肠道条件和粒径对加工山核桃体外消化的影响

doi:10.3969/j.issn.1004-1524.2022.08.17

浙江农业学报 ›› 2022, Vol. 34 ›› Issue (8): 1734-1742.DOI: 10.3969/j.issn.1004-1524.2022.08.17

肠道条件和粒径对加工山核桃体外消化的影响

邵宇辰¹^,²(), 穆宏磊², 陈杭君², 殷军艺¹, 房祥军², 吴伟杰², 刘瑞玲², 韩延超², 郜海燕¹^,²^,^*()

1.南昌大学食品学院,江西南昌 330036
2.浙江省农业科学院食品科学研究所,农业农村部果品产后处理重点实验室,中国轻工业果蔬保鲜与加工重点实验室,浙江省果蔬保鲜与加工技术研究重点实验室,浙江杭州 310021

收稿日期:2021-03-16 出版日期:2022-08-25 发布日期:2022-08-26
通讯作者: 郜海燕
作者简介:*郜海燕,E-mail: spsghy@163.com
邵宇辰(1996—),女,安徽宿州人,硕士研究生,研究方向为营养与食品卫生学。E-mail: F1232123@126.com
基金资助:
浙江省重点研发计划(2018C02006);国家自然科学基金(31571907)

Effects of intestinal conditions and particle size on in vitro digestion of processed Carya cathayensis Sarg.

SHAO Yuchen¹^,²(), MU Honglei², CHEN Hangjun², YIN Junyi¹, FANG Xiangjun², WU Weijie², LIU Ruiling², HAN Yanchao², GAO Haiyan¹^,²^,^*()

1. School of Food Science, Nanchang University, Nanchang 330036, China
2. Key Laboratory of Postharvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-03-16 Online:2022-08-25 Published:2022-08-26
Contact: GAO Haiyan

摘要/Abstract

摘要：

根据健康成人和胰腺外分泌功能不全(exocrine pancreatic insufficiency, EPI)人群的肠道条件,设置不同的胆盐浓度、肠道pH和胰脂酶浓度,分析加工山核桃基质降解率(matrix degradation rate,MDI)、脂肪和蛋白质消化率、总酚和抗氧化活性,探究不同肠道条件和粒径大小对加工山核桃体外消化的影响。结果显示,粒径大小对加工山核桃体外消化影响最显著,小颗粒加工山核桃MDI、抗氧化活性以及脂肪和蛋白质消化率均高于大颗粒加工山核桃。pH和胆盐浓度与脂肪消化和抗氧化活性相关,pH 7,胆盐浓度为10 mmol·L^-1时,脂肪消化效果更好;胰脂酶浓度越高,脂肪和蛋白质消化率越高。因此,EPI人群可适量摄入补充益生元、胰酶制剂,提高对山核桃制品营养素消化利用率。

关键词: 山核桃, 肠道条件, 粒径大小, 体外消化, 抗氧化活性

Abstract:

This article explored the effects of different intestinal conditions and particle size on in vitro digestion of processed Carya cathayensis Sarg.(CCS), matrix degradation rate (MDI), digestibility of fat and protein, total phenols and antioxidant activity of processed CCS were analyzed under different bile salt concentration, intestinal pH values and pancreatic lipase concentrations according to the intestinal conditions of healthy adults and people with exocrine pancreatic insufficiency (EPI). The results showed particle size had the most significant effect on processed CCS in vitro digestion, and the MDI, antioxidant activity and digestibility of fat and protein of small particle processed CCS were higher than those of large particle processed CCS. The pH and bile salt were associated with fat digestion and antioxidant activity. When pH value was 7 and bile salt concentration was 10 mmol·L^-1, the fat digestion effect was better. The digestibility of fat and protein would be high when the concentration of pancreatic lipase was high. Therefore, people with EPI can take in the appropriate amount of prebiotics and trypsin preparation to improve the nutrient digestion and utilization rate of CCS products.

Key words: Carya cathayensis Sarg., intestinal condition, particle size, in vitro digestion, antioxidant activity

中图分类号:

TS255.6

邵宇辰, 穆宏磊, 陈杭君, 殷军艺, 房祥军, 吴伟杰, 刘瑞玲, 韩延超, 郜海燕. 肠道条件和粒径对加工山核桃体外消化的影响[J]. 浙江农业学报, 2022, 34(8): 1734-1742.

SHAO Yuchen, MU Honglei, CHEN Hangjun, YIN Junyi, FANG Xiangjun, WU Weijie, LIU Ruiling, HAN Yanchao, GAO Haiyan. Effects of intestinal conditions and particle size on in vitro digestion of processed Carya cathayensis Sarg.[J]. Acta Agriculturae Zhejiangensis, 2022, 34(8): 1734-1742.

图/表 7

表1 影响因素及交互作用对MDI、脂肪消化率、蛋白质消化率、总酚、DPPH清除率和FRAP的影响

Table 1 Influence of various factors and interactions on MDI,fat digestibility, protein digestibility, total phenols digestibility, DPPH radical and FARP antioxidant

影响因素与相互作用 Influence factors and interaction	粒径 Particle size	胆盐浓度 Bile salt concentration	pH	粒径-胆盐浓度 Particle size-Bile salt concentration	粒径-pH Particle size-pH	胆盐浓度-pH Bile salt concentration-pH	粒径-胆盐浓度-pH Particle size-Bile salt concentration-pH
MDI/%	471.44^***	6.44^*	5.97^*	1.07	0.01	6.44^*	0.32
脂肪消化率	4 602.43^***	26.83^***	454.04^***	4.04	9.05^**	69.56^***	11.55^**
Fat digestibility/%
蛋白质消化率	29.79^***	17.22^***	2.66	7.12^*	4.83^*	0.27	1.99
Protein digestibility/%
总酚	10.53^**	2.04	5.37^*	1.36	0.60	3.20	1.17
Total phenols/(mg·g^-1)
DPPH清除率	1 723.63^***	34.80^***	188.28^***	23.65^***	10.98^**	0.45	4.45
Scavenging of DPPH radical/%
FRAP/ (μmol·g^-1)	304.21^***	33.03^***	76.17^***	88.17^***	11.95^**	3.33	12.99^**

图1 粒径和肠道条件对山核桃体外消化MDI的影响不同处理间没有相同小写字母表示差异显著(P<0.05)。下同。

Fig.1 Effects of particle size and intestinal conditions on in vitro MDI of Carya cathayensis Sarg. The bars without the same lowercase letters show the significant difference (P<0.05). The same as below.

图2 粒径和肠道条件对山核桃体外消化脂肪消化率的影响

Fig.2 Effects of particle size and intestinal conditions on in vitro fat digestibility of Carya cathayensis Sarg.

图3 粒径和肠道条件对山核桃体外消化蛋白质消化率的影响

Fig.3 Effects of particle size and intestinal conditions on in vitro protein digestibility of Carya cathayensis Sarg.

图4 粒径和肠道条件对山核桃体外消化总酚的影响

Fig.4 Effects of particle size and intestinal conditions on in vitro total phenols of Carya cathayensis Sarg.

图5 粒径和肠道条件对山核桃体外消化DPPH清除率的影响

Fig.5 Effects of particle size and intestinal conditions on in vitro scavenging of DPPH radical of Carya cathayensis Sarg.

图6 粒径和肠道条件对山核桃体外消化FRAP能力的影响

Fig.6 Effects of particle size and intestinal conditions on in vitro FRAP antioxidant ability of Carya cathayensis Sarg.

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肠道条件和粒径对加工山核桃体外消化的影响

Effects of intestinal conditions and particle size on in vitro digestion of processed Carya cathayensis Sarg.

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