生防菌株PF-1基于全基因组数据的分类鉴定及拮抗能力分析

doi:10.3969/j.issn.1004-1524.2020.10.10

浙江农业学报 ›› 2020, Vol. 32 ›› Issue (10): 1816-1822.DOI: 10.3969/j.issn.1004-1524.2020.10.10

生防菌株PF-1基于全基因组数据的分类鉴定及拮抗能力分析

戚家明^1,2,3, 孙杉杉^1,2,3, 张东旭^1,4,*, 肖建中⁴, 徐志文^1,2,3

1.领先生物农业股份有限公司,河北秦皇岛 066004;
2.河北省农业生物技术创新中心,河北秦皇岛 066004;
3.河北省农业农村厅植物营养与海洋功能肥料重点实验室,河北秦皇岛 066004;
4.丽水学院生态学院,浙江丽水 323000

收稿日期:2020-01-03 出版日期:2020-10-25 发布日期:2020-11-16
通讯作者: *张东旭,E-mail:zhang.dongxu@leadst.cn
作者简介:戚家明(1992—),男,河北秦皇岛人,硕士,主要从事微生物发酵和生物防治技术研究。E-mail:qi.jiaming@leadst.cn
基金资助:
河北省产业创新创业团队(169A76114H); 浙江省重点研发计划(2018C02031); 浙江省自然科学基金(LY15C200012); 丽水市高层次人才项目(2016RC01)

Identification and biocontrol activity analysis of biocontrol strain PF-1 based on genome-wide data

QI Jiaming^1,2,3, SUN Shanshan^1,2,3, ZHANG Dongxu^1,4,*, XIAO Jianzhong⁴, XU Zhiwen^1,2,3

1. Leading Bio-Agricultural Co., Ltd., Qinhuangdao 066004, China;
2. Hebei Agricultural Biotechnology Innovation Center, Qinhuangdao 066004, China;
3. Key Laboratory of Plant Nutrition and Marine Functional Fertilizer, Agricultural and Rural Department of Hebei Province, Qinhuangdao 066004, China;
4. College of Ecology, Lishui University, Lishui 323000, China

Received:2020-01-03 Online:2020-10-25 Published:2020-11-16

摘要/Abstract

摘要： 基于全基因组数据,确定生防菌株PF-1的分类地位,验证其对植物病原菌的拮抗作用以及挖掘其潜在的生防功能。通过全基因组中16S rRNA基因序列的系统发育分析及基因组分析方法确定生防菌株PF-1的分类地位,通过平板对峙方法研究其对马铃薯枯萎病菌和棉花黄萎病菌的拮抗能力;利用antiSMASH软件分析和预测菌株PF-1的抗生素相关基因并挖掘其生防潜力。基于16S rRNA基因序列的系统发育分析及基因组分析结果,PF-1被鉴定为防御假单胞菌(Pseudomonas protegens),同时发现PF-1基因组中存在15种次生代谢产物基因簇,具有良好的抑制病原菌生长和提高植物抗病性的能力,在农业中具有良好的应用前景。

关键词: 防御假单胞菌, 拮抗能力, 基因组, 基因簇

Abstract: Based on the genome-wide data, the taxonomic status of biocontrol strain PF-1, which was isolated from soil, was clarified. The antagonistic effect of biocontrol strain PF-1 on multiple plant pathogens was validated to uncover its potential biocontrol functions. Phylogenetic trees were constructed by 16S rRNA gene sequences analysis to identify biocontrol strain PF-1. Furthermore, whole-genome sequencing analysis was also employed to determine the taxonomic status of strain PF-1. Plate isolate-antagonistic experiments were applied to explore the antagonistic effect of strain PF-1 on Verticillium dahliae Kleb and Fusarium oxysporum. The antibiotic genes of strain PF-1 were figured out among secondary metabolite biosynthesis gene clusters predicted by antiSMASH. According to the results of phylogenetic trees, average nucleotide identity and digital DNA-DNA hybridization, PF-1 was characterized as Pseudomonas protegens, with 15 secondary metabolite gene clusters in genome, exhibited a good ability to inhibit the growth of plant pathogens. Pseudomonas protegens PF-1 has strong antagonistic ability against plant pathogens and good prospects for agricultural applications.

Key words: Pseudomonas protegens, antagonistic ability, genome, gene cluster

中图分类号:

S476

戚家明, 孙杉杉, 张东旭, 肖建中, 徐志文. 生防菌株PF-1基于全基因组数据的分类鉴定及拮抗能力分析[J]. 浙江农业学报, 2020, 32(10): 1816-1822.

QI Jiaming, SUN Shanshan, ZHANG Dongxu, XIAO Jianzhong, XU Zhiwen. Identification and biocontrol activity analysis of biocontrol strain PF-1 based on genome-wide data[J]. , 2020, 32(10): 1816-1822.

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生防菌株PF-1基于全基因组数据的分类鉴定及拮抗能力分析

Identification and biocontrol activity analysis of biocontrol strain PF-1 based on genome-wide data

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