西南部分地区Ⅰ群禽腺病毒的分子流行病学调查及致病性研究

doi:10.3969/j.issn.1004-1524.2017.11.06

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (11): 1809-1818.DOI: 10.3969/j.issn.1004-1524.2017.11.06

西南部分地区Ⅰ群禽腺病毒的分子流行病学调查及致病性研究

姚克昌, 刘月月, 游国进, 李淑芸, 夏静, 何肖, 李雯雯, 杜莉静, 韩新锋, 黄勇^*

四川农业大学动物医学院,四川成都 611130

收稿日期:2017-05-12 出版日期:2017-11-20 发布日期:2017-12-05
通讯作者: 黄勇,E-mail:hyong601@163.com
作者简介:姚克昌(1989—),男,河南商丘人,硕士研究生,主要从事家禽传染病的研究。E-mail:qingbuykc@163.com

Pathogenicity and epidemiological investigation of outbreaks of fowl adenovirus subpopulation Ⅰ infection in chickens in parts of southwestern China

YAO Kechang, LIU Yueyue, YOU Guojin, LI Shuyun, XIA Jing, HE Xiao, LI Wenwen, DU Lijing, HAN Xinfeng, HUANG Yong^*

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2017-05-12 Online:2017-11-20 Published:2017-12-05

摘要/Abstract

摘要： 2015—2016年,西南地区多个养鸡场发生疑似禽腺病毒感染病例。实验从发病鸡场采集肝组织,利用鸡胚肾细胞(CEK)进行病毒分离,共分离出16株Ⅰ群禽腺病毒(FAdV)。通过对hexon基因L1环临近的保守序列进行PCR扩增和序列测定,发现16株FAdV分属4个血清型,其中13株为FAdV-4型,FAdV-8a,FAdV-8b和FAdV-2型各占1株。选取不同血清型的4个代表毒株CH/SCDY/1604(FAdV-4)、CH/GZXF/1512(FAdV-2)、CH/CQBS/1504 (FAdV-8a)、CH/CQBS/1512(FAdV-8b)进行鸡的致病性实验,结果显示,感染CH/SCDY/1604(FAdV-4)的死亡率达80%,CH/CQBS/1504(FAdV-8a)感染组死亡率为20%,而CH/GZXF/1512(FAdV-2)和CH/CQBS/1512(FAdV-8b)组的鸡只有发病没有死亡。FAdV-4是西南地区流行的主要血清型且其致病性极高,其致病性主要表现为心包积液综合征(HPS)。试验结果可为西南地区Ⅰ群禽腺病毒感染的防治提供参考。

关键词: Ⅰ, 群禽腺病毒, 心包积水综合征, 流行病学调查, 致病性

Abstract: In 2015-2016, suspected fowl adenoviruses infections were observed in many chicken farms in southwestern China. The liver samples were collected from diseased chickens and inoculated into chicken embryo kidney cells (CEK) cultures for virus isolation, and 16 fowl adenoviruses (FAdV) strains were isolated. After PCR amplificating, sequencing and comparing the conserved sequences located in the pedestal regions adjacent to the L1 loop of the hexon genes of FAdV, all of the 16 stains could be grouped into four serotypes including FAdV-4(13/16), FAdV-8a (1/16), FAdV-8 (1/16) and FAdV-2 (1/16). Then, the delegate strains from those four different serotypes were selected to infect chickens to observe its pathogenicity to chickens, the results showed that the mortality of chickens infected with CH/SCDY/1604(FAdV-4) and CH/CQBS/1504 (FAdV-8a) was 80% and 20%, respectively, while no death of chicken was observed in chickens infected by CH/GZXF/1512(FAdV-2)and CH/CQBS/1512(FAdV-8b). It was concluded that the FAdV-4 was the predominant serotype and was highly pathogenic to chickens, and hydropericardium syndrome (HPS) was the main clinical manifestation. This results could provide reference for the epidemiological investigation and prevention of FAdVⅠinfection in southwestern China.

Key words: fowl adenovirus, hydropericardium syndrome, epidemiological investigation, pathogenicity

中图分类号:

S852.65⁺7

姚克昌, 刘月月, 游国进, 李淑芸, 夏静, 何肖, 李雯雯, 杜莉静, 韩新锋, 黄勇. 西南部分地区Ⅰ群禽腺病毒的分子流行病学调查及致病性研究[J]. 浙江农业学报, 2017, 29(11): 1809-1818.

YAO Kechang, LIU Yueyue, YOU Guojin, LI Shuyun, XIA Jing, HE Xiao, LI Wenwen, DU Lijing, HAN Xinfeng, HUANG Yong. Pathogenicity and epidemiological investigation of outbreaks of fowl adenovirus subpopulation Ⅰ infection in chickens in parts of southwestern China[J]. , 2017, 29(11): 1809-1818.

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西南部分地区Ⅰ群禽腺病毒的分子流行病学调查及致病性研究

Pathogenicity and epidemiological investigation of outbreaks of fowl adenovirus subpopulation Ⅰ infection in chickens in parts of southwestern China

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