青藏高原5种类型土壤细菌群落结构差异

doi:10.3969/j.issn.1004-1524.2017.11.15

浙江农业学报 ›› 2017, Vol. 29 ›› Issue (11): 1882-1889.DOI: 10.3969/j.issn.1004-1524.2017.11.15

青藏高原5种类型土壤细菌群落结构差异

王信^{1, 2}, 程亮^{2, 3, *}

1.青海大学农林科学院土壤肥料研究所,青海西宁 810016;
2.青海大学省部共建三江源生态与高原农牧业国家重点实验室,青海西宁 810016;
3.青海大学农林科学院植物保护研究所,青海西宁 810016

收稿日期:2017-05-05 出版日期:2017-11-20 发布日期:2017-12-05
通讯作者: 程亮,E-mail:liangcheng1979@163.com
作者简介:王信(1975—),女,河南商丘人,硕士,副研究员,主要从事微生物资源利用研究工作。E-mail:wangx710@163.com
基金资助:
青海省科技计划项目基础研究计划(2015-ZJ-705); 青海大学中青年科研基金项目(2015-QNY-2, 2014-QNY-7)

Soil bacterial community composition and diversity of five soil types in Qinghai-Tibetan Plateau

WANG Xin^{1, 2}, CHENG Liang^{2, 3, *}

1. Institute of Soil and Fertilizer, Qinghai Academy of Agriculture and Forestry Sciences, Qinghai University, Xi’ning 810016, China;
2. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xi’ning 810016, China;
3. Institute of Plant Protection, Qinghai Academy of Agriculture and Forestry Sciences, Qinghai University, Xi’ning 810016, China

Received:2017-05-05 Online:2017-11-20 Published:2017-12-05

摘要/Abstract

摘要： 于2015年8月采集青藏高原5种类型(栗钙土、山地草甸土、黑钙土、灰漠土和灰褐土)的15 cm土壤,采用Illumina高通量测序技术研究土壤细菌群落结构及多样性,并结合土壤因子对土壤细菌群落结构和多样性进行相关性分析。结果表明,土壤类型中有机碳和总氮含量的大小排序为栗钙土>山地草甸土>黑钙土>灰漠土>灰褐土,样地海拔与土壤养分的积累量没有相关性。土壤细菌群落相对丰度在5%以上的优势类群包括变形菌门、放线菌门、酸杆菌门、拟杆菌门、绿弯菌门和厚壁菌门6大类。栗钙土和山地草甸土土壤细菌α多样性(物种丰富度和系统发育多样性)显著(P<0.05)高于其他土壤类型;灰漠土细菌系统发育多样性显著(P<0.05)低于其他类型。灰褐土细菌物种丰富度最低。典范对应分析和Pearson相关性分析表明,土壤含水量、钾含量、有机碳和总氮含量是土壤细菌群落结构和α多样性的主要影响因子。

关键词: 土壤类型, 土壤细菌群落, 高通量测序

Abstract: In August 2015, soil bacterial diversity under five soil types (Castanozeras, mountain meadow soil, chernozems, gray desert soil, gray-cinnamon soil) was measured using Illumina high-throughput sequencing. It was shown that soil total organic carbon and total nitrogen varied in the order of castanozeras>mountain meadow soils>chernozems>gray desert soils>gray-cinnamon soils. There was no correlation between soil nutrient accumulation and the elevation of the sampling sites. The dominant phyla across all the soils were Proteobacteria, Actinobacteria, Acidobacteri, Bacteroidetes, Chloroflexi and Firmicutes. The bacteria diversity (species richness and phylogenetic diversity) of castanozeras and mountain meadow soils was significantly (P<0.05) higher than that of other soil types. Phylogenetic diversity in gray desert soil was significantly (P<0.05) lower than that of other soil types. The lowest phylotype richness was observed in gray-cinnamon soil. The results of the canonical correspondence and Pearson correlation analysis showed that soil moisture content, and contents of potassium, total organic carbon and total nitrogen were the main factors that affected the soil bacterial community composition and alpha diversity in these five soil types.

Key words: soil type, soil bacterial community, high-throughput sequencing

中图分类号:

S154.36

王信, 程亮. 青藏高原5种类型土壤细菌群落结构差异[J]. 浙江农业学报, 2017, 29(11): 1882-1889.

WANG Xin, CHENG Liang. Soil bacterial community composition and diversity of five soil types in Qinghai-Tibetan Plateau[J]. , 2017, 29(11): 1882-1889.

参考文献

[1] MOCALI S, BENEDETTI A. Exploring research frontiers in microbiology: the challenge of metagenomics in soil microbiology[J]. Research in Microbiology , 2010,161 (6):497-505.
[2] ARIAS M E, GONZALEZ-PEREZ J A, GONZALEZ-VILA F J, et al. Soil health-a new challenge for microbiologists and chemists[J]. International Microbiology , 2005, 8(1):13-21.
[3] TALBOT J M, BRUNS T D, TAYLOR J W, et al. Endemism and functional convergence across the North American soil mycobiome[J]. Proceedings of the National Academy of Sciences of the United States of America , 2014, 111(17):6341-6346.
[4] 李晨华,张彩霞,唐立松,等.长期施肥土壤微生物群落的剖面变化及其与土壤性质的关系[J].微生物学报,2014,54(3):319-329.
LI C H, ZHANG C X, TANG L S, et al. Effect of long-term fertilizing regime on soil microbial diversity and soil property[J]. Acta Microbiologica Sinica , 2014, 54(3):319-329. (in Chinese with English abstract)
[5] 郑燕,贾仲君.新一代高通量测序与稳定性同位素示踪DNA/RNA技术研究稻田红壤甲烷氧化的微生物过程[J].微生物学报,2013,53(2):173-184.
ZHENG Y, JIA Z J. Next generation sequencing and stable isotope probing of active microorganisms responsible for aerobic methane oxidation in red paddy soils[J]. Acta Microbiologica Sinica , 2013,53(2):173-184. (in Chinese with English abstract)
[6] 陆雅海, 傅声雷, 褚海燕,等. 全球变化背景下的土壤生物学研究进展[J]. 中国科学基金, 2015(1):19-24.
LU Y H, FU S L, CHU H Y, et al. Recent advances global change and soil biology[J]. Bulletin of National Natural Science Foundation of China , 2015 (1): 19-24. (in Chinese with English abstract)
[7] 岳君,刘光绣,章高森,等.念青唐古拉山扎当冰川退缩前沿土壤性质与可培养细菌多样性变化[J].冰川冻土,2010,32(6):1180-1185.
YUE J, LIU G X, ZHANG G S, et al. Changes in soil properties and culturable bacteria diversity in Zhadang Glacier Foreland[J]. Journal of Glaciology and Geocryology , 2010, 32(6):1180-1185.(in Chinese with English abstract)
[8] ZHANG G, MA X,NIU F,et a1.Diversity and distribution of alkaliphilic psychrotolerant bacteria in the Qinghai-Tibet Plateau permafrost region[J]. Extremophiles , 2007, 11(3):415-424.
[9] 王长庭,龙瑞军,王根绪,等.高寒草甸群落地表植被特征与土壤理化性状、土壤微生物之间的相关性研究[J].草业学报,2010,19(6):25-34.
WANG C T, LONG R J, WANG G C, et al. Relationship between plant communities, characters, soil physical and chemical properties,and soil microbiology in alpine meadows[J]. Acta Prataculturae Sinica , 2010,19(6):25-34. (in Chinese with English abstract)
[10] GUAN X Y, WANG J F, ZHAO H, et al. Soil bacterial communities shaped by geochemical factors and land use in a less-explored area, Tibetan Plateau[J]. BMC Genomics , 2013, 14(1):820.
[11] YUN J,JU Y,DENG Y,et al. Bacterial community structure in two permafrost wetlands on the Tibetan Plateau and Sanjiang Plain, China[J]. Microbial Ecology , 2014, 68(2): 360-369.
[12] 张甘霖,龚子同.土壤调查实验室分析方法[M].北京:科学出版社,2012:23-76.
[13] BROOKES P C, LANDMAN A, PRUDEN G, et al. Chloroform fumigation and the release of soil nitrogen: a rapid direct extraction method to measure microbial biomass nitrogen in soil[J]. Soil Biology & Biochemistry , 1985, 17(6):837-842.
[14] VANCE E D, BROOKES P C, JENKINSON D S. An extraction method for measuring soil microbial biomass C[J]. Soil Biology & Biochemistry , 1987, 19(6):703-707.
[15] GRANDY A S, STRICKLAND M S, LAUBER C L, et al. The influence of microbial communities, management, and soil texture on soil organic matter chemistry[J]. Geoderma , 2009, 150(3/4):278-286.
[16] MELO V S, DESJARDINS T, SILVA JR M L, et al. Consequences of forest conversion to pasture and fallow on soil microbial biomass and activity in the eastern Amazon[J]. Soil Use & Management , 2012, 28(4):530-535.
[17] 王启兰,曹广民,王长庭.高寒草甸不同植被土壤微生物数量及微生物生物量的特征[J].生态学杂志,2007,26(7): 1002-1008.
WANG Q L, CAO G M, WANG C T. Quantitative characters of soil microbes and microbial biomass under different vegetations in alpine meadow[J]. Chinese Journal of Ecology , 2007, 26(7): 1002-1008. (in Chinese with English abstract)
[18] WARDLE D A. A comparative assessment of factors which influence microbial biomass carbon and nitrogen levels in soil[J]. Biological Reviews , 1992, 67(3):321-358.
[19] LIU J J, SUI Y Y, YU Z H, et al. High throughput sequencing analysis of biogeographical distribution of bacterial communities in the black soils of northeast China[J]. Soil Biology & Biochemistry , 2014, 70 (2):113-122.
[20] SHEN C C, XIONG J B, ZHANG H Y, et al. Soil pH drives the spatial distribution of bacterial communities along elevation on Changbai Mountain[J]. Soil Biology & Biochemistry , 2013, 57:204-211.
[21] CHU H Y, SUN H B, TRIPATHI B M, et al. Bacterial community dissimilarity between the surface and subsurface soils equals horizontal differences over several kilometers in the western Tibetan Plateau[J]. Environmental Microbiology , 2016, 18(5):1523-1533.
[22] YUAN Y L, SI G C, WANG J, et al. Bacterial community in alpine grasslands along altitudinal gradient on the Tibetan Plateau[J]. FEMS Microbiology Ecology , 2014, 87(1):121-132.
[23] Zhang X F, Zhao L, Xu S J, et al. Soil moisture effect on bacterial and fungal community in Beilu River (Tibetan Plateau) permafrost soils with different vegetation types[J]. Journal of Applied Microbiology , 2013, 114(4):1054-1065.
[24] TAO J M, LIU X D, LIANG Y L, et al. Maize growth responses to soil microbes and soil properties after fertilization with different green manures[J]. Applied Microbiology and Biotechnology , 2017, 101(3):1289-1299.
[25] FIERER N, JACKSON R B. The diversity and biogeography of soil bacterial communities[J]. Proceedings of the National Academy of Sciences of the United States of America , 2006, 103(3):626-631.

青藏高原5种类型土壤细菌群落结构差异

Soil bacterial community composition and diversity of five soil types in Qinghai-Tibetan Plateau

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	徐民民, 黄莹, 李波, 徐艳, 张帅, 姚岭芸, 王政. 生物炭对小麦根际和根内微生物群落结构的影响[J]. 浙江农业学报, 2021, 33(3): 516-525.
[2]	陈乾丽, 汪汉成, 梁永进, 蔡刘体, 黄宇, 周浩, 李忠, 韩洁. 烤后健康烟叶和霉烂烟叶真菌群落结构分析[J]. 浙江农业学报, 2020, 32(6): 1019-1028.
[3]	杨移斌, 艾晓辉, 宋怿, 董靖, 胥宁, 姜兰. 黄颡鱼溶血性腹水病初探[J]. 浙江农业学报, 2019, 31(8): 1239-1248.
[4]	张博伟, 王海静, 宋茂勇, 谢慧君, 张建. 四溴双酚A对土壤无机氮转化的影响及其微生物学机理初探[J]. 浙江农业学报, 2019, 31(4): 639-645.
[5]	倪黎纲, 赵旭庭, 王宵燕, 宋成义, 吴信生, 甘源. 基于高通量测序分析肺炎支原体感染姜曲海猪肺组织miRNA表达谱[J]. 浙江农业学报, 2019, 31(12): 1979-1986.
[6]	徐晓锋, 郭成. 淀粉诱导奶牛乳脂下降后奶牛瘤胃细菌菌群变化[J]. 浙江农业学报, 2019, 31(10): 1591-1598.
[7]	张爱菊, 郝雅宾, 郭爱环, 刘金殿, 练青平, 周志明. 基于高通量测序技术的鱼类环境DNA研究中通用引物的筛选验证[J]. 浙江农业学报, 2019, 31(10): 1615-1623.
[8]	桂国弘, 杨华, 朱江群, 朱建芬, 肖英平, 徐娥. 冷鲜鸡冷藏保存过程中菌群结构变化分析[J]. 浙江农业学报, 2019, 31(1): 47-55.
[9]	刘宗楠, 王新, 吴逸飞, 姚晓红, 孙宏, 沈琦, 李维琳, 汤江武. 鸢尾联合固定化菌剂净化河道水体的微生态过程[J]. 浙江农业学报, 2019, 31(1): 121-129.
[10]	王佩佩, 杨华, 戴贤君, 桂国弘, 肖英平. 家禽屠宰场环境和屠宰器械表面微生物菌群结构和耐药基因分析[J]. 浙江农业学报, 2018, 30(7): 1249-1258.
[11]	肖英平, 杨彩梅, 代兵, 李开锋, 陈镜刚, 杨华. 基于高通量测序的丁酸梭菌对肉鸡盲肠菌群结构的影响[J]. 浙江农业学报, 2017, 29(3): 373-379.
[12]	席刚俊, 李警保, 史俊, 韩正敏. 白芨内生真菌的多样性[J]. 浙江农业学报, 2017, 29(12): 2077-2083.
[13]	陈芸, 刘旗, 邓俊良, 杨颜铱, 高爽, 陈憧, 姚淑华. 复合抗菌肽对山羊瘤胃菌群结构的影响[J]. 浙江农业学报, 2017, 29(11): 1800-1808.
[14]	王强, 姜丽娜, 潘建清, 李建强, 符建荣, 马军伟, 叶静, 俞巧钢, 孙万春, 邹平, 林辉. 长江下游单季稻一次性施肥产量效应及影响因子研究[J]. 浙江农业学报, 2017, 29(11): 1875-1881.
[15]	马婧1，成铁龙1，2，*，孙灿岳3，邓楠1，史胜青1，江泽平1. 草麻黄高通量转录组分析及黄酮类代谢途径相关基因的鉴定[J]. 浙江农业学报, 2016, 28(4): 609-.