浙茄类型茄子品种DNA指纹图谱构建

doi:10.3969/j.issn.1004-1524.2019.11.12

浙江农业学报 ›› 2019, Vol. 31 ›› Issue (11): 1863-1870.DOI: 10.3969/j.issn.1004-1524.2019.11.12

浙茄类型茄子品种DNA指纹图谱构建

魏庆镇, 王五宏, 胡天华, 胡海娇, 汪精磊, 包崇来^*

浙江省农业科学研究院蔬菜研究所,浙江杭州 310021

收稿日期:2019-07-09 出版日期:2019-11-25 发布日期:2019-12-04
作者简介:魏庆镇(1988—),男,山东泰安人,博士,助理研究员,主要从事蔬菜遗传育种与分子生物学研究。E-mail: wqz0619@163.com
通讯作者: *,包崇来,E-mail: baocl@mail.zaas.ac.cn
基金资助:
浙江省育种专项-茄子新品种选育和育种技术研究(2016C02051-2-1)

Construction of DNA fingerprinting of Zhejiang eggplant varieties

WEI Qingzhen, WANG Wuhong, HU Tianhua, HU Haijiao, WANG Jinglei, BAO Chonglai^*

Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2019-07-09 Online:2019-11-25 Published:2019-12-04

摘要/Abstract

摘要： 为了高效快速地区分浙茄类型茄子品种,并为鉴定浙茄品种的特异性和真实性提供依据,利用46份茄子材料,对48个单核苷酸多态性(SNP)标记进行核心SNP标记筛选,并利用筛选到的核心SNP标记,构建了6个浙茄类型茄子品种的SNP指纹图谱。结果表明:48个SNP标记均可转化为KASP标记,对46份茄子材料进行SNP标记的KASP分型,根据分型结果和多态性信息含量,34个SNP标记可作为核心标记,利用这些核心标记构建了6个浙茄类型茄子品种的指纹图谱;通过指纹图谱可以快速高效区分6个浙茄类型茄子品种,为茄子品种鉴定和知识产权的保护提供了理论依据。

关键词: 茄子, 主要品种, SNP标记, 指纹图谱

Abstract: In order to effectively and quickly identify and distinguish eggplant varieties of Zhejiang eggplant, and to provide the basis for the identification of the specificity and authenticity of Zhejiang eggplant varieties, SNP markers developed from eggplant genome data were screened, and SNP fingerprints were constructed for 6 eggplant varieties of Zhejiang eggplant type. Results showed that 48 SNPS were converted into KASP markers, and then used to KASP genotyping for 46 eggplant materials. According to the genotyping results and polymorphism information, 34 SNP markers could be used as core markers, which were used to construct a fingerprint map of the 6 Zhejiang eggplant varieties. The fingerprint map constructed herein could provide theoretical basis for eggplant cultivar identification and the protection of intellectual property.

Key words: eggplant, core varieties, SNP marker, fingerprinting

中图分类号:

S641.1

魏庆镇, 王五宏, 胡天华, 胡海娇, 汪精磊, 包崇来. 浙茄类型茄子品种DNA指纹图谱构建[J]. 浙江农业学报, 2019, 31(11): 1863-1870.

WEI Qingzhen, WANG Wuhong, HU Tianhua, HU Haijiao, WANG Jinglei, BAO Chonglai. Construction of DNA fingerprinting of Zhejiang eggplant varieties[J]. , 2019, 31(11): 1863-1870.

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浙茄类型茄子品种DNA指纹图谱构建

Construction of DNA fingerprinting of Zhejiang eggplant varieties

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