紫花苜蓿MsOXO基因的克隆及表达分析

doi:10.3969/j.issn.1004-1524.2019.01.02

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (1): 11-19.DOI: 10.3969/j.issn.1004-1524.2019.01.02

紫花苜蓿MsOXO基因的克隆及表达分析

许青松^1,2, 赵佳^1,2, 魏运民^1,2, 韩蓉蓉^1,2, 刘卢生^1,2, 蒋曹德^1,2, 玉永雄^1,2,*

1.西南大学动物科技学院,重庆 400715;
2.重庆市高校草食动物工程中心,重庆 400715

收稿日期:2018-08-03 出版日期:2019-01-25 发布日期:2019-04-09
通讯作者: 玉永雄,E-mail: yuyongxiong8@126.com
作者简介:许青松(1994—),女,山东莱芜人,硕士研究生,主要从事牧草遗传育种研究。E-mail: xuqingsong2018@163.com
基金资助:
国家973计划(2014CB138700); 中央高校基本科研业务费专项(XDKJ2017C010)

Cloning and expression analysis of oxalate oxidase gene MsOXO from Medicago sativa

XU Qingsong^1,2, ZHAO Jia^1,2, WEI Yunmin^1,2, HAN Rongrong^1,2, LIU Lusheng^1,2, JIANG Caode^1,2, YU Yong-xiong^1,2,*

1. College of Animal Science and Technology, Southwest University, Chongqing 400715, China;
2. College Herbivore Engineering Center of Chongqing, Chongqing 400715, China

Received:2018-08-03 Online:2019-01-25 Published:2019-04-09

摘要/Abstract

摘要： 草酸氧化酶(OXO)在包括核盘菌在内的多种病原体的防御和植物改良中有重大作用。以紫花苜蓿为材料,克隆得到紫花苜蓿草酸氧化酶基因MsOXO。利用烟草瞬时表达进行亚细胞定位,结果显示,MsOXO定位在细胞壁上。qRT-PCR结果显示,MsOXO在紫花苜蓿的根、茎、叶中均有表达,但在根茎中表达量较低,这可能是根颈和茎基部更易被病菌侵染的原因。外源草酸50 μmol·L^-1处理根或100 μmol·L^-1处理茎及外源水杨酸100 μmol·L^-1处理根均可以诱导MsOXO基因的上调表达。

关键词: 紫花苜蓿, 草酸氧化酶, 基因克隆, 亚细胞定位

Abstract: Oxalate oxidase plays an important role in defence of various pathogens such as Sclerotinia and can be used for plant improvement. To explore the role of oxalate oxidase in the defense of Sclerotinia sclerotiorum, alfalfa was used for material in this study, an oxalate oxidase gene, MsOXO, was successfully cloned. Subcellular localization of MsOXO was performed using the transient expression of tobacco, and the result showed that MsOXO was located in cell wall. The results of qRT-PCR showed that MsOXO was expressed in roots, stems and leaves of alfalfa, but was lower in roots and stems, which might be the reason why the root neck and stem base were more susceptible to infection by the pathogen. The up-regulated expression of MsOXO gene was induced by exogenous oxalic acid 50 μmol·L^-1 treated root or 100 μmol·L^-1 treated stem and exogenous salicylic acid 100 μmol·L^-1 treated root.

Key words: Medicago sativa, oxalate oxidase, gene cloning, subcellular localization

中图分类号:

S963.22⁺3.3

许青松, 赵佳, 魏运民, 韩蓉蓉, 刘卢生, 蒋曹德, 玉永雄. 紫花苜蓿MsOXO基因的克隆及表达分析[J]. 浙江农业学报, 2019, 31(1): 11-19.

XU Qingsong, ZHAO Jia, WEI Yunmin, HAN Rongrong, LIU Lusheng, JIANG Caode, YU Yong-xiong. Cloning and expression analysis of oxalate oxidase gene MsOXO from Medicago sativa[J]. , 2019, 31(1): 11-19.

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紫花苜蓿MsOXO基因的克隆及表达分析

Cloning and expression analysis of oxalate oxidase gene MsOXO from Medicago sativa

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