枸杞根腐病菌拮抗菌株的筛选与鉴定

doi:10.3969/j.issn.1004-1524.2020.05.14

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (5): 858-865.DOI: 10.3969/j.issn.1004-1524.2020.05.14

枸杞根腐病菌拮抗菌株的筛选与鉴定

张小彦, 何静^*, 侯彩霞, 张树衡

甘肃农业大学林学院,甘肃兰州 730070

收稿日期:2019-11-08 出版日期:2020-05-25 发布日期:2020-05-29
通讯作者: *,何静,E-mail:hejing268@aliyun.com
作者简介:张小彦(1994—),女,甘肃临洮人,硕士研究生,研究方向为森林保护。E-mail:1039507106@qq.com
基金资助:
甘肃省自然科学基金(17JR5RA146); 甘肃农业大学青年导师扶持基金(GAU-QDFC-2018-07); 甘肃农业大学学科建设专项基金(GAU-XKJS-2018-096)

Screening and identification of antagonistic strains of wolfberry root rot

ZHANG Xiaoyan, HE Jing^*, HOU Caixia, ZHANG Shuheng

College of Forestry, Gansu Agricultural University, Lanzhou 730070, China

Received:2019-11-08 Online:2020-05-25 Published:2020-05-29

摘要/Abstract

摘要： 枸杞病虫害是影响枸杞产业发展的重要因素,其中枸杞根腐病是发生最普遍的病害之一。本研究从发病严重的枸杞种植园中,采集健康枸杞植株根际土样,通过常规稀释分离法进行菌株分离;以枸杞根腐病主要致病菌——腐皮镰孢菌(Fusarium solani)为供试菌,采用平板对峙法筛选腐皮镰孢菌的拮抗菌株,并通过形态学和分子生物学方法对拮抗菌株进行了种类鉴定。结果表明:在健康枸杞植株根际土壤样品中分离获得的101株细菌、52株放线菌、30株霉菌和27株酵母菌中,筛选得到对枸杞根腐病菌具有抑制效果的菌株HYM-19和HYC-7,其中菌株HYM-19抑菌率为52.29%,菌株HYC-7的抑菌率为39.80%。经鉴定HYM-19为中华根瘤菌(Sinorhizobium sp.),HYC-7为绿僵菌(Metarhizium sp.)。该研究结果可为有效利用土壤微生物防治枸杞根腐病提供理论依据。

关键词: 枸杞, 根腐病, 拮抗菌株, 筛选, 鉴定

Abstract: Wolfberry diseases and insect pests are important factors affecting the development of wolfberry industry, among which the root rot of wolfberry is a common disease. In this study, healthy wolfberry rhizosphere soil samples were selected from seriously diseased wolfberry plantations and strains were isolated by conventional dilution method. Fusarium solani, the main pathogen of wolfberry root rot, was selected as the tested strain by plate confrontation method, and the antagonistic strain was identified by morphology and molecular biology. The results showed that 101 strains of bacteria, 52 strains of actinomycetes, 30 strains of mycetes and 27 strains of saccharomycetes were isolated from rhizosphere soil samples of healthy wolfberry. Two antagonistic strains, HYM-19 and HYC-7, were screened and identified as Sinorhizobium sp. and Metarhizium sp., respectively. The results provided a theoretical basis for the effective use of soil microorganisms to manage root rot of wolfberry.

Key words: wolfberry, root rot, antagonistic bacteria, screening, identification

中图分类号:

S436.639

张小彦, 何静, 侯彩霞, 张树衡. 枸杞根腐病菌拮抗菌株的筛选与鉴定[J]. 浙江农业学报, 2020, 32(5): 858-865.

ZHANG Xiaoyan, HE Jing, HOU Caixia, ZHANG Shuheng. Screening and identification of antagonistic strains of wolfberry root rot[J]. , 2020, 32(5): 858-865.

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枸杞根腐病菌拮抗菌株的筛选与鉴定

Screening and identification of antagonistic strains of wolfberry root rot

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