缢蛏早期耐高温家系建立及抗氧化能力测定

doi:10.3969/j.issn.1004-1524.2022.02.07

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (2): 266-274.DOI: 10.3969/j.issn.1004-1524.2022.02.07

缢蛏早期耐高温家系建立及抗氧化能力测定

申奔龙(), 薛宝宝, 孟德龙, 沈和定^*()

上海海洋大学水产种质资源发掘与利用教育部重点实验室,水产科学国家级实验教学示范中心,海洋动物系统分类与进化上海高校重点实验室, 上海 201306

收稿日期:2020-09-04 出版日期:2022-02-25 发布日期:2022-03-02
通讯作者: 沈和定
作者简介:沈和定,E-mail: hdshen@shou.edu.cn
申奔龙(1995—),男,山东任城人,硕士研究生,从事海洋贝类育种研究。E-mail: shenbenlong@126.com
基金资助:
上海高校知识服务平台上海海洋大学水产动物遗传育种中心(ZF1206);国家重点研发计划(2020YFD0900800)

Establishment of a high temperature resistant family and determination of antioxidant capacity of razor clam Sinonovacula constricta

SHEN Benlong(), XUE Baobao, MENG Delong, SHEN Heding^*()

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, National Demonstration Center for Experimental Fisheries Science Education,Key Laboratory of Marine Animal Taxonomy and Evolution, Shanghai Ocean University, Shanghai 201306, China

Received:2020-09-04 Online:2022-02-25 Published:2022-03-02
Contact: SHEN Heding

摘要/Abstract

摘要：

为筛选缢蛏(Sinonovacula constricta)耐高温新品系,该实验以缢蛏新品种申浙1号为基础群,先期建立了16个全同胞家系,并在第2、8、22、36日对各家系的生长进行了监测,在稚贝期分别挑选了3组壳长较长和3组壳长较短家系进行了半致死温度试验与抗氧化能力测定。结果表明:家系6、14在稚贝期的成活率显著高于其他家系(P<0.05),其中家系14的成活率最高;在半致死温度试验中,家系16的半致死温度最高达到36.64 ℃,且壳长较长家系与壳长较短家系半致死温度差异显著(P<0.05);各家系过氧化物酶(POD)、总抗氧化能力(T-AOC)、谷胱甘肽(GSH)随温度升高而升高,壳长较长家系的3种抗氧化能力在高温下均显著高于壳长较短家系(P<0.05)。通过家系选育和高温实验挑选具有抗逆性的耐高温后代,并通过抗氧化能力的测定在生理生化角度证明了家系挑选的必要性。在综合评估了各家系的壳长、存活率、耐热性和抗氧化能力等数据后最终确定家系14是适合生产的最优家系,家系15、16可用作备选家系。

关键词: 缢蛏, 家系, 高温

Abstract:

In order to continue to select the new high-temperature tolerant varieties s of razor clam Sinonovacula constricta Shenzhe No. 1, 16 full-sib families were established in the early stage of this experiment, and the growth of each family was recorded on the 2, 8, 12, 24, and 36 days. In the spat stage, 3 families of longer shell length and 3 families of shorter shell length were selected, and a total of 6 families were selected to the semi-lethal temperature experiment and determination of antioxidant capacity. The results showed that the survival rate of family 6 and 14 was significantly higher than that of other families at the spat stage, and the survival rate of family 14 was the highest. In the semi-lethal temperature test, the semi-lethal temperature of family 16 reached the highest of 36.64 ℃, and there was a significant difference in the semi-lethal temperature between families with long shell length and families with short shell length (P<0.05). Peroxidase (POD), total antioxidant capacity (T-AOC) and glutathione (GSH) increased with the increase of temperature. The three antioxidant indices of families with longer shell length were significantly higher than those of families with shorter shell length at high temperature (P<0.05). Through family selection and high temperature experiments, the offspring with resistance to high temperature were selected, and the determination of antioxidant capacity proved the necessity of family selection from the perspective of physiology and biochemistry. After comprehensively evaluating the shell length, survival rate and heat tolerance data of each family, it is finally determined that family 14 was the best family suitable for production, and family 15, family 16 could be used as candidate families.

Key words: Sinonovacula constricta, high temperature tolerance, family

中图分类号:

S968.31

申奔龙, 薛宝宝, 孟德龙, 沈和定. 缢蛏早期耐高温家系建立及抗氧化能力测定[J]. 浙江农业学报, 2022, 34(2): 266-274.

SHEN Benlong, XUE Baobao, MENG Delong, SHEN Heding. Establishment of a high temperature resistant family and determination of antioxidant capacity of razor clam Sinonovacula constricta[J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 266-274.

图/表 6

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家系编号 Family number	第2天 The 2nd day		第8天 The 8th day		第22天 The 22nd day		第36天 The 36th day
家系编号 Family number	SL/μm	SH/μm	SL/μm	SH/μm	SL/mm	SH/mm	SL/mm	SH/mm
1	135.58 ±8.58 a	105.51 ±5.93 ab	249.40 ±15.53 a	207.73 ±19.01 a	—	—	—	—
2	136.83 ±7.03 a	107.00 ±4.25 ab	252.36 ±20.15 a	209.20 ±19.53 ab	—	—	—	—
3	138.75 ±7.49 ab	109.00 ±5.38 b	261.36 ±12.3 bc	221.20 ±13.37 cde	2.15 ±0.41 ab	1.05 ±0.17 a	4.88 ±0.67 a	1.94 ±0.26 b
4	136.58 ±8.37 a	106.83 ±4.74 ab	261.43 ±9.75 bc	218.36 ±9.68 bcd	2.17 ±0.35 ab	1.06 ±0.16 a	4.97 ±0.54 a	1.99 ±0.22 ab
5	136.75 ±8.49 a	107.75 ±5.17 ab	256.36 ±12.93 ab	212.86 ±12.77 abc	2.07 ±0.22 a	1.03 ±0.11 a	4.76 ±1.16 a	1.90 ±0.46 a
6	138.00 ±8.32 a	107.08 ±4.56 ab	270.53 ±12.83 def	225.36 ±15.03 def	2.52 ±0.44 defg	1.23 ±0.20 bc	6.43 ±0.94 def	2.64 ±0.42 gi
7	138.33 ±7.61 ab	108.08 ±4.94 ab	264.20 ±15.60 bcde	219.86 ±15.78 cd	2.36 ±0.33 bcd	1.18 ±0.21 b	5.68 ±0.65 bc	2.28 ±0.26 cd
8	143.08 ±9.82 b	108.83 ±4.90 ab	271.36 ±12.75 def	225.90 ±12.08 def	2.57 ±0.62defg	1.28 ±0.28 bcd	6.54 ±1.13 defg	2.62 ±0.45 fgi
9	137.83 ±7.49a	107.58 ±4.85 ab	262.53 ±21.66 bcd	218.83 ±21.86 bcd	—	—	—	—
10	137.33 ±7.79 a	108.00 ±4.97 ab	270.53 ±15.49 ef	227.06 ±14.96 def	2.48 ±0.43 cdef	1.27 ±0.19 bcd	6.26 ±0.92 cde	2.51 ±0.36 efg
11	138.08 ±7.84 a	105.66 ±4.56 a	263.70 ±13.05 bcd	220.60 ±12.47 cd	2.24 ±0.26 abc	1.05 ±0.11 a	5.43 ±0.88 b	2.18 ±0.35 bc
12	136.33 ±7.92 a	106.50 ±4.50 ab	269.03 ±14.68 cdef	225.40 ±13.78 def	2.46 ±0.36 cdef	1.23 ±0.18 bc	5.96 ±0.93 bc	2.39 ±0.37 de
13	138.33 ±8.71 ab	106.50 ±4.72 ab	266.36 ±14.43 cdef	216.43 ±38.93 abcd	2.43 ±0.4 cdef	1.26 ±0.25 bcd	6.01 ±1.29 bcd	2.42 ±0.51 def
14	138.91 ±7.43 ab	108.33 ±5.37 ab	275.03 ±14.49 f	232.10 ±12.76 f	2.75 ±0.4 g	1.34 ±0.19 cd	7.04 ±0.72 g	2.83 ±0.29 i
15	139.33 ±7.90 ab	107.91 ±4.96 ab	274.70 ±14.35 f	232.06 ±13.38 f	2.71 ±0.61 fg	1.34 ±0.24 cd	6.93 ±1.31 fg	2.78 ±0.52 i
16	139.83 ±10.01 ab	107.16 ±4.68 ab	273.03 ±13.83 ef	231.03 ±13.31 ef	2.66 ±0.55 efg	1.36 ±0.25 d	6.71 ±0.85 efg	2.69 ±0.33 gi

家系编号 Family number	第2天 The 2nd day		第8天 The 8th day		第22天 The 22nd day		第36天 The 36th day
家系编号 Family number	SL/μm	SH/μm	SL/μm	SH/μm	SL/mm	SH/mm	SL/mm	SH/mm
1	135.58 ±8.58 a	105.51 ±5.93 ab	249.40 ±15.53 a	207.73 ±19.01 a	—	—	—	—
2	136.83 ±7.03 a	107.00 ±4.25 ab	252.36 ±20.15 a	209.20 ±19.53 ab	—	—	—	—
3	138.75 ±7.49 ab	109.00 ±5.38 b	261.36 ±12.3 bc	221.20 ±13.37 cde	2.15 ±0.41 ab	1.05 ±0.17 a	4.88 ±0.67 a	1.94 ±0.26 b
4	136.58 ±8.37 a	106.83 ±4.74 ab	261.43 ±9.75 bc	218.36 ±9.68 bcd	2.17 ±0.35 ab	1.06 ±0.16 a	4.97 ±0.54 a	1.99 ±0.22 ab
5	136.75 ±8.49 a	107.75 ±5.17 ab	256.36 ±12.93 ab	212.86 ±12.77 abc	2.07 ±0.22 a	1.03 ±0.11 a	4.76 ±1.16 a	1.90 ±0.46 a
6	138.00 ±8.32 a	107.08 ±4.56 ab	270.53 ±12.83 def	225.36 ±15.03 def	2.52 ±0.44 defg	1.23 ±0.20 bc	6.43 ±0.94 def	2.64 ±0.42 gi
7	138.33 ±7.61 ab	108.08 ±4.94 ab	264.20 ±15.60 bcde	219.86 ±15.78 cd	2.36 ±0.33 bcd	1.18 ±0.21 b	5.68 ±0.65 bc	2.28 ±0.26 cd
8	143.08 ±9.82 b	108.83 ±4.90 ab	271.36 ±12.75 def	225.90 ±12.08 def	2.57 ±0.62defg	1.28 ±0.28 bcd	6.54 ±1.13 defg	2.62 ±0.45 fgi
9	137.83 ±7.49a	107.58 ±4.85 ab	262.53 ±21.66 bcd	218.83 ±21.86 bcd	—	—	—	—
10	137.33 ±7.79 a	108.00 ±4.97 ab	270.53 ±15.49 ef	227.06 ±14.96 def	2.48 ±0.43 cdef	1.27 ±0.19 bcd	6.26 ±0.92 cde	2.51 ±0.36 efg
11	138.08 ±7.84 a	105.66 ±4.56 a	263.70 ±13.05 bcd	220.60 ±12.47 cd	2.24 ±0.26 abc	1.05 ±0.11 a	5.43 ±0.88 b	2.18 ±0.35 bc
12	136.33 ±7.92 a	106.50 ±4.50 ab	269.03 ±14.68 cdef	225.40 ±13.78 def	2.46 ±0.36 cdef	1.23 ±0.18 bc	5.96 ±0.93 bc	2.39 ±0.37 de
13	138.33 ±8.71 ab	106.50 ±4.72 ab	266.36 ±14.43 cdef	216.43 ±38.93 abcd	2.43 ±0.4 cdef	1.26 ±0.25 bcd	6.01 ±1.29 bcd	2.42 ±0.51 def
14	138.91 ±7.43 ab	108.33 ±5.37 ab	275.03 ±14.49 f	232.10 ±12.76 f	2.75 ±0.4 g	1.34 ±0.19 cd	7.04 ±0.72 g	2.83 ±0.29 i
15	139.33 ±7.90 ab	107.91 ±4.96 ab	274.70 ±14.35 f	232.06 ±13.38 f	2.71 ±0.61 fg	1.34 ±0.24 cd	6.93 ±1.31 fg	2.78 ±0.52 i
16	139.83 ±10.01 ab	107.16 ±4.68 ab	273.03 ±13.83 ef	231.03 ±13.31 ef	2.66 ±0.55 efg	1.36 ±0.25 d	6.71 ±0.85 efg	2.69 ±0.33 gi

缢蛏早期耐高温家系建立及抗氧化能力测定

Establishment of a high temperature resistant family and determination of antioxidant capacity of razor clam Sinonovacula constricta

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