转基因水稻中转人乳铁蛋白的抗氧化及免疫作用

doi:10.3969/j.issn.1004-1524.2018.01.01

浙江农业学报 ›› 2018, Vol. 30 ›› Issue (1): 1-6.DOI: 10.3969/j.issn.1004-1524.2018.01.01

转基因水稻中转人乳铁蛋白的抗氧化及免疫作用

秦毅¹, 吕国英², 张作法^{2, *}

1.浙江大学技术转移中心,浙江杭州 310058;
2.浙江省农业科学院园艺研究所,浙江杭州 310021

收稿日期:2017-06-29 出版日期:2018-01-20 发布日期:2018-02-09
通讯作者: 张作法,E-mail: zzf2050@163.com
作者简介:秦毅(1981—),女,河南新乡人,博士,助理研究员,主要从事转基因农作物领域的研发和成果转化。E-mail: yqinzju@zju.edu.cn
基金资助:
浙江大学基本科研业务费专项资金项目(2015XZZX002-03)

Studies on immunomodulatory and antioxidant activities in vitro of recombinant human lactoferrin from transgenic rice

QIN Yi¹, LYU Guoying², ZHANG Zuofa^{2, *}

1. Technology Transfer Center, Zhejiang University, Hangzhou 310058, China;
2. Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2017-06-29 Online:2018-01-20 Published:2018-02-09

摘要/Abstract

摘要： 以转人乳铁蛋白为研究对象,研究其抗氧化和免疫活性。采用DPPH法、ABTS法和羟基自由基清除能力的方法来研究转人乳铁蛋白(rhLF)的抗氧化功能,通过小鼠巨噬细胞RAW264.7的细胞增殖和吞噬活性的检测来测定转人乳铁蛋白的免疫活性。结果表明,转人乳铁蛋白对DPPH、ABTS和羟基自由基具有一定的清除能力,并能促进免疫细胞的增殖和增强免疫细胞的吞噬活性。转人乳铁蛋白具有较好的免疫活性和抗氧化活性,是开发相关保健食品的重要资源。

关键词: 转人乳铁蛋白, 抗氧化, 免疫作用

Abstract: In the present study, the immunomodulatory and antioxidant activities of the recombinant human lactoferrin (rhLF) were studied. The antioxidant activities of rhLF were investigated by DPPH, ABTS and hydroxyl radical scavenging activities and the immunomodulatory activities were determined by the detection of cell proliferation and phagocytic activity of RAW264.7. It turns out that rhLF exhibited scavenging activity against DPPH, ABTS and OH· radical as well as immunomodulatory activities. rhLF possessed excellent antioxidant and immunomodulatory activities, and was the important resource of the exploitation of health-care food.

Key words: recombinant human lactoferrin, antioxidant activity, immunomodulatory activity

中图分类号:

S511

秦毅, 吕国英, 张作法. 转基因水稻中转人乳铁蛋白的抗氧化及免疫作用[J]. 浙江农业学报, 2018, 30(1): 1-6.

QIN Yi, LYU Guoying, ZHANG Zuofa. Studies on immunomodulatory and antioxidant activities in vitro of recombinant human lactoferrin from transgenic rice[J]. , 2018, 30(1): 1-6.

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转基因水稻中转人乳铁蛋白的抗氧化及免疫作用

Studies on immunomodulatory and antioxidant activities in vitro of recombinant human lactoferrin from transgenic rice

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