photosynthesis, light response, CO₂ response, photosynthetic enzymes

中图分类号:

S666

邱霞, 叶霜, 熊博, 廖玲, 孙国超, 荣毅, 罗近予, 代琳, 汪志辉. 十三个柑橘品种叶片光合特性研究[J]. 浙江农业学报, 2017, 29(6): 917-925.

QIU Xia, YE Shuang, XIONG Bo, LIAO Ling, SUN Guochao, RONG Yi, LUO Jinyu, DAI Lin, WANG Zhihui. Photosynthetic characteristics of 13 citrus cultivars[J]. , 2017, 29(6): 917-925.

参考文献

[1] 伍佳文,祁春节. 中国柑橘鲜果出口市场细分研究[J]. 湖南农业科学, 2014(16): 59-61.
WU J W, QI C J. Export market segmentation of China��s citrus fruit [J]. Hunan Agricultural Sciences , 2014 (16):59-61. (in Chinese with English abstract)
[2] 蔡楚雄,邓雄,曹洪麟,等. 6个芒果品种的叶绿素荧光日变化研究初报[J]. 广东农业科学,2003(2): 17-19.
CAI C X, DENG X, CAO H L, et al. Study on day-changing of chlorophyll fluorescence of 6 mango varieties [J]. Guangdong Agricultural Sciences , 2003 (2):17-19. (in Chinese with English abstract)
[3] 简水仙,李松伟,邓烈,等. 影响柑桔光合作用的内外因素研究综述[J]. 中国南方果树, 2011(4): 32-36.
JIAN S X, LI S W, DENG L, et al. A review of studies on internal and external factors affecting photosynthesis of citrus[J]. South China Fruits , 2011(4): 32-36. (in Chinese)
[4] 刘国琴,樊卫国,何嵩涛,等. 16个柑桔品种的光合特性[J]. 种子, 2003(5): 13-15.
LIU G Q, FAN W G, HE S T, et al. Study on photosynthetic characteristics of sixteen citrus cultivars[J]. Seed , 2003(5): 13-15. (in Chinese with English abstract)
[5] 宋勤飞,欧阳斌. 不同柑橘品种光合生理生态特性的日变化[J]. 贵州农业科学, 2009, 37(9): 178-181.
SONG Q F, OUYANG B. Daily variation of photosynthetic physio-ecological characteristics of different citrus cultivars[J]. Guizhou Agricultural Sciences , 2009, 37(9): 178-181. (in Chinese with English abstract)
[6] 黄永敬,唐小浪,马培恰,等. 几个柑橘品种叶片光合特性比较研究[J]. 中国农学通报, 2008, 24(12): 132-135.
HUANF Y J, TANG X L, MA P Q, et al. Comparison of photosynthetic characteristics among several citrus cultivars[J]. Chinese Agricultural Science Bulletin , 2008, 24(12):132-135. (in Chinese with English abstract)
[7] 叶子飘. 光合作用对光和CO 2 响应模型的研究进展[J]. 植物生态学报, 2010,34(6): 727-740.
YE Z P. A review on modeling of responses of photosynthesis to light and CO 2 [J]. Acta Phytoecologica Sinica , 2010, 34(6): 727-740. (in Chinese with English abstract)
[8] 周洪华,陈亚宁,李卫红,等. 干旱区胡杨光合作用对高温和CO 2 浓度的响应[J]. 生态学报, 2009,29(6): 2797-2810.
ZHOU H H, CHEN Y N, LI W H, et al. Photosynthesis of Populus euphratica olive and its response to CO 2 concentration and high temperature in arid environment[J]. Acta Ecologica Sinica , 2009, 29(6): 2797-2810. (in Chinese with English abstract)
[9] 廖玲,曹淑燕,荣毅,等. 不同砧木对黄果柑光合特性、光合作用关键酶及其基因表达的影响[J]. 浙江农业学报, 2016,28(5): 769-775.
LIAO L, CAO S Y, RONG Y, et al. Effects of different rootstocks on photosynthetic characteristics, activities and gene expres-sion of key enzymes of photosynthesis in Huangguogan[J]. Acta Agriculturae Zhejiangensis , 2016, 28(5): 769-775. (in Chinese with English abstract)
[10] 翁晓燕,毛伟华. 水稻叶片生育过程中Rubisco活化酶及其与Rubisco和光合速率的关系[J]. 浙江农业学报, 2000,12(3): 5-9.
WENG X Y, MAO W H. The relationship of Rubisco activase to Rubisco and photosynthetic rate during development of rice leaf[J]. Acta Agriculturae Zhejiangensis , 2000, 12(3):5-9. (in Chinese with English abstract)
[11] WHITNEY S M, ANDREWS T J. Plastome-encoded bacterial ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) supports photosynthesis and growth in tobacco[J]. Proceedings of the National Academy of Sciences of the United States of America , 2001, 98(25): 14738-14743.
[12] JENKINS C L, FURBANK R T, HATCH M D. Mechanism of C 4 photosynthesis a model describing the inorganic carbon pool in bundle sheath cells[J]. Plant Physiology , 1989,91(4): 1372-1381.
[13] KELLY G J, LATZKO E. Chloroplast phosphofructokinase: I. proof of phosphofructokinase activity in chloroplasts[J]. Plant Physiology , 1977, 60(2): 290-294.
[14] 汪小福,刘仁虎,陈笑芸,等. 甘蓝型油菜磷酸烯醇式丙酮酸羧化酶的研究分析[J]. 浙江农业学报, 2010,22(3): 276-280.
WANG X F, LIU R H, CHEN X Y. et al. Analysis of Brassica napus phosphoenolpyruate carboxylase (PEPC)[J]. Acta Agriculturae Zhejiangensis , 2010, 22(3): 276-280. (in Chinese with English abstract)
[15] HIRATSUKA S, SUZUKI M, NISHIMURA H, et al. Fruit photosynthesis in Satsuma mandarin [J]. Plant Science , 2015, 241: 65-69.
[16] 叶子飘,杨小龙,康华靖. C 3 和C 4 植物光能利用效率和水分利用效率的比较研究[J]. 浙江农业学报, 2016, 28(11): 1867-1873.
YE Z P, YANG X L, KANG H J. Comparison of light-use and water-use efficiency for C 3 and C 4 species[J]. Acta Agriculturae Zhejiangensis , 2016, 28(11): 1867-1873. (in Chinese with English abstract)
[17] 吴文超,曲延英,高文伟,等. 不同棉花品种对盐、旱胁迫的光合响应及抗逆性评价[J]. 新疆农业科学, 2016,53(9): 1569-1579.
WU W C, QU Y Y, GAO W W, et al. Evaluation of cotton stress resistance based on the responses of the photosynthetic indexes to salt and drought stress [J]. Xinjiang Agricultural Sciences , 2016, 53(9): 1569-1579. (in Chinese with English abstract)
[18] 胡利明,夏仁学,周开兵,等. 不同砧木对温州蜜柑光合特性的影响[J]. 园艺学报, 2006, 33(5): 937-941.
HU L M, XIA R X, ZHOU K B, et al. Effects of different rootstocks on the photosynthesis of Satsuma mandarin [J]. Acta Horticulturae Sinica , 2006,33(5): 937-941. (in Chinese with English abstract)
[19] 曾光辉,郭延平,王法格,等. 冬、春季节柑桔叶片光合机构运转的研究[J]. 浙江大学学报(农业与生命科学版), 2006,32(4): 410-414.
ZENG G H, GUO Y P, WANG F G, et al. Operation of photosynthetic apparatus of citrus leaves in winter and spring[J]. Journal of Zhejiang University ( Agriculture & Life Sciences ), 2006,32(4): 410-414. (in Chinese with English abstract)
[20] 郭卫东,郑建树,张真真,等. 短暂低温对佛手光合生理的影响[J]. 生态学报, 2009, 29(5): 2286-2293.
GUO W D, ZHENG J S, ZHANG Z Z, et al. Effects of short term chilling stress on the photosynthetic physiology of fingered citrons ( Citrus medica var. sarcodactylis Swingle) [J]. Acta Ecologica Sinica , 2009, 29(5): 2286-2293. (in Chinese with English abstract)
[21] 王海珍,韩路,徐雅丽,等. 胡杨异形叶光合作用对光强与CO 2 浓度的响应[J]. 植物生态学报, 2014,38(10): 1099-1109.
WANG H Z, HAN L, XU Y L, et al. Photosynthetic responses of the heteromorphic leaves in Populus euphratica to light intensity and CO 2 concentration[J]. Acta Phytoecologica Sinica , 2014, 38(10): 1099-1109. (in Chinese with English abstract)
[22] 王雁,马武昌. 扶芳藤、紫藤等7种藤本植物光能利用特性及耐荫性比较研究[J]. 林业科学研究, 2004,17(3): 305-309.
WANG Y, MA W C. Comparative studies on light utilization characteristics and shade tolerance of 7 climbing shrub species[J]. Forest Research , 2004,17(3):305-309. (in Chinese with English abstract)
[23] IDSO S B, KIMBALL B A. Effects of atmospheric CO 2 enrichment on photosynthesis, respiration, and growth of sour orange trees[J]. Plant Physiology , 1992, 99(1): 341-343.
[24] ALLEN L H, VU J C V. Carbon dioxide and high temperature effects on growth of young orange trees in a humid, subtropical environment[J]. Agricultural and Forest Meteorology , 2009, 149(5): 820-830.
[25] 田秀英. RuBP羧化酶加氧酶的研究进展[J]. 重庆师专学报,2000,19(3): 77-79.
TIAN X Y. The recent research in ribulose 1,5-bisphosphate carboxylase/oxygenase[J]. Journal of Chongqing Teachers College , 2000,19(3): 77-79. (in Chinese with English abstract)
[26] JORDAN D B, OGREN W L. The CO 2 /O 2 specificity of ribulose 1,5-bisphosphate carboxylase/oxygenase: Dependence on ribulosebisphosphate concentration, pH and temperature[J]. Planta , 1984, 161(4): 308-313.
[27] 韩春丽. 弱光下纽荷尔脐橙光合生理特性研究[D]. 武汉:华中农业大学, 2008.
HAN C L. Studies on the photosynthetic physiological characteristics of Citrus sinensis cv. Newhall leaves under low light stress[D]. Wuhan: Huazhong Agricultural University, 2008. (in Chinese with English abstract)
[28] CHRISTIN P A, ARAKAKI M, OSBORNE C P, et al. Shared origins of a key enzyme during the evolution of C 4 and CAM metabolism[J]. Journal of Experimental Botany , 2014, 65(13): 3609-3621.
[29] KU M S, AGARIE S, NOMURA M, et al. High-level expression of maize phosphoenolpyruvate carboxylase in transgenic rice plants[J]. Nature Biotechnol ogy, 1999, 17(1): 76-80.
[30] 李合生. 现代植物生理学 [M]. 3版. 北京:高等教育出版社, 2012: 160-169.
[31] BOWES G. Chapter 5 Single-cell C 4 photosynthesis in aquatic plants[M]// RAGHAVENDRA A S, SAGE R F. C4 photosynthesis and related CO2 concentrating mechanisms. Netherlands: Springer, 2011: 63-80.

十三个柑橘品种叶片光合特性研究

Photosynthetic characteristics of 13 citrus cultivars

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